Complete genome sequencing and comparative genome characterization of the lignocellulosic biomass degrading bacterium Pseudomonas stutzeri MP4687 from cattle rumen

Patel, Maulik; Patel, Hiral; Vohra, Nasim; Dave, Sanjay

doi:10.1016/j.btre.2020.e00530

Cited by 8 publications

(3 citation statements)

References 40 publications

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“…The biomass of P. kudriavzevii is rich in glycerol [42], which might be used as a substrate to promote the growth of Anaerovibrio. Our data showed that isolate 8 feeding increased the relative abundance of Pseudomonas, which can perform functions including hydrocarbon degradation, xenobiotic degradation, nitrification, denitrification, cellulose degradation and lipolysis [43][44][45]. The higher proportion of Pseudomonas may improve feed digestibility in Hu sheep.…”

Section: Discussionmentioning

confidence: 72%

Isolation and Characterization of Ruminal Yeast Strain with Probiotic Potential and Its Effects on Growth Performance, Nutrients Digestibility, Rumen Fermentation and Microbiota of Hu Sheep

Wang

Jin

et al. 2022

JoF

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Yeast strains are widely used in ruminant production. However, knowledge about the effects of rumen native yeasts on ruminants is limited. Therefore, this study aimed to obtain a rumen native yeast isolate and investigate its effects on growth performance, nutrient digestibility, rumen fermentation and microbiota in Hu sheep. Yeasts were isolated by picking up colonies from agar plates, and identified by sequencing the ITS sequences. One isolate belonging to Pichia kudriavzevii had the highest optical density among these isolates obtained. This isolate was prepared to perform an animal feeding trial. A randomized block design was used for the animal trial. Sixteen Hu sheep were randomly assigned to the control (CON, fed basal diet, n = 8) and treatment group (LPK, fed basal diet plus P. kudriavzevii, CFU = 8 × 109 head/d, n = 8). Sheep were housed individually and treated for 4 weeks. Compared to CON, LPK increased final body weight, nutrient digestibility and rumen acetate concentration and acetate-to-propionate ratio in sheep. The results of Illumina MiSeq PE 300 sequencing showed that LPK increased the relative abundance of lipolytic bacteria (Anaerovibrio spp. and Pseudomonas spp.) and probiotic bacteria (Faecalibacterium spp. and Bifidobacterium spp.). For rumen eukaryotes, LPK increased the genera associated with fiber degradation, including protozoan Polyplastron and fungus Pichia. Our results discovered that rumen native yeast isolate P. kudriavzevii might promote the digestion of fibers and lipids by modulating specific microbial populations with enhancing acetate-type fermentation.

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Section: Discussionmentioning

confidence: 72%

Isolation and Characterization of Ruminal Yeast Strain with Probiotic Potential and Its Effects on Growth Performance, Nutrients Digestibility, Rumen Fermentation and Microbiota of Hu Sheep

Wang

Jin

et al. 2022

JoF

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“…and Serratia spp. [59][60][61]. They usually produce enzyme systems for lignocellulose degradation, which comprises cellulase, xylanase and cellobiase.…”

Section: Typical Microorganisms For Lignocellulose Degradationmentioning

confidence: 99%

Challenges and Future Perspectives of Promising Biotechnologies for Lignocellulosic Biorefinery

et al. 2021

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Lignocellulose is a kind of renewable bioresource containing abundant polysaccharides, which can be used for biochemicals and biofuels production. However, the complex structure hinders the final efficiency of lignocellulosic biorefinery. This review comprehensively summarizes the hydrolases and typical microorganisms for lignocellulosic degradation. Moreover, the commonly used bioprocesses for lignocellulosic biorefinery are also discussed, including separated hydrolysis and fermentation, simultaneous saccharification and fermentation and consolidated bioprocessing. Among these methods, construction of microbial co-culturing systems via consolidated bioprocessing is regarded as a potential strategy to efficiently produce biochemicals and biofuels, providing theoretical direction for constructing efficient and stable biorefinery process system in the future.

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“…While salicin utilization has been observed in some Pseudomonas strains [ 13 , 14 ], those particular strains have not been identified in association with Populus [ 6 ]. Therefore, we hypothesized that another organism within the microbiome might act as a facilitator of the interaction between Populus and Pseudomonas species.…”

Section: Introductionmentioning

confidence: 99%

Mechanism for Utilization of the Populus-Derived Metabolite Salicin by a Pseudomonas—Rahnella Co-Culture

et al. 2023

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Pseudomonas fluorescens GM16 associates with Populus, a model plant in biofuel production. Populus releases abundant phenolic glycosides such as salicin, but P. fluorescens GM16 cannot utilize salicin, whereas Pseudomonas strains are known to utilize compounds similar to the aglycone moiety of salicin–salicyl alcohol. We propose that the association of Pseudomonas to Populus is mediated by another organism (such as Rahnella aquatilis OV744) that degrades the glucosyl group of salicin. In this study, we demonstrate that in the Rahnella–Pseudomonas salicin co-culture model, Rahnella grows by degrading salicin to glucose 6-phosphate and salicyl alcohol which is secreted out and is subsequently utilized by P. fluorescens GM16 for its growth. Using various quantitative approaches, we elucidate the individual pathways for salicin and salicyl alcohol metabolism present in Rahnella and Pseudomonas, respectively. Furthermore, we were able to establish that the salicyl alcohol cross-feeding interaction between the two strains on salicin medium is carried out through the combination of their respective individual pathways. The research presents one of the potential advantages of salicyl alcohol release by strains such as Rahnella, and how phenolic glycosides could be involved in attracting multiple types of bacteria into the Populus microbiome.

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Complete genome sequencing and comparative genome characterization of the lignocellulosic biomass degrading bacterium Pseudomonas stutzeri MP4687 from cattle rumen

Abstract: Highlights The isolation of P. stutzeri MP4687 from cattle rumen. The complete genome sequencing and comparative analysis of P. stuteri. Adaptation to the rumen environment. The strain has complete LCB hydrolyzing potential.

Cited by 8 publications

References 40 publications

Isolation and Characterization of Ruminal Yeast Strain with Probiotic Potential and Its Effects on Growth Performance, Nutrients Digestibility, Rumen Fermentation and Microbiota of Hu Sheep

Isolation and Characterization of Ruminal Yeast Strain with Probiotic Potential and Its Effects on Growth Performance, Nutrients Digestibility, Rumen Fermentation and Microbiota of Hu Sheep

Challenges and Future Perspectives of Promising Biotechnologies for Lignocellulosic Biorefinery

Mechanism for Utilization of the Populus-Derived Metabolite Salicin by a Pseudomonas—Rahnella Co-Culture

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