Complete Genome Sequences of Two Severe Fever with Thrombocytopenia Syndrome Virus Strains Isolated from a Human and a Dog in the Republic Of Korea

Lee, Sook-Young; Kang, Joon Soon; Jeong, Hyesung; Kim, Won-Meong; Son, Kidong; Kim, Ji Soo; Oh, Sung-Suk; Cho, Yoon‐Kyoung; Jheong, Weon-Hwa; Chae, Joon‐Seok

doi:10.1128/mra.01695-18

Cited by 13 publications

(15 citation statements)

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“…The Korean SFTSV strain KH1 (GenBank accession no. MH491547, MH491548.1, MH491549.1) was kindly provided by Dr. Joon-Seok Chae [ 16 ]. This virus was passaged five times on monolayers of Vero E6 cells in DMEM containing 5% fetal bovine serum (FBS) with antibiotics (penicillin; 100 U/ml, streptomycin; 100 μg/ml).…”

Section: Methodsmentioning

confidence: 99%

Pathogenicity of severe fever with thrombocytopenia syndrome virus in mice regulated in type I interferon signaling

Park

Choi

et al. 2020

Lab Anim Res

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Severe fever with thrombocytopenia syndrome (SFTS) is an emerging zoonotic disease, which causes high fever, thrombocytopenia, and death in humans and animals in East Asian countries. The pathogenicity of SFTS virus (SFTSV) remains unclear. We intraperitoneally infected three groups of mice: wild-type (WT), mice treated with blocking anti-type I interferon (IFN)-α receptor antibody (IFNAR Ab), and IFNAR knockout (IFNAR−/−) mice, with four doses of SFTSV (KH1, 5 × 105 to 5 × 102 FAID50). The WT mice survived all SFTSV infective doses. The IFNAR Ab mice died within 7 days post-infection (dpi) with all doses of SFTSV except that the mice were infected with 5 × 102 FAID50 SFTSV. The IFNAR−/− mice died after infection with all doses of SFTSV within four dpi. No SFTSV infection caused hyperthermia in any mice, whereas all the dead mice showed hypothermia and weight loss. In the WT mice, SFTSV RNA was detected in the eyes, oral swabs, urine, and feces at 5 dpi. Similar patterns were observed in the IFNAR Ab and IFNAR−/− mice after 3 dpi, but not in feces. The IFNAR Ab mice showed viral shedding until 7 dpi. The SFTSV RNA loads were higher in organs of the IFNAR−/− mice compared to the other groups. Histopathologically, coagulation necrosis and mononuclear inflammatory cell infiltration in the liver and white pulp atrophy in the spleen were seen as the main lesions in the IFN signaling lacking mice. Immunohistochemically, SFTSV antigens were mainly detected in the marginal zone of the white pulp of the spleen in all groups of mice, but more viral antigens were observed in the spleen of the IFNAR−/− mice. Collectively, the IFN signaling-deficient mice were highly susceptible to SFTSV and more viral burden could be demonstrated in various excreta and organs of the mice when IFN signaling was inhibited.

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Section: Methodsmentioning

confidence: 99%

Pathogenicity of severe fever with thrombocytopenia syndrome virus in mice regulated in type I interferon signaling

Park

Choi

et al. 2020

Lab Anim Res

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“…The initial case mortality rate of SFTS reached up to 30% in the early years, though the estimated mortality rate has decreased to 12% in China while a high fatality rate was still reported in Japan and Korea ( Robles et al., 2018 ). Humans are the main host for SFTSV, people become infected mainly via tick-bites, then lead to human-to-human transmission, apart from humans, other mammals and rodents also could be SFTSV hosts ( Lee et al., 2019 ; Chung et al., 2020 ), direct contact with the body fluids of infected animals leads to SFTSV infections in humans as well ( Kobayashi et al., 2020 ). A recent study detected SFTSV RNA in semen following its disappearance from serum presence, indicating a possible sexual transmission of SFTSV ( Koga et al., 2019 ), a report pointed out that the formation of aerosols was also a potential transmission route of SFTSV ( Moon et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

The AST/ALT Ratio (De Ritis Ratio) Represents an Unfavorable Prognosis in Patients in Early-Stage SFTS: An Observational Cohort Study

Wang

Zhang

et al. 2022

Front. Cell. Infect. Microbiol.

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BackgroundSevere fever with thrombocytopenia syndrome (SFTS), a widely prevalent infectious disease caused by severe fever with thrombocytopenia syndrome virus (SFTSV) that carries with it a high mortality rate, has emerged to be a public health concern. This study aimed to investigate the epidemiological and clinical characteristics of patients infected with SFTSV, seeking novel prognostic risk factors for SFTS.MethodsIn this retrospective and cross-sectional study, confirmed SFTS patients from the First Affiliated Hospital of Anhui Medical University were enrolled from September 1, 2019, to December 12, 2020. Cases were analyzed for epidemiological, demographic, clinical, and laboratory data. Logistic regression models were used to assess the association between predictors and outcome variables. A generalized additive mixed model (GAMM) was conducted to analyze the trending shift of aspartate aminotransferase/alanine transaminase-ratio (AST/ALT-ratio) and platelet (PLT) in SFTS patients treated with ribavirin. p values ≤ 0.05 were considered statistically significant.ResultsClinical and laboratory results of 107 hospitalized patients with SFTSV infection were retrospectively described. The mean age at onset of disease was 60.38 ± 11.29 years old and the ratio between male and female was 1:1.2. Fever and thrombocytopenia are hallmark features of SFTS. Furthermore, multiple cases also experienced neurological complications, gastrointestinal/skeletal muscle symptoms together with other non-specific clinical manifestations; laboratory dataset outcomes reported dysregulated levels for routine blood biomarkers, coagulation function, and biochemistry. Overall, 107 patients were segregated into two groups according to patient condition at the clinical endpoint (survivors/non-survivors). SFTS survivors had a higher level of PLT- counts, total protein (TP), and estimated glomerular filtration rate (eGFR), while levels of activated partial thromboplastin time (APTT), thrombin time (TT), D-dimer (D-D), fibrinogen degradation products (FDP), ALT, AST, AST/ALT-ratio, creatinine (Cr), creatine phosphokinase (CK) and procalcitonin (PCT) was higher in non-survivors. Results from univariate Cox regression revealed that elevated levels of FDP, TT, AST/ALT-ratio, PCT, as well as decreased eGFR level and presence of central nervous system symptoms (CNS), were significant predictors for SFTS prognostic, results from multivariate logistic regression analysis in three adjusted models showed AST/ALT-ratio and PCT were independent risk factors for the prognosis of SFTS patients. Kaplan–Meier survival analysis showed that SFTS patients with AST/ALT-ratio >2.683 were associated with a shorter futime (means survival time), therefore indicating an unfavorable prognosis. Treatment with ribavirin could increase PLT count while decreasing AST/ALT-ratio within SFTS patients.ConclusionSFTS is an emerging infectious disease, possibly leading to multiple-organ injury; AST/ALT-ratio was an independent risk factor for the prognosis of SFTS patients. Further investigation should be performed in order to gain more knowledge on this disease and guide clinical management.

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“…Ticks such as Haemaphysalis longicornis and Rhipicephalus microplus are implicated as the prominent vectors for transmission while human-to-human transmission through contact with blood or body fluids of patients with SFTS was reported (5,6). Furthermore, recently this infection was also reported in pets (7)(8)(9). Since the first SFTS case was reported in China in 2009, the incidence of SFTS has expanded to at least 15 provinces in China (10) and has been reported in South Korea, in Japan, and recently in Vietnam, suggesting that the endemic area is expanding (10).…”

Section: Introductionmentioning

confidence: 99%

A single-domain antibody inhibits SFTSV and mitigates virus-induced pathogenesis in vivo

Wu¹,

Li²,

Huang³

et al. 2020

JCI Insight

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Complete Genome Sequences of Two Severe Fever with Thrombocytopenia Syndrome Virus Strains Isolated from a Human and a Dog in the Republic Of Korea

Cited by 13 publications

References 13 publications

Pathogenicity of severe fever with thrombocytopenia syndrome virus in mice regulated in type I interferon signaling

Pathogenicity of severe fever with thrombocytopenia syndrome virus in mice regulated in type I interferon signaling

The AST/ALT Ratio (De Ritis Ratio) Represents an Unfavorable Prognosis in Patients in Early-Stage SFTS: An Observational Cohort Study

A single-domain antibody inhibits SFTSV and mitigates virus-induced pathogenesis in vivo

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