Complete Genome Sequences of
 Curtobacterium
 ,
 Pantoea
 ,
 Erwinia
 , and Two Pseudomonas sp. Strains, Isolated from Apple Flower Stigmas from Connecticut, USA

Cui, Zhipeng; Steven, Blaire; Zeng, Quan

doi:10.1128/mra.00154-21

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…The genus Curtobacterium is a member of the Mcrobacteriaceae family and the phylum Actinomycetota (formerly known as Actinobacteria) [1]. Certain Curtobacterium strains are plant pathogenic [2], while many are categorized as either rhizobacteria that promote plant development or endophytes that potentially reduce the host's abiotic stresses [3][4][5]. The C. flaccumfaciens species includes a number of closely associated plant pathogens of economic and agricultural significance that are divided into different pathovars with varying host levels.…”

Section: Introductionmentioning

confidence: 99%

Morphological, biochemical, and molecular identification of Curtobacterium flaccumfaciens isolated from Olea europaea trees

Yaseen Taha,

Qasim Hasan

2024

UNEC j. eng. appl. sci.

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Curtobacterium flaccumfaciens induces a bacterial wilt disease in many kinds of plants, including Olea europaea. The current study intends to isolate and identify C. flaccumfaciens, from the nodes of O. europaea based on morphological, biochemical and molecular features. The pure colonies were subjected to multiple tests included gram stain, motility, oxidase, catalase and antibiotic sensitivity. The findings showed that C. flaccumfaciens strains were motile gram-positive short rods, catalase-positive, oxidase-negative and catalase-positive. C. flaccumfaciens sensitivity against 9 antibiotics was tested using the disc diffusion method. The results revealed that the 5 strains varied in their antibiotic resistance pattern, however, SHGH3 strain is more sensitive to the antibiotics tested compared to the other strains and Meropenem is more efficient than others. The 16S rRNA sequences of the strains SHGH1, SHGH2, SHGH3, SHGH4, and SHGH5 strains were submitted to NCBI and given the accession numbers: OQ799009, OQ7990010, OQ799011, OQ799012, OR230004, respectively. Sequences showed a homology of (99.49%, 97.36%, 99.20%, 99.00%, and 99.32%,) with deposited sequences in GenBank carrying the accessions: KP898898, MT323132, KT614051, MK389451, and MN826580, respectively. The findings of the current study helps to better understanding C. flaccumfaciens in morphological, molecular, and biochemical aspects and shows for the first time the isolation of C. flaccumfaciens from the nodes of O. europaea.

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Section: Introductionmentioning

confidence: 99%

Morphological, biochemical, and molecular identification of Curtobacterium flaccumfaciens isolated from Olea europaea trees

Yaseen Taha,

Qasim Hasan

2024

UNEC j. eng. appl. sci.

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Complete Genome Sequence of Curtobacterium sp. C1, a Beneficial Endophyte with the Potential for In-Plant Salinity Stress Alleviation

Hung

Chiu

Huang

et al. 2022

MPMI

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Investigating the Biocontrol Potential of the Natural Microbiota of the Apple Blossom

2022

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Erwinia amylovora, the causative agent of fire blight, leads to important economic losses of apple and pear crops worldwide. This study aimed to investigate the potential of the resident microbiota of the apple blossom in combatting plant disease-causing organisms, with a focus on controlling fire blight. We obtained 538 isolates from sites around Canton Zurich, which we tested for activity against Pectobacterium carotovorum and E. amylovora. We also evaluated the isolates’ activity against oomycete and fungal pathogens. Nine isolates showed activity against P. carotovorum, and eight of these against E. amylovora. Furthermore, 117 showed antifungal, and 161 anti-oomycete, activity. We assigned genera and in some cases species to 238 of the isolates by sequencing their 16S RNA-encoding gene. Five strains showed activity against all pathogens and were tested in a detached apple model for anti-E. amylovora activity. Of these five strains, two were able to antagonize E. amylovora, namely Bacillus velezensis #124 and Pantoea agglomerans #378. We sequenced the P. agglomerans #378 genome and analyzed it for secondary metabolite clusters using antiSMASH, revealing the presence of a putative bacteriocin cluster. We also showed that B. velezensis #124 exhibits strong activity against three different fungi and two oomycetes in vitro, suggesting a broader capacity for biocontrol. Our results showcase the protective potential of the natural apple blossom microbiota. We isolated two candidate biocontrol strains from apple blossoms, suggesting that they might persist at the most common entry point for the causative agent of fire blight. Furthermore, they are probably already part of the human diet, suggesting they might be safe for consumption, and thus are promising candidates for biocontrol applications.

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Complete Genome Sequences of Curtobacterium , Pantoea , Erwinia , and Two Pseudomonas sp. Strains, Isolated from Apple Flower Stigmas from Connecticut, USA

Cited by 3 publications

References 9 publications

Morphological, biochemical, and molecular identification of Curtobacterium flaccumfaciens isolated from Olea europaea trees

Morphological, biochemical, and molecular identification of Curtobacterium flaccumfaciens isolated from Olea europaea trees

Complete Genome Sequence of Curtobacterium sp. C1, a Beneficial Endophyte with the Potential for In-Plant Salinity Stress Alleviation

Investigating the Biocontrol Potential of the Natural Microbiota of the Apple Blossom

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