Complete Genome Sequences of Getah Virus Strains Isolated from Horses in 2016 in Japan

Nemoto, Manabu; Bannai, Hiroshi; Ochi, Akihiro; Niwa, Hidekazu; Murakami, Satoshi; Tsujimura, Koji; Yamanaka, Takashi; Kokado, Hiroshi; Kondo, Takashi

doi:10.1128/genomea.00750-17

Cited by 7 publications

(3 citation statements)

References 11 publications

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“…GETV infections can cause fever, rashes, and edema of the hindlegs in horses ( 15 ), fetal death and reproductive disorders in pigs ( 16 ) as well as fever, anorexia, depression, neurologic symptoms, and death in foxes ( 17 ). GETV infections in horses and pigs have been reported several times in Japan since the 1970's ( 18 – 21 ), and the outbreak of GETV infections in horses were reported in 1990 in India ( 22 ). In China, GETV is widely distributed in 15 provinces ranging from the southwest to northern areas of China since it was first identified from mosquitos in Hainan province, and it has caused several outbreaks in animals in recent years ( 2 , 7 , 17 , 23 – 28 ).…”

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confidence: 99%

Emergence and Phylogenetic Analysis of a Getah Virus Isolated in Southern China

Ren

Wang

et al. 2020

Front. Vet. Sci.

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Getah virus (GETV) has caused many outbreaks in animals in recent years. Monitoring of the virus and its related diseases is crucial to control the transmission of the virus. In the summer of 2018, we conducted routine tests on clinical samples from different pig farms in Guangxi province, South China, and isolated and characterized a GETV strain, named GX201808. Cytopathic effects were observed in BHK-21 cells inoculated with GX201808. The expression of E2 protein of GETV could be detected in virus-infected cells by indirect immunofluorescence assays. Electron microscopic analysis showed that the virus particles were spherical and ~70 nm in diameter with featured surface fibers. The multistep growth curves showed the virus propagated well in the BHK-21 cells. Molecular genetic analysis revealed that GX201808 belongs to Group 3, represented by Kochi-01-2005 isolated in Japan in 2005, and it clustered closely with the recently reported Chinese strains isolated from pigs, cattle, and foxes. A comparison of the identities of nucleotides and amino acids in the coding regions demonstrated that the GX201808 showed the highest amino acid identity (99.6%) with the HuN1 strain, a highly pathogenic isolate resulting in an outbreak of GETV infection in swine herds in Hunan province in 2017. In the present study, GETV was identified and isolated for the first time in Guangxi province of southern China, suggesting that future surveillance of this virus should be strengthened.

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Section: Introductionmentioning

confidence: 99%

Emergence and Phylogenetic Analysis of a Getah Virus Isolated in Southern China

Ren

Wang

et al. 2020

Front. Vet. Sci.

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“…Over the subsequent 30 years, no GETV outbreaks were observed among racehorses in Japan. However, GETV outbreaks occurred suddenly from 2014 to 2016 at the Miho Training Center among racehorses which had been vaccinated against GETV [ 2 , 18 , 20 ].…”

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“…One study reported that GETV was unexpectedly isolated from Culex tritaeniorhyncus mosquitoes at high minimum infection rates in Nagasaki Prefecture in 2012 [ 13 ]. This GETV strain 12IH26 isolated from mosquitoes in Nagasaki Prefecture is phylogenetically related to the GETV strains that caused an epidemic among racehorses in 2014 and 2016 [ 20 ], being also closely related to Chinese and South Korean strains, rather than the Kochi/01/2005 strain reported in 2005, which is considered a Japanese domestic strain [ 25 ]. Another study reported an epidemic of GETV infection among wild boar populations in Japan in 2012 [ 14 ].…”

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Seroconversion of anti-Getah virus antibody among Japanese native Noma horses around 2012

Takeishi

Kuwata

Ono

et al. 2022

The Journal of Veterinary Medical Science

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Getah virus (GETV), an arthropod-borne virus transmitted by mosquitoes, has been isolated from several animals. GETV infection in horses shows clinical signs such as fever, rash, and edema in the leg. Noma horses are one of the eight Japanese native horses. The present study aimed to clarify the occurrence of GETV infection in Noma horses. Serum samples collected from Noma horses were analyzed using a virus neutralization test and enzyme-linked immunosorbent assay and showed that the anti-GETV antibody titers in the samples collected in 2017 were significantly higher than those collected in 2012. We concluded that a seroconversion of anti-GETV antibodies was occurred in the Noma horse population around 2012, providing evidence of the GETV epidemic in Japan circa 2012.

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