Complete genome sequences of Geobacillus sp. Y412MC52, a xylan-degrading strain isolated from obsidian hot spring in Yellowstone National Park

Brumm, Phillip J.; Land, Miriam; Hauser, Loren; Jeffries, Cynthia D.; Chang, Yen‐Hsiang; Mead, David A.

doi:10.1186/s40793-015-0075-0

Cited by 12 publications

(10 citation statements)

References 52 publications

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“…The latter encodes a number of proteins of predicted metabolic relevance, including acyl-CoA dehydrogenase (BCV53_19405), flavin reductase (BCV53_ 19410), and acetaldehye dehydrogenase (BCV53_19435). However, no proline- and hydroxyproline-related metabolic genes were predicted, which is in contrast to pGEOTH01 present in G. thermoglucosidasius C56-YS93 ( 13 ).…”

Section: Genome Announcementmentioning

confidence: 73%

Complete Genome Sequence of Geobacillus thermoglucosidasius NCIMB 11955, the Progenitor of a Bioethanol Production Strain

2016

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Section: Genome Announcementmentioning

confidence: 73%

Complete Genome Sequence of Geobacillus thermoglucosidasius NCIMB 11955, the Progenitor of a Bioethanol Production Strain

2016

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“…Y4.1MC1. This bacteria was collected from a 88 • C water outflow from a hot spring [27]. PlyGspY4 has a predicted N-terminal glucosaminidase CAT domain (PFAM code PF01832) and a C-terminal LysM (PFAM code PF01476) peptidoglycan binding domain ( Figure 2).…”

Section: Discovery and Bioinformatic Analysis Of Thermophile Endolysinsmentioning

confidence: 99%

Thermophile Lytic Enzyme Fusion Proteins that Target Clostridium perfringens

et al. 2019

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Clostridium perfringens is a bacterial pathogen that causes necrotic enteritis in poultry and livestock, and is a source of food poisoning and gas gangrene in humans. As the agriculture industry eliminates the use of antibiotics in animal feed, alternatives to antibiotics will be needed. Bacteriophage endolysins are enzymes used by the virus to burst their bacterial host, releasing bacteriophage particles. This type of enzyme represents a potential replacement for antibiotics controlling C. perfringens. As animal feed is often heat-treated during production of feed pellets, thermostable enzymes would be preferred for use in feed. To create thermostable endolysins that target C. perfringens, thermophile endolysin catalytic domains were fused to cell wall binding domains from different C. perfringens prophage endolysins. Three thermostable catalytic domains were used, two from prophage endolysins from two Geobacillus strains, and a third endolysin from the deep-sea thermophilic bacteriophage Geobacillus virus E2 (GVE2). These domains harbor predicted L-alanine-amidase, glucosaminidase, and L-alanine-amidase activities, respectively and degrade the peptidoglycan of the bacterial cell wall. The cell wall binding domains were from C. perfringens prophage endolysins (Phage LYtic enzymes; Ply): PlyCP18, PlyCP10, PlyCP33, PlyCP41, and PlyCP26F. The resulting fifteen chimeric proteins were more thermostable than the native C. perfringens endolysins, and killed swine and poultry disease-associated strains of C. perfringens.

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“…(Wu et al., ), Geobacillus spp. (Zambare, Bhalla, Muthukumarappan, Sani, & Christopher, ; Brumm, et al., ; Brumm, Land, & Mead, ), Melioribacter spp. (Rakitin, Ermakova, & Ravin, ), and Thermoanaerobacterium spp.…”

Section: Introductionmentioning

confidence: 99%

Microbial diversity of thermophiles with biomass deconstruction potential in a foliage‐rich hot spring

Lee¹,

Goh

Chan

et al. 2018

MicrobiologyOpen

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The ability of thermophilic microorganisms and their enzymes to decompose biomass have attracted attention due to their quick reaction time, thermostability, and decreased risk of contamination. Exploitation of efficient thermostable glycoside hydrolases (GHs) could accelerate the industrialization of biofuels and biochemicals. However, the full spectrum of thermophiles and their enzymes that are important for biomass degradation at high temperatures have not yet been thoroughly studied. We examined a Malaysian Y-shaped Sungai Klah hot spring located within a wooded area. The fallen foliage that formed a thick layer of biomass bed under the heated water of the Y-shaped Sungai Klah hot spring was an ideal environment for the discovery and analysis of microbial biomass decay communities. We sequenced the hypervariable regions of bacterial and archaeal 16S rRNA genes using total community DNA extracted from the hot spring. Data suggested that 25 phyla, 58 classes, 110 orders, 171 families, and 328 genera inhabited this hot spring. Among the detected genera, members of Acidimicrobium, Aeropyrum, Caldilinea, Caldisphaera, Chloracidobacterium, Chloroflexus, Desulfurobacterium, Fervidobacterium, Geobacillus, Meiothermus, Melioribacter, Methanothermococcus, Methanotorris, Roseiflexus, Thermoanaerobacter, Thermoanaerobacterium, Thermoanaerobaculum, and Thermosipho were the main thermophiles containing various GHs that play an important role in cellulose and hemicellulose breakdown. Collectively, the results suggest that the microbial community in this hot spring represents a good source for isolating efficient biomass degrading thermophiles and thermozymes.

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Complete genome sequences of Geobacillus sp. Y412MC52, a xylan-degrading strain isolated from obsidian hot spring in Yellowstone National Park

Cited by 12 publications

References 52 publications

Complete Genome Sequence of Geobacillus thermoglucosidasius NCIMB 11955, the Progenitor of a Bioethanol Production Strain

Complete Genome Sequence of Geobacillus thermoglucosidasius NCIMB 11955, the Progenitor of a Bioethanol Production Strain

Thermophile Lytic Enzyme Fusion Proteins that Target Clostridium perfringens

Microbial diversity of thermophiles with biomass deconstruction potential in a foliage‐rich hot spring

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