Complete Genome Sequences of Four Soil-Derived Isolates for Studying Synthetic Bacterial Community Assembly

Abstract

Here, we report the complete genome sequences of four bacterial soil isolates, Chryseobacterium sp., Stenotrophomonas indicatrix , Pedobacter sp., and Rhodococcus globerulus . These isolates can be used for studying microbial interactions and community assembly in vitro .

Experimental evolution of Bacillus subtilis on Arabidopsis thaliana roots reveals fast adaptation and improved root colonization

“…To this end, we wondered how the pro-longed adaptation of B. subtilis to the plant root environment in the absence of other microbial species affected the ability to colonize the root in the presence of soil microbes. The ancestor and Ev7.3 were tested for their ability to colonize A. thaliana roots in the presence of a synthetic, soil-derived community ( Lozano-Andrade et al., 2021 ). This community comprises four bacterial species, Pedobacter sp., Rhodococcus globerulus , Stenotrophomas indicatrix and Chryseobacterium sp.…”

“…The four bacterial species, Pedobacter sp., Rhodococcus globerulus , Stenotrophomas indicatrix and Chryseobacterium sp., constituting a synthetic, soil-derived community were previously acquired in Dyrehaven, Kongens Lyngby, Denmark (55° 47′ 19.68″ N, 12° 33′ 29.88″ E), as described ( Lozano-Andrade et al., 2021 ). An overview of the strains used in this study is included in the Key Resources Table .…”

Experimental evolution ofBacillus subtilisonArabidopsis thalianaroots reveals fast adaptation and improved root colonization in the presence of soil microbes

“…The four bacterial species, Pedobacter sp., Rhodococcus globerulus, Stenotrophomas indicatrix and Chryseobacterium sp., constituting a synthetic, soil-derived community were previously acquired in Dyrehaven, Kongens Lyngby,Denmark (55° 47' 19.68" N,12° 33' 29.88" E), as described (Lozano-Andrade et al, 2021). An overview of the strains used in this study is included in the Key Resources Table . B. subtilis strains were routinely grown overnight (ON) in Lysogeny Broth (LB; LB-Lennox, Carl Roth, Germany; 10 g/L tryptone, 5 g/L yeast extract and 5 g/L NaCl) at 37 °C while shaking at 220 rpm.…”

“…3 were tested for their ability to colonize A. thaliana roots in the presence of a synthetic, soil-derived community(Lozano- Andrade et al, 2021). This community comprises four bacterial species, Pedobacter sp., Rhodococcus globerulus, Stenotrophomas indicatrix and Chryseobacterium sp.…”

Genome analysis for the identification of genes involved in phenanthrene biodegradation pathway in Stenotrophomonas indicatrix CPHE1. Phenanthrene mineralization in soils assisted by integrated approaches

“…However, as far as we know, the study of Lara-Moreno et al (2021) was the first which demonstrated the ability of S. indicatrix to degrade PHE in solution. Stenotrophomonas indicatrix is a Gram-negative species that can be isolated from sunflower roots ( Adeleke et al, 2021 ), soils ( Lara-Moreno et al, 2021 ; Lozano-Andrade et al, 2021 ), oligotrophic water ponds ( Friedrich et al, 2021 ), or from food-associated sources ( Weber et al, 2018 ).…”