Complete Genome Sequences of Five
            <i>Burkholderia</i>
            Strains with Biocontrol Activity against Various Lettuce Pathogens

Biessy, Adrien; Ciotola, Marie; Cadieux, Mélanie; Albert, Daphné; Filion, Martin

doi:10.1128/mra.01120-21

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…The researchers showed that siderophore, indole acetic acid, hydrogen cyanide, salicylic acid production and E. clocae strain PS14 had the highest effect on controlling the agent both in vitro and in vivo. Similarly, as in this study, Burkholderia cepecia had biocontrol activity against various lettuce pathogens including S. sclerotiorum and Burkholderia strains harbor for the biosynthesis of pyrrolnitrin, burkholdins and cepacin (Biessy et al, 2022). In this study, rhizobacteria also increased plant growth.…”

Section: Discussionsupporting

confidence: 77%

The Use of Rhizobacteria on White Rot Disease and Growth of Lettuce

AKGÜL,

BELGÜZAR

2024

Black Sea Journal of Agriculture

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White rot caused by Sclerotinia sclerotiorum [(Lib.) de Bary] is one of the most important diseases negatively affecting lettuce production. In this study, effects of rhizobacteria containing different species on S. sclerotiorum were investigated. Also effect of rhizobacteria were determined growth of lettuce. Eight rhizobacteria strains (Enterobacter cloacae, E. aerogenes, Bacillus cereus, Microbacterium testaceum, Pseudomonas putida, P. chlororaphis, Acinetobacter calcoaceticus, and Burkholderia cepacia) were used in the study. Firstly, in vitro effects of rhizobacteria strains were investigated on the mycelial growth and sclerotia viability of S. sclerotiorum. Then, pot experiments were carried out under controlled greenhouse conditions to determine the effect of selected strains on white rot disease and growth of lettuce. The effect of tested bacteria on the mycelium growth of S. sclerotiorum ranged between 38.09-79.84%, and the P. putida strain had the highest impact. The bacterial strains were also effective on the sclerotia viability of S. sclerotiorum. The efficiency in pot experiment was between 50-90% on white rot, and the highest effect was recorded in A. calcoaceticus strain. In pot experiment rhizobacteria also increased plant growth. In particular, E. aerogenes was the most successful strain in plant growth. The results revealed that bacterial strains have different inhibitory effects in in vitro and in vivo experiments, while have the potential in the biological control of white rot disease and positive results on lettuce growth.

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Section: Discussionsupporting

confidence: 77%

The Use of Rhizobacteria on White Rot Disease and Growth of Lettuce

AKGÜL,

BELGÜZAR

2024

Black Sea Journal of Agriculture

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“…Genomic DNA libraries were prepared using PacBio SMRTbell Express Template Prep kit (Pacific Biosciences, Menlo Park, CA, USA) with the Pseudomonas strains displaying the strongest inhibitory activity against S. sclerotiorum . Genome sequencing was performed at the Integrated Microbiome Resource (Halifax, NS, Canada) using a PacBio Sequel sequencer (v3 chemistry) as described in Biessy et al (2022) . The genomes were assembled using the Flye v2.8.1 long-read assembler ( Kolmogorov et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Identification and genomic characterization of Pseudomonas spp. displaying biocontrol activity against Sclerotinia sclerotiorum in lettuce

Albert,

Zboralski,

Ciotola

et al. 2024

Front. Microbiol.

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Lettuce is an economically major leafy vegetable that is affected by numerous diseases. One of the most devastating diseases of lettuce is white mold caused by Sclerotinia sclerotiorum. Control methods for this fungus are limited due to the development of genetic resistance to commonly used fungicides, the large number of hosts and the long-term survival of sclerotia in soil. To elaborate a new and more sustainable approach to contain this pathogen, 1,210 Pseudomonas strains previously isolated from agricultural soils in Canada were screened for their antagonistic activity against S. sclerotiorum. Nine Pseudomonas strains showed strong in vitro inhibition in dual-culture confrontational assays. Whole genome sequencing of these strains revealed their affiliation with four phylogenomic subgroups within the Pseudomonas fluorescens group, namely Pseudomonas corrugata, Pseudomonas asplenii, Pseudomonas mandelii, and Pseudomonas protegens. The antagonistic strains harbor several genes and gene clusters involved in the production of secondary metabolites, including mycin-type and peptin-type lipopeptides, and antibiotics such as brabantamide, which may be involved in the inhibitory activity observed against S. sclerotiorum. Three strains also demonstrated significant in planta biocontrol abilities against the pathogen when either inoculated on lettuce leaves or in the growing substrate of lettuce plants grown in pots. They however did not impact S. sclerotiorum populations in the rhizosphere, suggesting that they protect lettuce plants by altering the fitness and the virulence of the pathogen rather than by directly impeding its growth. These results mark a step forward in the development of biocontrol products against S. sclerotiorum.

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“…Two Chryseobacterium strains (B21-013 and B21-037) were isolated from agricultural fields located in the Montérégie region (Quebec, Canada) as part of a search for plant-beneficial bacteria able to suppress various lettuce bacterial pathogens. These two strains were isolated as previously described ( 9 ). Briefly, 10 soil samples were collected as close as possible to the plant roots of various vegetable crop species grown on two agricultural farms located in Dunham and Brigham (Quebec, Canada).…”

Section: Announcementmentioning

confidence: 99%

Genome Sequences of Two Plant-Beneficial Chryseobacterium Strains Isolated from Agricultural Soils in the Province of Quebec, Canada

Biessy

Ciotola

Cadieux

et al. 2023

Microbiol Resour Announc

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Two Chryseobacterium strains, B21-013 and B21-037, were isolated from agricultural soils located in the province of Quebec, Canada, as part of a screening for plant-beneficial bacteria able to suppress Xanthomonas hortorum pv. vitians and other lettuce bacterial pathogens. Here, we report the genome sequences of these two organisms.

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Complete Genome Sequences of Five Burkholderia Strains with Biocontrol Activity against Various Lettuce Pathogens

Cited by 5 publications

References 14 publications

The Use of Rhizobacteria on White Rot Disease and Growth of Lettuce

The Use of Rhizobacteria on White Rot Disease and Growth of Lettuce

Identification and genomic characterization of Pseudomonas spp. displaying biocontrol activity against Sclerotinia sclerotiorum in lettuce

Genome Sequences of Two Plant-Beneficial Chryseobacterium Strains Isolated from Agricultural Soils in the Province of Quebec, Canada

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