Complete Genome Sequences of Dengue Virus Type 2 Epidemic Strains from Reunion Island and the Seychelles

Pascalis, Hervé; Biscornet, Leon; Toty, Céline; Hafsia, Sarah; Roche, Marjolaine; Desprès, Philippe; Nten, Célestine Atyame; Bibi, Jastin; Louange, Meggy; Gédéon, Jude; Mavingui, Patrick

doi:10.1128/mra.01443-19

Cited by 9 publications

(10 citation statements)

References 8 publications

(6 reference statements)

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“…Of note, in 2015 to 2016, 3 different serotypes (DENV-1, DENV-2, and DENV-3) were identified in 3 travelers returning from Indonesia and Malaysia [ 44 ], highlighting the diversity of DENV variants regularly introduced to the island. Also, comparison of the complete DENV-2 genomes from Reunion (2018) and Seychelles (2016) revealed 99.8% identity [ 48 ], suggesting that these DENV strains may have circulated between the SWIO islands.…”

Section: Resultsmentioning

confidence: 99%

Overview of dengue outbreaks in the southwestern Indian Ocean and analysis of factors involved in the shift toward endemicity in Reunion Island: A systematic review

et al. 2022

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Background Dengue is the world’s most prevalent mosquito-borne viral disease. It is endemic in many tropical and subtropical countries and represents a significant global health burden. The first reports of dengue virus (DENV) circulation in the South West Indian Ocean (SWIO) islands date back to the early 1940s; however, an increase in DENV circulation has been reported in the SWIO in recent years. The aim of this review is to trace the history of DENV in the SWIO islands using available records from the Comoros, Madagascar, Mauritius, Mayotte, Seychelles, and Reunion. We focus in particular on the most extensive data from Reunion Island, highlighting factors that may explain the observed increasing incidence, and the potential shift from one-off outbreaks to endemic dengue transmission. Methods Following the PRISMA guidelines, the literature review focused queried different databases using the keywords “dengue” or “Aedes albopictus” combined with each of the following SWIO islands the Comoros, Madagascar, Mauritius, Mayotte, Seychelles, and Reunion. We also compiled case report data for dengue in Mayotte and Reunion in collaboration with the regional public health agencies in these French territories. References and data were discarded when original sources were not identified. We examined reports of climatic, anthropogenic, and mosquito-related factors that may influence the maintenance of dengue transmission independently of case importation linked to travel. Findings and conclusions The first report of dengue circulation in the SWIO was documented in 1943 in the Comoros. Then not until an outbreak in 1976 to 1977 that affected approximately 80% of the population of the Seychelles. DENV was also reported in 1977 to 1978 in Reunion with an estimate of nearly 30% of the population infected. In the following 40-year period, DENV circulation was qualified as interepidemic with sporadic cases. However, in recent years, the region has experienced uninterrupted DENV transmission at elevated incidence. Since 2017, Reunion witnessed the cocirculation of 3 serotypes (DENV-1, DENV-2 and DENV-3) and an increased number of cases with severe forms and deaths. Reinforced molecular and serological identification of DENV serotypes and genotypes circulating in the SWIO as well as vector control strategies is necessary to protect exposed human populations and limit the spread of dengue.

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Section: Resultsmentioning

confidence: 99%

Overview of dengue outbreaks in the southwestern Indian Ocean and analysis of factors involved in the shift toward endemicity in Reunion Island: A systematic review

et al. 2022

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“…DENV-2 strain RUNJul (also named RUN-18) was isolated from an autochthonous patient diagnosed with mild dengue disease in La Reunion in 2018 [ 1 ]. The sequencing of RUN-18 revealed a close phylogenetic relationship with isolates of the DENV-2 cosmopolitan genotype, which were identified in Seychelles in 2016 [ 1 , 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…Very little is still known about the biological characteristics of the DENV-2 strains circulating in SWIO. The phylogenetic analysis showed that the DENV-2 isolates from La Reunion patients diagnosed for dengue disease in 2018 (Reunion/2018 strains), including the prototypical RUJul strain used in this study (GenBank accession number MN272404), belong to the cosmopolitan 1 lineage and, more precisely, to the C1-B sub-lineage [ 1 , 11 ]. The Reunion/2018 DENV-2 sequences also clustered with those identified in 2016 in Seychelles.…”

Section: Introductionmentioning

confidence: 99%

“…Conversely, the DENV-2 strain D2-K2_RIJ059/Dar es Salaam 2014 (GenBank accession number MG189962) that was isolated from a patient with dengue fever during an outbreak in Dar es Salaam in 2014 (Tanzania; TNZ) belongs to the cosmopolitan C2 sub-lineage [ 12 ]. This active co-circulation of the DENV-2 cosmopolitan lineages in SWIO is likely in relation to earlier introductions from India, Pakistan or China [ 1 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Instability of the NS1 Glycoprotein from La Reunion 2018 Dengue 2 Virus (Cosmopolitan-1 Genotype) in Huh7 Cells Is Due to Lysine Residues on Positions 272 and 324

Ogire

Diaz

Vidalain

et al. 2021

IJMS

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La Reunion island in the South West Indian Ocean is now endemic for dengue following the introduction of dengue virus serotype 2 (DENV-2) cosmopolitan-I genotype in 2017. DENV-2 infection causes a wide spectrum of clinical manifestations ranging from flu-like disease to severe dengue. The nonstructural glycoprotein 1 (NS1) has been identified as playing a key role in dengue disease severity. The intracellular NS1 exists as a homodimer, whereas a fraction is driven towards the plasma membrane or released as a soluble hexameric protein. Here, we characterized the NS1 glycoproteins from clinical isolates DES-14 and RUN-18 that were collected during the DENV-2 epidemics in Tanzania in 2014 and La Reunion island in 2018, respectively. In relation to hepatotropism of the DENV, expression of recombinant DES-14 NS1 and RUN-18 NS1 glycoproteins was compared in human hepatoma Huh7 cells. We observed that RUN-18 NS1 was poorly stable in Huh7 cells compared to DES-14 NS1. The instability of RUN-18 NS1 leading to a low level of NS1 secretion mostly relates to lysine residues on positions 272 and 324. Our data raise the issue of the consequences of a defect in NS1 stability in human hepatocytes in relation to the major role of NS1 in the pathogenesis of the DENV-2 infection.

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“…Dengue, like all flaviviruses, is a single-stranded positive sense RNA virus with a genome of about 11 kb, with a 5’-type I cap but without a polyadenylated tail (Colavita et al, 2020; Dang et al, 2020; Kamau et al, 2019; Pascalis et al, 2020; Selisko et al, 2014). The viral genome encodes a long polyprotein which is subsequently processed by both host and viral proteases to generate 10 mature viral proteins.…”

Section: Introductionmentioning

confidence: 99%

Structure of the Dengue Virus RNA Promoter

Sun

Varani

2022

Preprint

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Dengue virus, a single-stranded positive sense RNA virus, is the most prevalent mosquito-borne pathogen in the world. Like all RNA viruses, it uses conserved structural elements within its genome to control essential replicative steps. A 70 nucleotides stem-loop RNA structure (called SLA) found at the 5'-end of the genome of all flaviviruses, functions as the promoter for viral replication. This highly conserved structure interacts with the viral polymerase NS5 to initiate RNA synthesis. Here we report the NMR structure of a monomeric SLA from Dengue virus serotype 1, assembled to high-resolution from independently folded structural elements. The DENV1 SLA has an L-shape structure, where the top and side helices are coaxially-stacked and the bottom helix is roughly perpendicular to them. Because the sequence is highly conserved among different flavivirus genomes, it is likely that the three-dimensional fold and local structure of SLA are also conserved among flaviviruses and required for efficient replication. This work provides structural insight into the Dengue promoter and provides the foundation for the discovery of new antiviral drugs that target this essential replicative step.

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Complete Genome Sequences of Dengue Virus Type 2 Epidemic Strains from Reunion Island and the Seychelles

Cited by 9 publications

References 8 publications

Overview of dengue outbreaks in the southwestern Indian Ocean and analysis of factors involved in the shift toward endemicity in Reunion Island: A systematic review

Overview of dengue outbreaks in the southwestern Indian Ocean and analysis of factors involved in the shift toward endemicity in Reunion Island: A systematic review

Instability of the NS1 Glycoprotein from La Reunion 2018 Dengue 2 Virus (Cosmopolitan-1 Genotype) in Huh7 Cells Is Due to Lysine Residues on Positions 272 and 324

Structure of the Dengue Virus RNA Promoter

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