Complete Genome Sequences of a Cytopathic/Noncytopathic Pair of Border Disease Viruses

Neill, John D.; Ridpath, Julia F.

doi:10.1128/genomea.00260-14

Cited by 3 publications

(2 citation statements)

References 11 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10,22 As their names suggest, BVDV-1 and BVDV-2 were first isolated from cattle and are recognized as economically significant pathogens in both the dairy and beef industries. 1,13 A third classic pestivirus species, BDV, has been described in the United States, 17 although information regarding prevalence is scarce. Although sheep are the primary host of BDV infection, cattle infection has been described.…”

Section: Introductionmentioning

confidence: 99%

A serosurvey for ruminant pestivirus exposure conducted using cattle sera collected for brucellosis surveillance in the United States

2017

Self Cite

View full text Add to dashboard Cite

Four species of ruminant pestivirus are currently circulating in the United States: Bovine viral diarrhea virus 1 and 2 (BVDV-1, -2; predominant host: cattle), Border disease virus (BDV; predominant host: sheep), and pronghorn virus (sporadically detected in wild ruminants). A third bovine pestivirus called HoBi-like virus has been detected in cattle and water buffalo in South America, Asia, and Europe. To date, no isolations of HoBi-like viruses from U.S. cattle have been reported. To assess exposure, 2,000 cattle sera, collected between 2014 and 2015 as part of the U.S. brucellosis surveillance program, were tested for antibodies against BVDV-1, BVDV-2, and HoBi-like viruses. In addition, RNA was extracted and tested by reverse transcription-polymerase chain reaction for the presence of pestiviruses; all samples tested negative. The percent of VN-positive samples was 91.3% for BVDV-1, 89.3% for BVDV-2, and 84.9% for HoBi-like viruses. Because the 3 bovine pestiviruses are antigenically cross-reactive, the comparative level of antibody against each pestivirus species was determined. Based on comparative titers, samples were segregated into 6 categories: no titers (7.6%), titers clearly higher against BVDV-1 (22.2%), titers substantially higher against BVDV-2 (9.1%), BVDV-1 and BVDV-2 titers equivalent but substantially higher than HoBi titers (25.7%), titers substantially higher against HoBi-like viruses (0%), and equivocal (35.4%). Titers tended to be higher against BVDV-1 than BVDV-2. However, the overall percentage of animals with titers below levels considered protective against acute bovine pestivirus infection were ~11% for BVDV-1, 12% BVDV-2, and 18% for HoBi-like virus.

show abstract

Section: Introductionmentioning

confidence: 99%

A serosurvey for ruminant pestivirus exposure conducted using cattle sera collected for brucellosis surveillance in the United States

2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sequencing of the genomic RNA of pronghorn virus from the cell culture supernatant was done using the Ion Torrent ( 4 , 5 ) and Illumina MiSeq ( 6 , 7 ) sequencing protocols, as previously described. The 3′ end of the genomic RNA was confirmed by 3′ RACE using a primer set designed to amplify the 3′ terminal 600 bases.…”

Section: Genome Announcementmentioning

confidence: 99%