Complete Genome Sequence of Vibrio coralliilyticus Strain OCN014, Isolated from a Diseased Coral at Palmyra Atoll

Ushijima, Blake; Videau, Patrick; Poscablo, Donna; Vine, Veronica; Salcedo, M. T.; Aeby, Greta S.; Callahan, Sean M.

doi:10.1128/genomea.01318-14

Cited by 17 publications

(18 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The complete genome of isolate OCN014 was sequenced previously (Ushijima et al ., ), and sequencing data (CP009264, CP009265 and CP009266) was used to identify OCN014 as a strain of V. coralliilyticus . The 16S rRNA gene of OCN014 shared 99% nucleotide identity to the sequence from V. coralliilyticus strain OCN008 (KF042020), another experimentally verified coral pathogen (Ushijima et al ., ).…”

Section: Resultsmentioning

confidence: 99%

“…All primers used in this study are listed in Supporting Information Table S1. Sequences for the OCN014 16S rRNA gene and genes for MLSA were obtained from the publically available OCN014 genome sequence on NCBI (Ushijima et al ., ). polymerase chain reaction (PCR) amplification and sequencing of the 16S rRNA gene from other bacterial isolates was done as previously described (Ushijima et al ., 2014).…”

Section: Methodsmentioning

confidence: 97%

“…All bacterial strains used in this study are listed in Table . Vibrio coralliilyticus strain OCN014 was isolated from a diseased A. cytherea fragment as described previously (Ushijima et al ., ), and V. nereis strain OCN044 was isolated from healthy A. cytherea in the same manner. Marine bacteria were grown in GSW medium that was prepared by re‐suspending 4 g l −1 of tryptone, 2 g l −1 of yeast extract and 2 ml l −1 of glycerol in a litre of seawater that had been previously filtered through a 0.22 µm pore membrane filter, and autoclaving the mixture before use.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Mutation of the toxR or mshA genes from Vibrio coralliilyticus strain OCN014 reduces infection of the coral Acropora cytherea

Ushijima

Videau

Poscablo

et al. 2016

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

Thermal stress increases the incidence of coral disease, which is predicted to become more common with climate change, even on pristine reefs such as those surrounding Palmyra Atoll in the Northern Line Islands that experience minimal anthropogenic stress. Here we describe a strain of Vibrio coralliilyticus, OCN014, which was isolated from Acropora cytherea during an outbreak of Acropora white syndrome (AWS), a tissue loss disease that infected 25% of the A. cytherea population at Palmyra Atoll in 2009. OCN014 recreated signs of disease in experimentally infected corals in a temperature-dependent manner. Genes in OCN014 with expression levels positively correlated with temperature were identified using a transposon-mediated genetic screen. Mutant strains harbouring transposon insertions in two such genes, toxR (a toxin regulator) and mshA (the 11th gene of the 16-gene mannose-sensitive hemagglutinin (MSHA) type IV pilus operon), had reduced infectivity of A. cytherea. Deletion of toxR and the MSHA operon in a second strain of V. coralliilyticus, OCN008, that induces acute Montipora white syndrome in a temperature-independent manner had similarly reduced virulence. This work provides a link between temperature-dependent expression of virulence factors in a pathogen and infection of its coral host.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Mutation of the toxR or mshA genes from Vibrio coralliilyticus strain OCN014 reduces infection of the coral Acropora cytherea

Ushijima

Videau

Poscablo

et al. 2016

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…To compare genome similarities with other V. coralliilyticus strains, average nucleotide identities (ANI) were calculated using Enviomics (11). ANI values for each strain with V. coralliilyticus strains ATCC BAA-450 (12), RE98 (13), OCN014 (14), and 58 (15) were >96%, corresponding to grouping at the species level (16, 17). All three strains had open reading frames displaying 99% homology to the VtpA extracellular zinc metalloprotease of V. coralliilyticus RE98, a known virulence factor for C. gigas (18).…”

Section: Genome Announcementmentioning

confidence: 99%

Three Draft Genome Sequences of Vibrio coralliilyticus Strains Isolated from Bivalve Hatcheries

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Currently, complete genomes of V. coralliilyticus OCN014 [24], RE98 [25], RE22 [26], and 58 [27], and V. tubiashi ATCC 19109 [28] are available in the GenBank database. Therefore, the OrthoANI algorithm [29] was applied to assess the overall genome similarities between V. coralliilyticus SNUTY-1 and the other related Vibrio strains.…”

Section: Genome Of V Coralliilyticus Snuty-1mentioning

confidence: 99%

Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae

Kim

Jun²,

Giri

et al. 2020

Pathogens

View full text Add to dashboard Cite

Vibrio coralliilyticus is known as a coral pathogen that also infects marine bivalve larvae worldwide. It is considered to be one of the major constraints in artificial marine bivalve seed production as it causes mortality. In this study, we first isolated and characterized a high virulent of V. coralliilyticus designated as SNUTY-1 that was the cause of Pacific oyster larvae mortality in Korea. In the pathogenicity test, exposure to 2.14 × 105 CFU/mL for 24 h caused mortality to 88.65 ± 2.4% of the tested healthy Pacific oyster larvae. SNUTY-1 showed anti-microbial resistance to β-lactams, such as penicillins, cephalosporins, and carbapenems. We sequenced and assembled the complete genome of SNUTY-1 (5,842,676 bp), consisting of two chromosomes (Chr I and Chr II) and two plasmids (pSNUTY1 and pSNUTY2). The COG functional analysis confirmed that Chr I had more genes associated with basic cellular functions in comparison to Chr II. The results of the phylogenetic trees based on OrthoANI values indicated that the SNUTY-1 was closely related to V. coralliilyticus strains. SNUTY-1 had a unique plasmid (pSNUTY2), which could mean that the Korean isolate is different from other sequenced V. coralliilyticus strains from different geographical origins. Toxic proteins such as cytolysin/hemolysin and extracellular metalloprotease genes were encoded on Chr I and Chr II of SNUTY-1. These data facilitate the control of V. coralliilyticus infections in aquaculture by providing valuable insights into the biodiversity of this organism and valuable information for the study of virulence factors.

show abstract

Complete Genome Sequence of Vibrio coralliilyticus Strain OCN014, Isolated from a Diseased Coral at Palmyra Atoll

Cited by 17 publications

References 14 publications

Mutation of the toxR or mshA genes from Vibrio coralliilyticus strain OCN014 reduces infection of the coral Acropora cytherea

Mutation of the toxR or mshA genes from Vibrio coralliilyticus strain OCN014 reduces infection of the coral Acropora cytherea

Three Draft Genome Sequences of Vibrio coralliilyticus Strains Isolated from Bivalve Hatcheries

Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae

Contact Info

Product

Resources

About