Complete Genome Sequence of Vibrio mediterranei 117-T6, a Potentially Pathogenic Bacterium Isolated from the Conchocelis of Pyropia spp

Liu, Qiqin; Xu, Mengya; He, Yingyun; Zhang, Tao; Yang, Rui; Chen, Haimin

doi:10.1128/mra.01569-18

Cited by 9 publications

(4 citation statements)

References 11 publications

(11 reference statements)

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“…For Vibrio species, we also analysed the genome location of these T3SS2 gene clusters. In addition to the described chromosomal location of T3SS2 within VPAI-7 of V. parahaemolyticus RIMD2210633 [ 4 ], we identified a T3SS2 gene cluster within the plasmid of a recently sequenced Vibrio mediterranei strain 117-T6 (CP033579) [ 77 ] ( Fig. 5a ).…”

Section: Resultsmentioning

confidence: 99%

“…In addition to the described chromosomal location of T3SS2 within VPAI-7 of V. parahaemolyticus RIMD2210633 [4], we identified a T3SS2 gene cluster within the plasmid of a recently sequenced Vibrio mediterranei strain 117-T6 (CP033579) [77] (). Another interesting observation was the presence of a T3SS2 gene cluster inserted right next to a T3SS1 gene cluster in Vibrio campbellii strain UMTGB204.…”

Section: Resultsmentioning

confidence: 99%

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Vibrio type III secretion system 2 is not restricted to the Vibrionaceae and encodes differentially distributed repertoires of effector proteins

et al. 2023

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Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis worldwide. A distinctive feature of the O3:K6 pandemic clone, and its derivatives, is the presence of a second, phylogenetically distinct, type III secretion system (T3SS2) encoded within the genomic island VPaI-7. The T3SS2 allows the delivery of effector proteins directly into the cytosol of infected eukaryotic cells to subvert key host-cell processes, critical for V. parahaemolyticus to colonize and cause disease. Furthermore, the T3SS2 also increases the environmental fitness of V. parahaemolyticus in its interaction with bacterivorous protists; hence, it has been proposed that it contributed to the global oceanic spread of the pandemic clone. Several reports have identified T3SS2-related genes in Vibrio and non- Vibrio species, suggesting that the T3SS2 gene cluster is not restricted to the Vibrionaceae and can mobilize through horizontal gene transfer events. In this work, we performed a large-scale genomic analysis to determine the phylogenetic distribution of the T3SS2 gene cluster and its repertoire of effector proteins. We identified putative T3SS2 gene clusters in 1130 bacterial genomes from 8 bacterial genera, 5 bacterial families and 47 bacterial species. A hierarchical clustering analysis allowed us to define six T3SS2 subgroups (I–VI) with different repertoires of effector proteins, redefining the concepts of T3SS2 core and accessory effector proteins. Finally, we identified a subset of the T3SS2 gene clusters (subgroup VI) that lacks most T3SS2 effector proteins described to date and provided a list of 10 novel effector candidates for this subgroup through bioinformatic analysis. Collectively, our findings indicate that the T3SS2 extends beyond the family Vibrionaceae and suggest that different effector protein repertories could have a differential impact on the pathogenic potential and environmental fitness of each bacterium that has acquired the Vibrio T3SS2 gene cluster.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Vibrio type III secretion system 2 is not restricted to the Vibrionaceae and encodes differentially distributed repertoires of effector proteins

et al. 2023

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“…In terms of genome location, in addition to the described chromosomal location of T3SS2 within VPAI-7 of V. parahamolyticus RIMD2210633 [4], we identified a T3SS2 gene cluster within the plasmid of a recently sequenced V. meditteraniae strain 117-T6 (CP033579) [74] ( Fig. 4A ).…”

Section: Resultsmentioning

confidence: 99%

TheVibrioType III Secretion System 2 is not restricted to theVibrionaceaeand encodes differentially distributed repertoires of effector proteins

Jerez

Plaza

Bravo

et al. 2022

Preprint

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Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis worldwide. A distinctive feature of the O3:K6 pandemic clone, and its derivatives, is the presence of a second, phylogenetically distinct, Type III Secretion System (T3SS2) encoded within the genomic island VPaI-7. The T3SS2 allows the delivery of effector proteins directly into the cytosol of infected eukaryotic cells to subvert key host cell processes, critical for V. parahaemolyticus to colonize and cause disease. Furthermore, the T3SS2 also increases the environmental fitness of V. parahaemolyticus in its interaction with bacterivorous protists; hence it has been proposed that it contributed to the global oceanic spread of the pandemic clone. Several reports have identified T3SS2-related genes in Vibrio and non-Vibrio species, suggesting that the T3SS2 gene cluster is not restricted to the Vibrionaceae and can mobilize through horizontal gene transfer events. In this work, we performed a large-scale genomic analysis to determine the phylogenetic distribution of the T3SS2 gene cluster and its repertoire of effector proteins. We identified putative T3SS2 gene clusters in 1130 bacterial genomes from eight bacterial genera and five bacterial families. A hierarchical clustering analysis allowed us to define six T3SS2 subgroups (I-VI) with different repertoires of T3SS2 effector proteins, redefining the concepts of T3SS2 core and accessory effector proteins. Finally, we identified a subset of T3SS2 gene clusters that lack most T3SS2 effector proteins described to date and provided a list of 10 novel effector candidates for this subgroup through bioinformatic analysis. Collectively, our findings indicate that the T3SS2 extends beyond the Vibrionaceae family and suggest that different effector protein repertories could have a differential impact on the pathogenic potential and environmental fitness of all the bacteria that have acquired the Vibrio T3SS2 gene cluster.

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“…It is considered that Pseudomonas, Vibrio or some other bacteria can infect the injured parts of algae, proliferate, and then secrete some degrading material to destroy algal tissue and cause YSD (Wang et al, 2011). Among them, Vibrio mediterranei 117-T6 (Vm 117-T6) has been proved to be a pathogenic bacterium that can cause YSD by Koch's postulates, which can loosen the cellular structures and degrade the contents of Neoporphyra haitanensis (Pyropia haitanensis, before 2020) conchocelis, and even its cells are completely bleached (Liu et al, 2019. It has also been reported that YSD may be caused by the high temperature or continuous rainfall that raises the river water level and carries more organic matter into the seashore (Wang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Using oligoagar to improve the survival of Neoporphyra haitanensis conchocelis infected by Vibrio mediterranei 117-T6

Mao

Yang

et al. 2023

J Appl Phycol

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Neoporphyra haitanensis is a vital economic seaweed in China. As an infectious disease with a high incidence in the conchocelis sporeling culture of Porphyra, yellow spot disease (YSD) seriously affects the industrial development of seaweed cultivation. In the present study, we analyzed the protective effect of oligoagar (OA) on YSD caused by Vibrio mediterranei 117-T6 (Vm 117-T6). The experiment was conducted under the standard culture conditions of N. haitanensis conchocelis. The results showed that OA treatment (0.1‰ OA treatment for 1 h and performed twice with a 48 h interval) could signi cantly improve the survival rate of N. haitanensis conchocelis infected with Vm 117-T6 (P < 0.05). Meanwhile, OA treatment could stabilize the physiological and biochemical indexes, such as superoxide dismutase (SOD), phycobiliprotein and photosynthetic pigment content of Porphyra. Therefore, OA treatment had good application potential for preventing YSD caused by Vm 117-T6.

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Complete Genome Sequence of Vibrio mediterranei 117-T6, a Potentially Pathogenic Bacterium Isolated from the Conchocelis of Pyropia spp

Abstract: Vibrio mediterranei is a Gram-negative bacterium of the family Vibrionaceae. Vibrio mediterranei strain 117-T6 was pathogenic to Pyropia yezoensis, a red seaweed cultivated in China, by causing death to its conchocelis.

Cited by 9 publications

References 11 publications

Vibrio type III secretion system 2 is not restricted to the Vibrionaceae and encodes differentially distributed repertoires of effector proteins

Vibrio type III secretion system 2 is not restricted to the Vibrionaceae and encodes differentially distributed repertoires of effector proteins

TheVibrioType III Secretion System 2 is not restricted to theVibrionaceaeand encodes differentially distributed repertoires of effector proteins

Using oligoagar to improve the survival of Neoporphyra haitanensis conchocelis infected by Vibrio mediterranei 117-T6

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