Complete Genome Sequence of the Cellulolytic Thermophile Caldicellulosiruptor obsidiansis OB47 ^T

Abstract

Caldicellulosiruptor obsidiansis OB47T (ATCC BAA-2073, JCM 16842) is an extremely thermophilic, anaerobic bacterium capable of hydrolyzing plant-derived polymers through the expression of multidomain/multifunctional hydrolases. The complete genome sequence reveals a diverse set of carbohydrate-active enzymes and provides further insight into lignocellulosic biomass hydrolysis at high temperatures.

Genome Sequence of the Mercury-Methylating and Pleomorphic Desulfovibrio africanus Strain Walvis Bay

“…One region has 100 nucleotides in the sequence that are not identified. The order and arrangement of known sequences are correct based on paired-end sequence data and the finishing process, as described earlier (15). Plasmid DNA was not detected in this strain.…”

“…All aspects of the DNA sequencing and genome finishing for this project have been described previously (15). The genome size is 4,200,509 bp, and the final assembly is based on ϳ85 Mb of 454 draft data, which provide an average of ϳ20-fold coverage of the genome, and ϳ5,363 Mb of Illumina draft data, which provide an average of 1,247-fold coverage of the genome.…”

Complete Genome Sequences for the Anaerobic, Extremely Thermophilic Plant Biomass-Degrading Bacteria Caldicellulosiruptor hydrothermalis , Caldicellulosiruptor kristjanssonii , Caldicellulosiruptor kronotskyensis , Caldicellulosiruptor owensensis , and Caldicellulosiruptor lactoaceticus

“…While 16S rRNA phylogeny indicates a very close relationship within the eight species studied thus far (94.8% to 99.4% identity), microbiological analysis indicated that the genus is more physiologically divergent than previously thought (2). Three complete genome sequences are currently available for this genus, those of C. saccharolyticus (19), C. bescii (12), and C. obsidiansis (5), all of which are capable of crystalline cellulose hydrolysis. To further probe the plant biomass-degrading capacity among Caldicellulosiruptor species, genome sequences of five additional members of this genus, including some hemicellulolytic but less cellulolytic members, were completed: those of C. hydrothermalis, C. kristjanssonii, C. kronotskyensis, C. owensensis, and C. lactoaceticus (draft phase).…”

“…All general aspects of library construction and sequencing can be found at http://www.jgi.doe.gov/. The genomes of Caldicellulosiruptor species were sequenced at the U.S. Department of Energy Joint Genome Institute (JGI) using a combination of Illumina (1) and 454 (14) technologies similar to the sequencing strategy for C. obsidiansis (5). Sequencing data were assembled either with VELVET (21) or by being converted into a Phrap assembly.…”

Draft Genome Sequence of Rhizobium sp. Strain PDO1-076, a Bacterium Isolated from Populus deltoides

“…Draft genome sequence data were generated using a combination of 454 (7) and Illumina (2) technologies essentially as described previously (3). The 454 FLX shotgun data consisted of 272,469 reads and generated 56,371,663 bp.…”