Complete Genome Sequence of the Attenuated Corynebacterium pseudotuberculosis Strain T1

Almeida, Síntia; Loureiro, Dan; Portela, Ricardo Wagner; Mariano, Diego; Sousa, Thiago J.; Pereira, Felipe Luiz; Dorella, Fernanda Alves; Carvalho, Alex F.; Moura-Costa, Lília Ferreira de; Leal, Carlos Augusto Gomes; Figueiredo, Henrique César Pereira; Meyer, Roberto; Azevedo, Vasco

doi:10.1128/genomea.00947-16

Cited by 5 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…57 T1Ion PGM 200 bp1,118,022Newbler v. 2.92.337295.93CP015100.1Almeida et al . 58 MB11Ion PGM 200 bp6,753,458Mira 4.0.22.3634299.24CP013260.1Baraúna et al . 59 …”

Section: Introductionmentioning

confidence: 99%

Re-sequencing and optical mapping reveals misassemblies and real inversions on Corynebacterium pseudotuberculosis genomes

Sousa

Parise

Profeta

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

The number of draft genomes deposited in Genbank from the National Center for Biotechnology Information (NCBI) is higher than the complete ones. Draft genomes are assemblies that contain fragments of misassembled regions (gaps). Such draft genomes present a hindrance to the complete understanding of the biology and evolution of the organism since they lack genomic information. To overcome this problem, strategies to improve the assembly process are developed continuously. Also, the greatest challenge to the assembly progress is the presence of repetitive DNA regions. This article highlights the use of optical mapping, to detect and correct assembly errors in Corynebacterium pseudotuberculosis. We also demonstrate that choosing a reference genome should be done with caution to avoid assembly errors and loss of genetic information.

show abstract

“…57 T1Ion PGM 200 bp1,118,022Newbler v. 2.92.337295.93CP015100.1Almeida et al . 58 MB11Ion PGM 200 bp6,753,458Mira 4.0.22.3634299.24CP013260.1Baraúna et al . 59 …”

Section: Introductionmentioning

confidence: 99%

Re-sequencing and optical mapping reveals misassemblies and real inversions on Corynebacterium pseudotuberculosis genomes

Sousa

Parise

Profeta

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The following are available online at , Table S1: Corynebacterium pseudotuberculosis strains examined in the in silico sequence analysis; Table S2: General information on the genomes; Figure S1: Pairwise differences in the distance matrix of six whole-genome-sequenced isolates belonging to three different STs. References [ 59 , 60 , 61 , 62 , 63 , 64 ] are cited in the supplementary materials.…”

mentioning

confidence: 99%

Molecular Characterization of Corynebacterium pseudotuberculosis Isolated over a 15-Year Period in Switzerland

Schlicher

Schmitt

Stevens

et al. 2021

Veterinary Sciences

View full text Add to dashboard Cite

Corynebacteriumpseudotuberculosis biovar Ovis is the etiological agent of the contagious and chronic disease caseous lymphadenitis (CLA) in sheep and goats. The economic impact of CLA in Switzerland remains largely unknown, and the transmission modalities, as well as the genetic diversity of circulating strains, are poorly understood. This work presents further characterization data for 215 C. pseudotuberculosis isolates from sheep, goats and a dromedary originating from Switzerland and the Principality of Liechtenstein, collected over a 15-year period. The isolates were classified into the two biovars Ovis and Equi, analyzed for the presence of the diphtheria-like toxin gene and characterized using MLSA. All sheep and goat isolates were classified as C. pseudotuberculosis biovar Ovis. The isolate from a dromedary was classified as biovar Equi. No isolates harboring the diphtheria-like toxin gene were detected. Phylogenetic analysis of the concatenated sequences of four genes revealed the existence of 24 clusters. There was no correlation between MLSA sequence types, year of isolation and the geographical origin of the isolates. These findings confirm the presence of several MLSA sequence types in the study area and over a 15-year period. Moreover, no sheep- and goat-specific MLSA sequence types were found.

show abstract

“…The second strain, which was identified in the same way as the T1 strain, was isolated from a goat and called VD57, presenting high virulence for mice and goats, inducing the development of severe clinical symptoms of CLA [12,13]. These two strains belong to the biovar ovis and were chosen because they are the C. pseudotuberculosis strains that have their virulence profile well characterized in mice and in small ruminants, and have their genome already sequenced [14,15]. Additional data are given in Online Resource 1.…”

Section: Pseudotuberculosis Strainsmentioning

confidence: 99%

Cell wall glycolipids from Corynebacterium pseudotuberculosis strains with different virulences differ in terms of composition and immune recognition

et al. 2020

Self Cite

View full text Add to dashboard Cite

Caseous lymphadenitis (CLA) is an infectious disease caused by Corynebacterium pseudotuberculosis in small ruminants and is characterized by the development of granulomas in the lymph nodes, spleen, liver, and lungs. Although little is known about the host-pathogen relationship of this bacterium, it was previously reported that the pathogen's lipids are important for its taxonomic classification and survival inside macrophages. However, there are no studies regarding the composition of these molecules. In this study, cell wall glycolipids from two C. pseudotuberculosis strains presenting different virulence profiles were purified and its composition was characterized. A difference was observed between the electrophoretic and chromatogram profiles for cell wall components from the two strains, mainly among molecules with low molecular weights. IgM from sheep with acute CLA recognized antigens with an estimated molecular weight of 11 kDa of the low-pathogenicity strain, while low-molecular weight antigens from the high-pathogenicity strain presented a lower recognition by these antibodies. Mass spectrometry analysis showed that the cell wall of the high-pathogenicity strain contained glycolipids with high amounts of unsaturated fatty acids and glycerophosphoinositols, which may contribute to the capacity of this strain to cause severe disease. In conclusion, it is indicated that cell wall non-protein antigens can play a key role in C. pseudotuberculosis virulence.

show abstract

Complete Genome Sequence of the Attenuated Corynebacterium pseudotuberculosis Strain T1

Cited by 5 publications

References 6 publications

Re-sequencing and optical mapping reveals misassemblies and real inversions on Corynebacterium pseudotuberculosis genomes

Re-sequencing and optical mapping reveals misassemblies and real inversions on Corynebacterium pseudotuberculosis genomes

Molecular Characterization of Corynebacterium pseudotuberculosis Isolated over a 15-Year Period in Switzerland

Cell wall glycolipids from Corynebacterium pseudotuberculosis strains with different virulences differ in terms of composition and immune recognition

Contact Info

Product

Resources

About