Complete genome sequence of the thermophilic Acidobacteria, Pyrinomonas methylaliphatogenes type strain K22T

Abstract

Strain K22T is the type species of the recently- described genus Pyrinomonas, in subdivision 4 of the phylum Acidobacteria (Int J Syst Evol Micr. 2014; 64(1):220–7). It was isolated from geothermally-heated soil from Mt. Ngauruhoe, New Zealand, using low-nutrient medium. P. methylaliphatogenes K22T has a chemoheterotrophic metabolism; it can hydrolyze a limited range of simple carbohydrates and polypeptides. Its cell membrane is dominated by iso-branching fatty acids, and up to 40 % of its lipid content is membrane-spanning and ether lipids. It is obligately aerobic, thermophilic, moderately acidophilic, and non-spore-forming. The 3,788,560 bp genome of P. methylaliphatogenes K22T has a G + C content of 59.36 % and contains 3,189 protein-encoding and 55 non-coding RNA genes. Genomic analysis was consistent with nutritional requirements; in particular, the identified transporter classes reflect the oligotrophic nature of this strain.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-015-0099-5) contains supplementary material, which is available to authorized users.

Brevitalea aridisoli, B. deliciosa and Arenimicrobium luteum, three novel species of Acidobacteria subdivision 4 (class Blastocatellia) isolated from savanna soil and description of the novel family Pyrinomonadaceae

“…Under aerobic conditions all three strains grew equally well to slightly better in the dark, under microaerobic conditions no growth was observed under any conditions. This is in congruence with the findings for P. methylaliphatogenes K22 T which, in contrast to the photoheterotroph C. thermophilum B T , lacks all indications of phototrophy (Crowe et al, 2014;Lee et al, 2015).…”

“…While the G+C contents for strains Ac_11_E3 T (55.9 mol%) and Ac_16_C4 T (54.7 mol %) were within the range reported for other described members of the class Blastocatellia (46.5-61.3 mol%; Pascual et al, 2015), the DNA G+C content of Ac_12_G8 T (66.9 mol%) was considerably higher (Table 1). The DNA G+C content of the next most closely related species P. methylaliphatogenes K22 T varied between 59.4 and 59.6 mol%, depending on the analysis method used (Crowe et al, 2014;Lee et al, 2015).…”

“…Ac_11_E3 T hydrolyzed cellulose and xylan, while Ac_12_G8 T and Ac_16_C4 T were able to degrade starch (Table 1). A genome analysis indicated the capability of P. methylaliphatogenes K22 T to degrade cellulose and xylan (Lee et al, 2015).…”

“…All strains utilized casamino acids, yeast extract and peptone. +, positive; À, negative; (+), weakly positive; ND, no data available; P, predicted from genome analysis (Lee et al, 2015). Only major fatty acids (>5 % of total fatty acid amount of at least one of the novel strains) are listed.…”

“…With the exception of Chloracidobacterium thermophilum (Bryant et al, 2007;Tank & Bryant, 2015) which was isolated from phototrophic microbial mats of a hot spring in Yellowstone National Park, USA, all members of the class Blastocatellia originated from terrestrial environments. Pyrinomonas methylaliphatogenes (Crowe et al, 2014;Lee et al, 2015) was obtained from a geothermally heated volcanic soil in New Zealand. Blastocatella fastidiosa (Foesel et al, 2013), Aridibacter famidurans and Aridibacter kavangonensis (Huber et al, 2014), Stenotrophobacter terrae, Stenotrophobacter roseus and Stenotrophobacter namibiensis (Pascual et al, 2015), as well as Tellurimicrobium multivorans (Pascual et al, 2015) were isolated from semiarid savanna soils in northern Namibia.…”