Complete genome sequence of the Pogostemon cablin bacterial wilt pathogen Ralstonia solanacearum strain SY1

Abstract

Background Ralstonia solanacearum causes bacterial wilt of Pogostemon cablin which is an important aromatic herb and also the main materials of COVID-19 therapeutic traditional drugs. However, we are lacking the information on the genomic sequences of R. solanacearum isolated from P. cablin . Objective The acquisition and analysis of this whole-genome sequence of the P. cablin bacterial wilt pathogen. Methods An R. solanacearum strain, named SY1, was isolated from infected P. cablin plants, and the complete genome sequence was sequenced and analyzed. Results The SY1 strain contains a 3.70-Mb chromosome and a 2.18-Mb megaplasmid, with GC contents of 67.57% and 67.41%, respectively. A total of 3308 predicted genes were located on the chromosome and 1657 genes were located in the megaplasmid. SY1 strain has 273 unique genes compared with five representative R. solanacearum strains, and these genes were enriched in the plant–pathogen interaction pathway. SY1 possessed a higher syntenic relationship with phylotype I strains, and the arsenal of type III effectors predicted in SY1 were also more closely related to those of phylotype I strains. SY1 contained 14 and 5 genomic islands in its chromosome and megaplasmid, respectively, and two prophage sequences in its chromosome. In addition, 215 and 130 genes were annotated as carbohydrate-active enzymes and antibiotic resistance genes, respectively. Conclusion This is the first genome-scale assembly and annotation for R. solanacearum which isolated from infected P. cablin plants. The arsenal of virulence and antibiotic resistance may as the determinants in SY1 for infection of P. cablin plants. Supplementary Information The online version contains supplementary material available at 10.1007/s13258-022-01270-9.

Comparative transcriptome analysis revealed molecular mechanisms of peanut leaves responding to Ralstonia solanacearum and its type III secretion system mutant

“…BW, caused by Ralstonia solanacearum , is a very destructive soil-borne bacterial disease. Ralstonia solanacearum , with a wide host range, can infect more than 200 plant species in 54 families, such as peanut, potato ( Solanum tuberosum ), ginger ( Zingiber officinale ), and patchouli ( Pogostemon cablin ; Jiang et al, 2017 ; Sun et al, 2022 ). The economic losses caused by BW are difficult to estimate every year in the world ( Genin, 2010 ; Deberdt et al, 2021 ).…”