Complete Genome Sequence of the Edwardsiella ictaluri-Specific Bacteriophage PEi21, Isolated from River Water in Japan

Yasuike, Motoshige; Wang, Kai; Nakamura, Yoji; Fujiwara, Atushi; Kawato, Yasuhiko; Hassan, Ebtsam Sayed; Mahmoud, Mahmoud Mostafa; Nagai, Satoshi; Kobayashi, Takanori; Ototake, Mitsuru; Nakai, Toshiko

doi:10.1128/genomea.00228-14

Cited by 12 publications

(14 citation statements)

References 17 publications

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“…2). Unlike other previously reported Edwardsiella phage genomes [3,[22][23][24], GF-2 possesses lysogeny-related genes such as integrase, repressor CI, regulatory protein CII and anti-repressors (Fig. 2).…”

Section: Rastmentioning

confidence: 70%

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Full-genome sequence of a novel myovirus, GF-2, infecting Edwardsiella tarda: comparison with other Edwardsiella myoviral genomes

et al. 2015

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Edwardsiellosis, which is caused by Edwardsiella tarda, a Gram-negative bacterium, is one of the most serious infectious diseases in both marine and freshwater fish farms worldwide. Previously, we reported the complete genome sequences of three E. tarda-lytic bacteriophages (two podoviruses and a myovirus), which were isolated from fish tissues and fish-rearing seawater. Further genomic information regarding E. tarda phages is important for understanding phage-host interactions as well as for applications of the phages for the control of disease. Here, we report the complete genome sequence of a novel E. tarda phage (GF-2) of myovirus morphology (family Myoviridae), isolated from tissue homogenates of a cultured Japanese flounder (Paralichthys olivaceus) that succumbed to edwardsiellosis in Japan. The size of the entire genome was 43,129 bp, with a GC content of 51.3 % and containing 82 open reading frames (ORFs). The GF-2 genome possesses lysogeny-related genes that have not been found in the reported Edwardsiella phage genomes. Comparative genomics of Edwardsiella myophages suggest that the C-terminal domains of the tail fiber proteins have relevance to their host specificity. Thus, GF-2 genome information provides a novel resource for our understanding of the molecular mechanisms involved in their host specificity and for detection of E. tarda in aquaculture environments.

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Section: Rastmentioning

confidence: 70%

“…Genomic DNA was extracted from 100 ll of purified phage particles (10 11 PFU/ml) using a standard phenol-chloroform extraction method. A comparison of the genomes of GF-2, MSW-3 and E. ictaluri myovirus phage PEi21 [7,24] was carried out by BLAST analysis. Titanium sequencing platform.…”

Section: Rastmentioning

confidence: 99%

Full-genome sequence of a novel myovirus, GF-2, infecting Edwardsiella tarda: comparison with other Edwardsiella myoviral genomes

et al. 2015

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“…show 99.35% to 99.81% similarities to each other [7]. However, they are differing in chromosomal gene sequence, guanine and cytocine (G+C) content of the chromosome and extrachromosomal element [18][19][20][21][22]. Unlike E. ictaluri the other Edwardsiella spp.…”

Section: Phenotypic and Genotypic Characteristics Of The Bacteriummentioning

confidence: 99%

“…Unlike E. ictaluri the other Edwardsiella spp. chromosome have longer base pair (bp), that is, E. ictaruri has 43,378 bp [20], E. tarda (3,857,0403 bp) [19], E. hoshinae (3,811,650 bp) [22], E. piscicida (3,934,167 bp) [21]. All Edwardsiella spp.…”

Section: Phenotypic and Genotypic Characteristics Of The Bacteriummentioning

confidence: 99%

Edwardsiella Species Infection in Fish Population and Its Status in Ethiopia

Kerie¹,

Nuru²,

Abayneh³

2019

Fish Aquac J

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The genus Edwardsiella (E.) is a Gram-negative, glucose fermentative, catalase positive, and capable of producing H 2 S and indole (except E. ictaluri). It comprises the three previously isolated E. tarda, E. hoshinae, E. ictaluri and the two recently isolated E. piscicida, E. anguillarum species. Except E. hoshinae, other Edwardsiella spp. are pathogenic to fish, and only E. tarda is known to cause human infection. Pathogenic Edwardsiella spp. uses the virulence factors that include flagellin, adhesin, hemolysin, type III and type VI secretion systems to gain entry into and survive within the host. The status and distribution of edwardsiellosis in fish and its public health significance in Ethiopia is poorly understood, and thus needs further studies as aquaculture is growing in the country. Since differentiation of Edwardsiella spp. based only their phenotypic characteristics is less discriminatory than their genotypic characteristics, thus phenotypic studies should always be followed by genetic analysis of the bacterium. Therefore, the objective of this seminar paper is to review Edwardsiella infections in fish, its zoonotic importance and status in Ethiopia.

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“…The abdomen of the fish may become swollen and their eyes may protrude to form a popeyed appearance. Efforts have been made to identify and characterize lytic phages against E. ictaluri 35,36 and to sequence them 35,37 ; however, there is little to no information on the effectiveness of these phages to reduce illnesses or mortalities in cultured catfish or other species.…”

Section: Edwardsiella Ictalurimentioning

confidence: 99%

Bacteriophage remediation of bacterial pathogens in aquaculture: a review of the technology

Richards

2014

Bacteriophage

110

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Complete Genome Sequence of the Edwardsiella ictaluri-Specific Bacteriophage PEi21, Isolated from River Water in Japan

Cited by 12 publications

References 17 publications

Full-genome sequence of a novel myovirus, GF-2, infecting Edwardsiella tarda: comparison with other Edwardsiella myoviral genomes

Full-genome sequence of a novel myovirus, GF-2, infecting Edwardsiella tarda: comparison with other Edwardsiella myoviral genomes

Edwardsiella Species Infection in Fish Population and Its Status in Ethiopia

Bacteriophage remediation of bacterial pathogens in aquaculture: a review of the technology

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