Complete genome sequence of the first non-Asian isolate of Bombyx mori nucleopolyhedrovirus

Abstract

Brazil is one of the largest silk producers in the world. The domesticated silkworm (Bombyx mori) was formally introduced into the country in the twentieth century, and the state of Paraná is the main national producer. During larval stages, B. mori can be afflicted by many different infectious diseases, which lead to substantial losses in silk production. In this work, we describe the structure and complete genome sequence of the first non-Asian isolate of Bombyx mori nucleopolyhedrovirus (BmNPV), the most important silkworm pathogen. The BmNPV-Brazilian isolate is a nucleopolyhedrovirus with singly enveloped nucleocapsids within polyhedral occlusion bodies. Its genome has 126,861 bp with a G + C content of 40.4 %. Phylogenetic analysis clustered the virus with the Japanese strain (BmNPV-T3). As expected, we have detected intra-population variability in the virus sample. Variation along homologous regions (HRs) and bro genes was observed; there were seven HRs, deletion of bro-e, and division of bro-a into two ORFs. The study of baculoviruses allows for a better understanding of virus evolution providing insight for biological control of insect pests or protection against the pernicious disease caused by these viruses.

Characterization of Helicoverpa Zea Single Nucleopolyhedrovirus Isolated in Brazil During the First Old World Bollworm (Noctuidae: Helicoverpa Armigera) Nationwide Outbreak

“…1B). The occlusion bodies purification, polyhedra EM and DNA extraction were performed according to published protocols (Ardisson-Araujo et al 2014). The viral DNA (1-2 µg) was individually cleaved with the restriction enzymes XhoI, BglII, PstI, or BamHI (Promega) according to manufacturer's instructions.…”

Isolation and identification of a new Bombyx mori nucleopolyhedrovirus strain isolated from Yunnan, China

“…The duplication and loss of bro genes among genotypic variants of baculovirus commonly occur 11,12 . As with these baculoviruses, BmNPV exhibits a divergence in bro genes, which is also one of the major differences among ORFs of www.scienceasia.org BmNPV strains [13][14][15] . The function of these genes during baculovirus infection remains unclear, although the properties of some individual bro proteins have been characterized [16][17][18][19] .…”

Genômica, evolução e caracterização funcional de genes de baculovírus

“…However, our results have presented the first clues for explaining nucleotide metabolism gene fixation in baculovirus genomes. Overall, in this context, the identification and sequencing of novel virus species or isolates, especially from countries with high diversity of flora and fauna such as Brazil, has provided a wider empirical database to help understand baculovirus evolution Ardisson-Araujo et al, 2014b;Ardisson-Araujo et al, 2015b;Craveiro et al, 2015;Oliveira et al, 2006).…”

“…Large-scale DNA sequencing provides information on complete viral genomes allowing for "omic" approaches that will eventually facilitate the development of application strategies. Since Brazil is a very diverse country, several baculoviruses have been found and their genomes sequenced Ardisson-Araujo et al, 2014b;Craveiro et al, 2015;Oliveira et al, 2006;Wolff et al, 2008). With this prospect in mind, we have sequenced and analyzed the genome of Diatraea saccharalis granulovirus (DisaGV), the first betabaculovirus isolated from a member of the family Crambidae.…”

“…One hundred µl of the OB-containing suspension (10 9 OBs/ml of ddH2O) were used for SEM according to previously published protocol (Ardisson-Araujo et al, 2014b). For endonuclease restriction analyses, OBs were dissolved in alkaline solution and used to extract DNA .…”