Complete Genome Sequence of the Sugar Cane Endophyte Pseudomonas aurantiaca PB-St2, a Disease-Suppressive Bacterium with Antifungal Activity toward the Plant Pathogen
            <i>Colletotrichum falcatum</i>

Mehnaz, Samina; Bauer, Judith S.; Gross, Harald

doi:10.1128/genomea.01108-13

Cited by 17 publications

(16 citation statements)

References 28 publications

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“…Bacterial culture-containing plates were incubated at 28 o C for 24 h. CAS medium [27] was overlaid on these cultures, and a change in color from blue to orange (hydroxamate-type siderophore) or purple (catechol-type siderophore) was considered as a positive result. Pseudomonas aurantiaca PB-St2 was used as a positive control [17]. Sterilized LB and water were used as the negative control.…”

Section: Detection Of Hcn and Siderophore Production By Using Bioassaysmentioning

confidence: 99%

“…The objectives of this study included selection of fluorescent pseudomonads based on antifungal activity against local pathogens; characterization of the selected Pseudomonas isolates; search for those secondary metabolites that have been previously reported for fluorescent pseudomonads [15]; screen for HCN, siderophore production, and phosphate and zinc solubilization by using bioassays; and observation of the effect of these strains on wheat plant growth by inoculation. P. chlororaphis subspecies aurantiaca strain PB-St2 was previously isolated from sugarcane by our laboratory and its secondary metabolites were reported [16][17][18]. In this study, it was used as a reference/standard for phenazines, the cyclic lipopeptide white line-inducing principle (WLIP) and lahorenoic acid derivatives.…”

Section: Introductionmentioning

confidence: 99%

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Secondary Metabolites Production and Plant Growth Promotion by Pseudomonas chlororaphis and P. aurantiaca Strains Isolated from Cactus, Cotton, and Para Grass

Shahid

Rizwan

Baig

et al. 2017

Journal of Microbiology and Biotechnology

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Fluorescent pseudomonads have been isolated from halophytes, mesophytes, and xerophytes of Pakistan. Among these, eight isolates, GS-1, GS-3, GS-4, GS-6, GS-7, FS-2 (cactus), ARS-38 (cotton), and RP-4 (para grass), showed antifungal activity and were selected for detailed study. Based on biochemical tests and 16S rRNA gene sequences, these were identified as strains of subsp. and . Secondary metabolites of these strains were analyzed by LC-MS. Phenazine-1-carboxylic acid (PCA), 2-hydroxy-phenazine, Cyclic Lipopeptide (white line-inducing principle (WLIP)), and lahorenoic acid A were detected in variable amounts in these strains. PB-St2 was used as a reference as it is known for the production of these compounds. The and genes were amplified to assure that production of these compounds is not an artifact. Indole acetic acid production was confirmed and quantified by HPLC. HCN and siderophore production by all strains was observed by plate assays. These strains did not solubilize phosphate, but five strains were positive for zinc solubilization. Wheat seedlings were inoculated with these strains to observe their effect on plant growth. strains PB-St2 and GS-6 and RP-4 significantly increased both root and shoot dry weights, as compared with uninoculated plants. However, strains FS-2 and ARS-38 significantly increased root and shoot dry weights, respectively. All strains except PB-St2 and ARS-38 significantly increased the root length. This is the first report of the isolation of from cotton and cactus, from para grass, WLIP and lahorenoic acid A production by, and zinc solubilization by and.

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Section: Detection Of Hcn and Siderophore Production By Using Bioassaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Secondary Metabolites Production and Plant Growth Promotion by Pseudomonas chlororaphis and P. aurantiaca Strains Isolated from Cactus, Cotton, and Para Grass

Shahid

Rizwan

Baig

et al. 2017

Journal of Microbiology and Biotechnology

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“…aurantiaca PB-St2, the whole genome sequence [13] was analyzed using antiSMASH 3.0 software, which readily identified three putative QS systems. To obtain more information about these systems, a BLAST search for already known and characterized systems was performed.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, strain PB-St2 was shown to produce three aromatic acids called lahorenoic acids A-C, 2-hydroxyphenazine-1-carboxylic acid, 2,8-dihydroxyphenazine, the lipopeptide WLIP, hydrogen cyanide, and C6-homoserine lactone (HSL) [11, 12]. Genome sequencing of PB-St2 revealed that the strain possesses in addition the biosynthetic capacity to produce the antifungal metabolite pyrrolnitrin, and siderophores of the achromobactin and pyoverdin-type [13]. …”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, AHL-based QS systems are known to be of great importance for many plant-associated biocontrol strains, because QS systems control the production of antibiotics [37–40]. Since the genome sequencing analysis revealed that strain PB-St2 possesses at least three QS systems [13], we began to explore the possibility that PB-St2 produces a variety of AHLs and that AHLs may be involved in regulating the biocontrol properties of PB-St2. Therefore, we sought to (i) identify and chemically characterize all AHLs produced by PB-St2, and (ii) clone and heterologously overexpress genes involved in AHL biosynthesis to prove their function.…”

Section: Introductionmentioning

confidence: 99%

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The Systematic Investigation of the Quorum Sensing System of the Biocontrol Strain Pseudomonas chlororaphis subsp. aurantiaca PB-St2 Unveils aurI to Be a Biosynthetic Origin for 3-Oxo-Homoserine Lactones

et al. 2016

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The shoot endophytic biocontrol strain Pseudomonas chlororaphis subsp. aurantiaca PB-St2 produces a wide range of exoproducts, including enzymes and antibiotics. The production of exoproducts is commonly tightly regulated. In order to get a deeper insight into the regulatory network of PB-St2, the strain was systematically investigated regarding its quorum sensing systems, both on the genetic and metabolic level. The genome analysis of PB-St2 revealed the presence of four putative acyl homoserine lactone (AHL) biosynthesis genes: phzI, csaI, aurI, and hdtS. LC-MS/MS analyses of the crude supernatant extracts demonstrated that PB-St2 produces eight AHLs. In addition, the concentration of all AHL derivatives was quantified time-resolved in parallel over a period of 42 h during the growth of P. aurantiaca PB-St2, resulting in production curves, which showed differences regarding the maximum levels of the AHLs (14.6 nM– 1.75 μM) and the production period. Cloning and heterologous overexpression of all identified AHL synthase genes in Escherichia coli proved the functionality of the resulting synthases PhzI, CsaI, and AurI. A clear AHL production pattern was assigned to each of these three AHL synthases, while the HdtS synthase did not lead to any AHL production. Furthermore, the heterologous expression study demonstrated unequivocally and for the first time that AurI directs the synthesis of two 3-oxo-AHLs.

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Bacterial endophytes: Molecular interactions with their hosts

et al. 2021

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Plant growth promotion has been found associated with plants on the surface (epiphytic), inside (endophytic), or close to the plant roots (rhizospheric). Endophytic bacteria mainly have been researched for their beneficial activities in terms of nutrient availability, plant growth hormones, and control of soil‐borne and systemic pathogens. Molecular communications leading to these interactions between plants and endophytic bacteria are now being unrevealed using multidisciplinary approaches with advanced techniques such as metagenomics, metaproteomics, metatranscriptomics, metaproteogenomic, microRNAs, microarray, chips as well as the comparison of complete genome sequences. More than 400 genes in both the genomes of host plant and bacterial endophyte are up‐ or downregulated for the establishment of endophytism and plant growth‐promoting activity. The involvement of more than 20 genes for endophytism, about 50 genes for direct plant growth promotion, about 25 genes for biocontrol activity, and about 10 genes for mitigation of different stresses has been identified in various bacterial endophytes. This review summarizes the progress that has been made in recent years by these modern techniques and approaches.

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Complete Genome Sequence of the Sugar Cane Endophyte Pseudomonas aurantiaca PB-St2, a Disease-Suppressive Bacterium with Antifungal Activity toward the Plant Pathogen Colletotrichum falcatum

Cited by 17 publications

References 28 publications

Secondary Metabolites Production and Plant Growth Promotion by Pseudomonas chlororaphis and P. aurantiaca Strains Isolated from Cactus, Cotton, and Para Grass

Secondary Metabolites Production and Plant Growth Promotion by Pseudomonas chlororaphis and P. aurantiaca Strains Isolated from Cactus, Cotton, and Para Grass

The Systematic Investigation of the Quorum Sensing System of the Biocontrol Strain Pseudomonas chlororaphis subsp. aurantiaca PB-St2 Unveils aurI to Be a Biosynthetic Origin for 3-Oxo-Homoserine Lactones

Bacterial endophytes: Molecular interactions with their hosts

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