Complete genome sequence of Streptococcus agalactiae strain SA20-06, a fish pathogen associated to meningoencephalitis outbreaks

Pereira, Ulisses de Pádua; Santos, Anderson; Hassan, Syed Shah; Aburjaile, Flávia Figueira; Soares, Siomar de Castro; Ramos, Rommel Thiago Jucá; Carneiro, Adriana Ribeiro; Guimarães, Luís Carlos; Almeida, Sintia Silva de; Diniz, Carlos; Barbosa, Maria Silvanira; Sá, Pablo Gomes de; Ali, Amjad; Bakhtiar, Syeda Marriam; Dorella, Fernanda Alves; Zerlotini, Adhemar; Araújo, Flávio Marcos Gomes; Leite, Laura Hora Rios; Oliveira, Guilherme; Miyoshi, Anderson; Silva, Artur M. S.; Azevedo, Vasco; Figueiredo, Henrique César Pereira

doi:10.4056/sigs.3687314

Cited by 27 publications

(19 citation statements)

References 37 publications

(33 reference statements)

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“…indicated that only parts of the cyl operon are present in this strain (Delannoy et al ., 2012). Interestingly in another nonhaemolytic GBS strain isolated from tilapia an incomplete cyl operon was found containing cylE , cylA and cylB (Liu et al ., 2013; Pereira et al ., 2013), thus supporting the interpretation that these genes are necessary but not sufficient for GBS haemolysis. In fish pathogenic strains, most hypervirulent isolates are nonhaemolytic, indicating that the β-haemolysin is not an important virulence factor in these hosts (C.M.J.…”

Section: Gbs Haemolysin and Pigmentsupporting

confidence: 70%

“…commun.). Genome analysis of veterinary GBS strains reveals a considerable heterogeneity and shows that mechanisms of acquisition, duplication and reshuffling have permitted GBS to adapt to different environmental niches (Tettelin et al ., 2005; Delannoy et al ., 2012; Liu et al ., 2012; Pereira et al ., 2013; Rosinski-Chupin et al ., 2013; Wang et al ., 2013; Zubair et al ., 2013). …”

Section: Gbs Haemolysin and Pigmentmentioning

confidence: 99%

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Group B streptococcal haemolysin and pigment, a tale of twins

Rosa-Fraile¹,

Dramsi²,

Spellerberg³

2014

FEMS Microbiol Rev

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Group B streptococcus [(GBS or Streptococcus agalactiae)] is a leading cause of neonatal meningitis and septicaemia. Most clinical isolates express simultaneously a β-haemolysin/cytolysin and a red polyenic pigment, two phenotypic traits important for GBS identification in medical microbiology. The genetic determinants encoding the GBS haemolysin and pigment have been elucidated and the molecular structure of the pigment has been determined. The cyl operon involved in haemolysin and pigment production is regulated by the major two-component system CovS/R, which coordinates the expression of multiple virulence factors of GBS. Genetic analyses indicated strongly that the haemolysin activity was due to a cytolytic toxin encoded by cylE. However, the biochemical nature of the GBS haemolysin has remained elusive for almost a century because of its instability during purification procedures. Recently, it has been suggested that the haemolytic and cytolytic activity of GBS is due to the ornithine rhamnopolyenic pigment and not to the CylE protein. Here we review and summarize our current knowledge of the genetics, regulation and biochemistry of these twin GBS phenotypic traits, including their functions as GBS virulence factors.

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Section: Gbs Haemolysin and Pigmentsupporting

confidence: 70%

Section: Gbs Haemolysin and Pigmentmentioning

confidence: 99%

Group B streptococcal haemolysin and pigment, a tale of twins

Rosa-Fraile¹,

Dramsi²,

Spellerberg³

2014

FEMS Microbiol Rev

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show abstract

“…agalactiae is also an important pathogen in animals, especially in cattle, in which it causes bovine mastitis. It may also cause meningoencephalitis and septicemia in fish (15).…”

mentioning

confidence: 99%

DNA Microarray-Based Typing of Streptococcus agalactiae Isolates

Nitschke¹,

Slickers²,

Müller³

et al. 2014

J Clin Microbiol

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c Streptococcus agalactiae frequently colonizes the urogenital tract, and it is a major cause of bacterial septicemia, meningitis, and pneumonia in newborns. For typing purposes, a microarray targeting group B streptococcus (GBS) virulence-associated markers and resistance genes was designed and validated with reference strains, as well as clinical and veterinary isolates. Selected isolates were also subjected to multilocus sequence typing. It was observed that putative typing markers, such as alleles of the alpha-like protein or capsule types, vary independently of each other, and they also vary independently from the affiliation to their multilocus sequence typing (MLST)-defined sequence types. Thus, it is not possible to assign isolates to sequence types based on the identification of a single distinct marker, such as a capsule type or alp allele. This suggests the occurrence of frequent genomic recombination. For array-based typing, a set of 11 markers (bac, alp, pil1 locus, pepS8, fbsB, capsule locus, hylB, abiG-I/-II plus Q8DZ34, pil2 locus, nss plus srr plus rogB2, and rgfC/A/D/B) was defined that provides a framework for splitting the tested 448 S. agalactiae isolates into 76 strains that clustered mainly according to MLST-defined clonal complexes. There was evidence for region-and host-specific differences in the population structure of S. agalactiae, as well as an overrepresentation of strains related to sequence type 17 among the invasive isolates. The arrays and typing scheme described here proved to be a convenient tool for genotyping large numbers of clinical/veterinary isolates and thus might help obtain insight into the epidemiology of S. agalactiae.

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“…The in silico immunogenicity of these proteins was not evaluated, which may explain the low efficiency of the majority of tested targets. Currently, other GBS genomes were deposited in GenBank, including strains isolated from other hosts such as cattle and fish (Richards et al, 2011;Delannoy et al, 2012;Liu et al, 2012;Wang et al, 2012;Pereira et al, 2013). Furthermore, new tools for vaccine target prediction are available that estimate the immunogenic potential of proteins, which may improve the choice (and reduce the number) of vaccine targets to be tested (He et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

In silico prediction of conserved vaccine targets in Streptococcus agalactiae strains isolated from fish, cattle, and human samples

Pereira

Soares²,

Blom³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Streptococcus agalactiae (Lancefield group B; group B streptococci) is a major pathogen that causes meningoencephalitis Effective vaccines against the different serotypes or genotypes of pathogenic strains from the various hosts would be useful. We used an in silico strategy to identify conserved vaccine candidates in 15 genomes of group B streptococci strains isolated from human, bovine, and fish samples. The degree of conservation, subcellular localization, and immunogenic potential of S. agalactiae proteins were investigated. We identified 36 antigenic proteins that were conserved in all 15 genomes. Among these proteins, 5 and 23 were shared only by human or fish strains, respectively. These potential vaccine targets may help develop effective vaccines that will help prevent S. agalactiae infection.

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Complete genome sequence of Streptococcus agalactiae strain SA20-06, a fish pathogen associated to meningoencephalitis outbreaks

Cited by 27 publications

References 37 publications

Group B streptococcal haemolysin and pigment, a tale of twins

Group B streptococcal haemolysin and pigment, a tale of twins

DNA Microarray-Based Typing of Streptococcus agalactiae Isolates

In silico prediction of conserved vaccine targets in Streptococcus agalactiae strains isolated from fish, cattle, and human samples

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