Complete Genome Sequence of Streptococcus thermophilus Strain B59671, Which Naturally Produces the Broad-Spectrum Bacteriocin Thermophilin 110

Renye, John A.; Needleman, David S.; Somkuti, George A.; Steinberg, Dennis H.

doi:10.1128/genomea.01213-17

Cited by 7 publications

(9 citation statements)

References 21 publications

(22 reference statements)

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“…For example, the gadB-gadC genes are flanked by transposases elements in S. thermophilus. In S. thermophilus strains ACA-DC 2, B59671, and TH1435, S-ribosylhomocysteine lyase and ribonuclease Y locate, respectively, upstream and downstream of the gadB-gadC operon [47][48][49]. However, for strains ND03 and APC151, DUF1310 family protein and ABC transporter ATP-binding protein locate downstream of gadB-gadC [50,51].…”

Section: Gad System Of Labmentioning

confidence: 99%

Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review

Cui

Miao

Niyaphorn

et al. 2020

IJMS

264

186

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Gamma-aminobutyric acid (GABA) is widely distributed in nature and considered a potent bioactive compound with numerous and important physiological functions, such as anti-hypertensive and antidepressant activities. There is an ever-growing demand for GABA production in recent years. Lactic acid bacteria (LAB) are one of the most important GABA producers because of their food-grade nature and potential of producing GABA-rich functional foods directly. In this paper, the GABA-producing LAB species, the biosynthesis pathway of GABA by LAB, and the research progress of glutamate decarboxylase (GAD), the key enzyme of GABA biosynthesis, were reviewed. Furthermore, GABA production enhancement strategies are reviewed, from optimization of culture conditions and genetic engineering to physiology-oriented engineering approaches and co-culture methods. The advances in both the molecular mechanisms of GABA biosynthesis and the technologies of synthetic biology and genetic engineering will promote GABA production of LAB to meet people’s demand for GABA. The aim of the review is to provide an insight of microbial engineering for improved production of GABA by LAB in the future.

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Section: Gad System Of Labmentioning

confidence: 99%

Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review

Cui

Miao

Niyaphorn

et al. 2020

IJMS

264

186

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“…For example, the locus of a class II bacteriocin-like peptide (blp) was experimentally studied in strains CNRZ1066, LMG 18311, and LMD-9 and it was concluded that it is only functional in the last strain (Hols et al, 2005). In strain B59671, GI 5 carries genes of the blp gene cluster involved in the production of the bacteriocin thermophilin 110 (Renye et al, 2017). Finally, in GI 6 of strain GABA we found a locus containing several genes coding for leader peptides (including mutacin IV, BlpU, and bovicin 255), but transport or immunity proteins seem to be inactive or absent ( Supplementary Table S12A).…”

Section: Genomic Islandsmentioning

confidence: 99%

Comparative Genomics of Streptococcus thermophilus Support Important Traits Concerning the Evolution, Biology and Technological Properties of the Species

et al. 2019

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Streptococcus thermophilus is a major starter for the dairy industry with great economic importance. In this study we analyzed 23 fully sequenced genomes of S. thermophilus to highlight novel aspects of the evolution, biology and technological properties of this species. Pan/core genome analysis revealed that the species has an important number of conserved genes and that the pan genome is probably going to be closed soon. According to whole genome phylogeny and average nucleotide identity (ANI) analysis, most S. thermophilus strains were grouped in two major clusters (i.e., clusters A and B). More specifically, cluster A includes strains with chromosomes above 1.83 Mbp, while cluster B includes chromosomes below this threshold. This observation suggests that strains belonging to the two clusters may be differentiated by gene gain or gene loss events. Furthermore, certain strains of cluster A could be further subdivided in subgroups, i.e., subgroup I (ASCC 1275, DGCC 7710, KLDS SM, MN-BM-A02, and ND07), II (MN-BM-A01 and MN-ZLW-002), III (LMD-9 and SMQ-301), and IV (APC151 and ND03). In cluster B certain strains formed one distinct subgroup, i.e., subgroup I (CNRZ1066, CS8, EPS, and S9). Clusters and subgroups observed for S. thermophilus indicate the existence of lineages within the species, an observation which was further supported to a variable degree by the distribution and/or the architecture of several genomic traits. These would include exopolysaccharide (EPS) gene clusters, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs)-CRISPR associated (Cas) systems, as well as restriction-modification (R-M) systems and genomic islands (GIs). Of note, the histidine biosynthetic cluster was found present in all cluster A strains (plus strain NCTC12958 T) but was absent from all strains in cluster B. Other loci related to lactose/galactose catabolism and urea metabolism, aminopeptidases, the majority of amino acid and peptide transporters, as well as amino acid biosynthetic pathways

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“…Similar to other S. thermophilus strains (6, 13), the ST109 genome contained 13.5% nonfunctional genes; however, ST106 possessed 323 pseudogenes, corresponding to 16.8% of the predicted coding sequences. Previous studies showed that thermophilin 109 was naturally produced and may be encoded within the blp gene cluster (4, 7).…”

Section: Announcementmentioning

confidence: 74%

“…The blp gene cluster has been characterized for S. thermophilus strains LMD-9 (3), ST106, and ST118 (4), where bacteriocin production was induced with a recombinant or synthetic quorum sensing peptide, BlpC-30, and for strain B59671 (5, 6), where production occurs naturally.…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequences of Bacteriocin-Producing Streptococcus thermophilus Strains ST106 and ST109

Renye

Needleman

Steinberg

2019

Microbiol Resour Announc

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Complete Genome Sequence of Streptococcus thermophilus Strain B59671, Which Naturally Produces the Broad-Spectrum Bacteriocin Thermophilin 110

Cited by 7 publications

References 21 publications

Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review

Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review

Comparative Genomics of Streptococcus thermophilus Support Important Traits Concerning the Evolution, Biology and Technological Properties of the Species

Complete Genome Sequences of Bacteriocin-Producing Streptococcus thermophilus Strains ST106 and ST109

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