Complete Genome Sequence of Stenotrophomonas maltophilia Myophage Moby

Vicary, Alison C.; Newkirk, Heather; Moreland, Russell; Gonzalez, Carlos F.; Liu, Mei; Ramsey, Jolene; Leavitt, Justin C.

doi:10.1128/mra.01422-19

Cited by 7 publications

(5 citation statements)

References 24 publications

(3 reference statements)

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“…It is possible that the competitive advantage of type IV pili to aid in the colonization of plants [ 2 , 31 ] has selected for the use of these structures as phage receptors in soil microbes. It is unknown if the type IV pilus is a favoured receptor of S. maltophilia phages isolated from other environmental sources, such as water and sewage, as the receptors for these phages have not been examined [ 12 , 13 , 14 , 15 , 16 , 22 , 23 , 24 , 32 ]. However, the T4-like virulent phage Smp14 has been observed to bind to the poles of S. maltophilia cells by TEM [ 21 ] where type IV pili are normally expressed.…”

Section: Resultsmentioning

confidence: 99%

“…The use of phages for human therapy requires extensive phage characterization, including host range analysis, genomic characterization and identification of potential moron genes, lifestyle determination and identification of cell surface receptors. In the last two decades, numerous phages against S. maltophilia have been isolated and characterized to various extents for their therapeutic potential [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ]. Herein we describe the isolation and characterization of a novel S. maltophilia phage, AXL3, unrelated to any phages currently sequenced in the NCBI database.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of the Novel Bacteriophage AXL3 against Stenotrophomonas maltophilia

McCutcheon

Lin

Dennis

2020

IJMS

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The rapid increase in the number of worldwide human infections caused by the extremely antibiotic resistant bacterial pathogen Stenotrophomonas maltophilia is cause for concern. An alternative treatment solution in the post-antibiotic era is phage therapy, the use of bacteriophages to selectively kill bacterial pathogens. In this study, the novel bacteriophage AXL3 (vB_SmaS-AXL_3) was isolated from soil and characterized. Host range analysis using a panel of 29 clinical S. maltophilia isolates shows successful infection of five isolates and electron microscopy indicates that AXL3 is a member of the Siphoviridae family. Complete genome sequencing and analysis reveals a 47.5 kb genome predicted to encode 65 proteins. Functionality testing suggests AXL3 is a virulent phage and results show that AXL3 uses the type IV pilus, a virulence factor on the cell surface, as its receptor across its host range. This research identifies a novel virulent phage and characterization suggests that AXL3 is a promising phage therapy candidate, with future research examining modification through genetic engineering to broaden its host range.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of the Novel Bacteriophage AXL3 against Stenotrophomonas maltophilia

McCutcheon

Lin

Dennis

2020

IJMS

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“…Currently isolated S. maltophilia phages include diverse morphological types [27] and range in size from the smallest filamentous phage ΦSHP2 of 5819 bp [84] to the largest SMA5 estimated to be ~250 kbp [28]. With its size of 161,350 bp, phage Ps15 has been placed in the group consisting of six large Myoviridae phages: Mendera [85], Moby [86], IME-SM1, YB07, Marzo and BUCT648 that belong to the new Menderavirus genus. Mendera and Moby have been isolated in America, IME-SM1 and YB07 in China and Ps15 in Australia from the same type of environment: wastewater.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to Stenotrophomonas maltophilia Clinical Ocular Isolates

Damnjanovic

Vázquez-Campos

Elliott³

et al. 2022

Viruses

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Recent acknowledgment that multidrug resistant Stenotrophomonas maltophilia strains can cause severe infections has led to increasing global interest in addressing its pathogenicity. While being primarily associated with hospital-acquired respiratory tract infections, this bacterial species is also relevant to ophthalmology, particularly to contact lens-related diseases. In the current study, the capacity of Stenotrophomonas phage vB_SmaM_Ps15 to infect ocular S. maltophilia strains was investigated to explore its future potential as a phage therapeutic. The phage proved to be lytic to a range of clinical isolates collected in Australia from eye swabs, contact lenses and contact lens cases that had previously shown to be resistant to several antibiotics and multipurpose contact lenses disinfectant solutions. Morphological analysis by transmission electron microscopy placed the phage into the Myoviridae family. Its genome size was 161,350 bp with a G + C content of 54.2%, containing 276 putative protein-encoding genes and 24 tRNAs. A detailed comparative genomic analysis positioned vB_SmaM_Ps15 as a new species of the Menderavirus genus, which currently contains six very similar globally distributed members. It was confirmed as a virulent phage, free of known lysogenic and pathogenicity determinants, which supports its potential use for the treatment of S. maltophilia eye infections.

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“…Four S. maltophilia phages were isolated by a group from the Center for Phage Technology (CPT) at Texas A&M University. Phages Ponderosa [163] and Pokken [164] are Podoviridae phages isolated from water samples and phages Moby [165] and Mendera [166] are Myoviridae phages isolated from wastewater. Complete genomic characterization for each phage is available on NCBI, however, no experimental data regarding host range, lifecycle, or phage infection dynamics were provided to evaluate the suitability of these phages for therapeutic use.…”

Section: S Maltophilia Phagesmentioning

confidence: 99%

The Potential of Phage Therapy against the Emerging Opportunistic Pathogen Stenotrophomonas maltophilia

McCutcheon

Dennis

2021

Viruses

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The isolation and characterization of bacteriophages for the treatment of infections caused by the multidrug resistant pathogen Stenotrophomonas maltophilia is imperative as nosocomial and community-acquired infections are rapidly increasing in prevalence. This increase is largely due to the numerous virulence factors and antimicrobial resistance genes encoded by this bacterium. Research on S. maltophilia phages to date has focused on the isolation and in vitro characterization of novel phages, often including genomic characterization, from the environment or by induction from bacterial strains. This review summarizes the clinical significance, virulence factors, and antimicrobial resistance mechanisms of S. maltophilia, as well as all phages isolated and characterized to date and strategies for their use. We further address the limited in vivo phage therapy studies conducted against this bacterium and discuss the future research needed to spearhead phages as an alternative treatment option against multidrug resistant S. maltophilia.

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Complete Genome Sequence of Stenotrophomonas maltophilia Myophage Moby

Cited by 7 publications

References 24 publications

Isolation and Characterization of the Novel Bacteriophage AXL3 against Stenotrophomonas maltophilia

Isolation and Characterization of the Novel Bacteriophage AXL3 against Stenotrophomonas maltophilia

Characterisation of Bacteriophage vB_SmaM_Ps15 Infective to Stenotrophomonas maltophilia Clinical Ocular Isolates

The Potential of Phage Therapy against the Emerging Opportunistic Pathogen Stenotrophomonas maltophilia

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