Complete Genome Sequence of Stenotrophomonas maltophilia Podophage Ptah

Berg, Amelia; Tate, Nathaniel; Clark, James; Le, Tram; Burrowes, Ben; Liu, Mei

doi:10.1128/mra.00137-22

Cited by 1 publication

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The structure of the receptor-binding proteins of StenM_174 was predicted using AlphaFold2. A comparative analysis of the genome showed that the Stenotrophomonas phage StenM_174, along with the phages Ponderosa [ 24 ], Pepon [ 25 ], Ptah [ 26 ], and TS-10 (GenBank ID OK018136), can be members of the new putative genus Ponderosavirus within the Autographiviridae family. In addition, the analyzed data suggest a new subfamily within this family.…”

Section: Introductionmentioning

confidence: 99%

StenM_174: A Novel Podophage That Infects a Wide Range of Stenotrophomonas spp. and Suggests a New Subfamily in the Family Autographiviridae

Morozova,

Yakubovskij,

Baykov

et al. 2023

Viruses

View full text Add to dashboard Cite

Stenotrophomonas maltophilia was discovered as a soil bacterium associated with the rhizosphere. Later, S. maltophilia was found to be a multidrug-resistant hospital-associated pathogen. Lytic bacteriophages are prospective antimicrobials; therefore, there is a need for the isolation and characterization of new Stenotrophomonas phages. The phage StenM_174 was isolated from litter at a poultry farm using a clinical strain of S. maltophilia as the host. StenM_174 reproduced in a wide range of clinical and environmental strains of Stenotrophomonas, mainly S. maltophilia, and it had a podovirus morphotype. The length of the genomic sequence of StenM_174 was 42,956 bp, and it contained 52 putative genes. All genes were unidirectional, and 31 of them encoded proteins with predicted functions, while the remaining 21 were identified as hypothetical ones. Two tail spike proteins of StenM_174 were predicted using AlphaFold2 structural modeling. A comparative analysis of the genome shows that the Stenotrophomonas phage StenM_174, along with the phages Ponderosa, Pepon, Ptah, and TS-10, can be members of the new putative genus Ponderosavirus in the Autographiviridae family. In addition, the analyzed data suggest a new subfamily within this family.

show abstract

Section: Introductionmentioning

confidence: 99%