Complete Genome Sequence of Staphylococcus aureus Siphophage Sebago

Abstract

Here, we introduce the genome of Sebago, a 43,878-bp siphophage that infects Staphylococcus aureus. Sebago carries 70 proteins and is most closely related to StauST398, a Phietavirus.

Horizontal transfer and phylogenetic distribution of the immune evasion factor tarP

“…Accordingly, IEC was significantly enriched in S. aureus genomes of human origin (p < 10 −15 , Fisher's exact test) (Supplementary Figure 1). tarP was usually located on ΦSa3int phages in S. aureus clones harboring the IEC element, whereas it was present on many different phages in S. aureus clones lacking the IEC element, including ΦSa1int, ΦSa2int, ΦSa5int, ΦSa7int, and the newly described ΦSebago (Klotz et al, 2019) that was previously misidentified as Sa9int (Figure 1D) (Gerlach et al, 2018). In addition, we observed that tarP is 40% more frequent in strains lacking the IEC element than in those with IEC (p < 10 −13 by Fisher's exact test), suggesting that tarP is more prevalent in S. aureus clones from non-human animals (Figure 1E).…”

“…aureus clones harboring the IEC element, whereas it was present on many different phages in S . aureus clones lacking the IEC element, including ΦSa1int, ΦSa2int, ΦSa5int, ΦSa7int, and the newly described ΦSebago ( Klotz et al, 2019 ) that was previously misidentified as Sa9int ( Figure 1D ) ( Gerlach et al, 2018 ). In addition, we observed that tarP is 40% more frequent in strains lacking the IEC element than in those with IEC ( p < 10 −13 by Fisher’s exact test), suggesting that tarP is more prevalent in S .…”