Complete Genome Sequence of Serratia marcescens Siphophage Serbin

Williams, Eric A.; Hopson, Helena; Rodriguez, A. Y.; Kongari, Rohit; Bonasera, Rachele; Hernandez-Morales, Adriana; Liu, Mei

doi:10.1128/mra.00422-19

Cited by 4 publications

(5 citation statements)

References 18 publications

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“…Additionally, PhiOS31_p18 and _p27 predicted the DEAD-like helicase and lysozyme, respectively, have homologous counterparts in Eta. Four putative tail structural proteins of OS31 (PhiOS31_p52-53 and _p55-56) share similarities with the siphovirus Scapp [ 81 ] (sequence identity between 38–43%) and one (PhiOS31_p57, 33% sequence identity) with the siphovirus Serbin [ 82 ] ( Table S4 , Figure 3 b). The last one is a putative tail fiber protein.…”

Section: Resultsmentioning

confidence: 99%

Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine

Bujak

Decewicz

Kamiński

et al. 2020

IJMS

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Bacteria of the genus Serratia inhabit a variety of ecological niches like water, soil, and the bodies of animals, and have a wide range of lifestyles. Currently, the complete genome sequences of 25 Serratia phages are available in the NCBI database. All of them were isolated from nutrient-rich environments like sewage, with the use of clinical Serratia strains as hosts. In this study, we identified a novel Serratia myovirus named vB_SspM_BZS1. Both the phage and its host Serratia sp. OS31 were isolated from the same oligotrophic environment, namely, an abandoned gold mine (Zloty Stok, Poland). The BZS1 phage was thoroughly characterized here in terms of its genomics, morphology, and infection kinetics. We also demonstrated that Serratia sp. OS31 was lysogenized by mitomycin-inducible siphovirus vB_SspS_OS31. Comparative analyses revealed that vB_SspM_BZS1 and vB_SspS_OS31 were remote from the known Serratia phages. Moreover, vB_SspM_BZS1 was only distantly related to other viruses. However, we discovered similar prophage sequences in genomes of various bacteria here. Additionally, a protein-based similarity network showed a high diversity of Serratia phages in general, as they were scattered across nineteen different clusters. In summary, this work broadened our knowledge on the diverse relationships of Serratia phages.

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Section: Resultsmentioning

confidence: 99%

Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine

Bujak

Decewicz

Kamiński

et al. 2020

IJMS

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“…However, as of May 2020, this number has increased to more than 20 phage genomes, where mostly S. marcescens is indicated as host. This comprises the six Myoviridae phages, “MyoSmar” [ 30 ], “Moabite” [ 31 ], “MTx” [ 32 ], “Muldoon” [ 33 ], PS2 [ 17 ], vB_SmaM_2050HW [ 18 ], and “BF” [ 29 ], the four Siphoviridae phages “Slocum” [ 34 ], “Serbin” [ 35 ], “Scapp” [ 36 ], and “Eta” [ 28 ], as well as the three Podoviridae -phages “Pila” [ 37 ], “Parlo” [ 38 ], and “SM9-3Y” [ 24 ]. Furthermore, the genome database contains the S. marcescens phage vB_SmaM_2050HW1 which belongs to the new family Ackermannviridae [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

Tackling Intrinsic Antibiotic Resistance in Serratia marcescens with a Combination of Ampicillin/Sulbactam and Phage SALSA

et al. 2020

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During the antibiotic crisis, bacteriophages (briefly phages) are increasingly considered as potential antimicrobial pillars for the treatment of infectious diseases. Apart from acquired drug resistance, treatment options are additionally hampered by intrinsic, chromosomal-encoded resistance. For instance, the chromosomal ampC gene encoding for the AmpC-type β-lactamases is typically present in a number of nosocomial pathogens, including S. marcescens. In this study, phage SALSA (vB_SmaP-SALSA), with lytic activity against clinical isolates of S. marcescens, was isolated from effluent. Besides phage characterization, the aim of this study was to evaluate whether a synergistic effect between the antibiotic ampicillin/sulbactam (SAM) and phage can be achieved despite intrinsic drug resistance. Phage SALSA belongs to the Podoviridae family and genome-wide treeing analysis groups this phage within the phylogenetic radiation of T7-like viruses. The genome of Phage SALSA consists of 39,933 bp, which encode for 49 open reading frames. Phage SALSA was able to productively lyse 5 out of 20 clinical isolates (25%). A bacterial challenge with phage alone in liquid medium revealed that an initial strong bacterial decline was followed by bacterial re-growth, indicating the emergence of phage resistance. In contrast, the combination of SAM and phage, together at various concentrations, caused a complete bacterial eradication, confirmed by absorbance measurements and the absence of colony forming units after plating. The data show that it is principally possible to tackle the axiomatic condition of intrinsic drug resistance with a dual antimicrobial approach, which could be extended to other clinically relevant bacteria.

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“…37,38 S. marcescens is one of the pathogenic bacteria and many strains of this bacterium have been sequenced at genome and proteome levels. 10,18,20,22,[39][40][41] To the best of our knowledge, the genome of S. marcescens (ATCC 13880) sequence is available and the proteome of the same is not available till date. 10 Hence, this study was performed to understand the proteome of S. marcescens (ATCC 13880).…”

Section: Discussionmentioning

confidence: 99%

Proteomic profiling of Serratia marcescens by high-resolution mass spectrometry

2020

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Introduction: Serratia marcescens, an opportunistic human pathogen, is reported as an important cause of nosocomial infection and outbreaks. Although the genome of S. marcescens (ATCC 13880) was completely sequenced by 2014, there are no studies on the proteomic profile of the organism. The objective of the present study is to analyze the protein profile of S. marcescens (ATCC 13880) using a high resolution mass spectrometry (MS). Methods: Serratia marcescens ATCC 13880 strain was grown in Luria-Bertani broth and the protein extracted was subjected to trypsin digestion, followed by basic reverse phase liquid chromatography fractionation. The peptide fractions were then analysed using Orbitrap Fusion Mass Spectrometry and the raw MS data were processed in Proteome Discoverer software. Results: The proteomic analysis identified 15 009 unique peptides mapping to 2541 unique protein groups, which corresponds to approximately 54% of the computationally predicted protein-coding genes. Bioinformatic analysis of these identified proteins showed their involvement in biological processes such as cell wall organization, chaperone-mediated protein folding and ATP binding. Pathway analysis revealed that some of these proteins are associated with bacterial chemotaxis and beta-lactam resistance pathway. Conclusion: To the best of our knowledge, this is the first high-throughput proteomics study of S. marcescens (ATCC 13880). These novel observations provide a crucial baseline molecular profile of the S. marcescens proteome which will prove to be helpful for the future research in understanding the host-pathogen interactions during infection, elucidating the mechanism of multidrug resistance, and developing novel diagnostic markers or vaccine for the disease.

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Complete Genome Sequence of Serratia marcescens Siphophage Serbin

Abstract: Serratia marcescens is an opportunistic human pathogen that is known to cause hospital-acquired respiratory and urinary tract infections. Here, we announce the complete genome sequence and the features of S. marcescens phage Serbin.

Cited by 4 publications

References 18 publications

Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine

Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine

Tackling Intrinsic Antibiotic Resistance in Serratia marcescens with a Combination of Ampicillin/Sulbactam and Phage SALSA

Proteomic profiling of Serratia marcescens by high-resolution mass spectrometry

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