Complete Genome Sequence of Salmonella enterica Serovar Typhimurium Myophage Mushroom

Tolen, Tamra N.; Xie, Yicheng; Hernández, Adriana C.; Everett, Gabriel F. Kuty

doi:10.1128/genomea.00154-15

Cited by 9 publications

(11 citation statements)

References 17 publications

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“…A comparison using the BLASTn algorithm of these phage genomes showed that the WHR phage’s top BLAST hit is a phage previously sequenced, named Salmonella virus Mushroom, with GenBank acc: KP143762.1 [62], and the NHR phage’s first BLAST result is Salmonella phage FelixO1, with GenBank acc: AF320576.1 [61]. A BLASTn comparison between WHR and NHR phages showed 97% nucleotide identity, shared among their genomes.…”

Section: Resultsmentioning

confidence: 99%

Two Phages of the Genera Felixunavirus Subjected to 12 Hour Challenge on Salmonella Infantis Showed Distinct Genotypic and Phenotypic Changes

Rivera

Hudson

Denes

et al. 2019

Viruses

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Salmonella Infantis is considered in recent years an emerging Salmonella serovar, as it has been associated with several outbreaks and multidrug resistance phenotypes. Phages appear as a possible alternative strategy to control Salmonella Infantis (SI). The aims of this work were to characterize two phages of the Felixounavirus genus, isolated using the same strain of SI, and to expose them to interact in challenge assays to identify genetic and phenotypic changes generated from these interactions. These two phages have a shared nucleotide identity of 97% and are differentiated by their host range: one phage has a wide host range (lysing 14 serovars), and the other has a narrow host range (lysing 6 serovars). During the 12 h challenge we compared: (1) optical density of SI, (2) proportion of SI survivors from phage-infected cultures, and (3) phage titer. Isolates obtained through the assays were evaluated by efficiency of plating (EOP) and by host-range characterization. Genomic modifications were characterized by evaluation of single nucleotide polymorphisms (SNPs). The optical density (600 nm) of phage-infected SI decreased, as compared to the uninfected control, by an average of 0.7 for SI infected with the wide-host-range (WHR) phage and by 0.3 for SI infected with the narrow-host-range (NHR) phage. WHR phage reached higher phage titer (7 × 1011 PFU/mL), and a lower proportion of SI survivor was obtained from the challenge assay. In SI that interacted with phages, we identified SNPs in two genes (rfaK and rfaB), which are both involved in lipopolysaccharide (LPS) polymerization. Therefore, mutations that could impact potential phage receptors on the host surface were selected by lytic phage exposure. This work demonstrates that the interaction of Salmonella phages (WHR and NHR) with SI for 12 h in vitro leads to emergence of new phenotypic and genotypic traits in both phage and host. This information is crucial for the rational design of phage-based control strategies.

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Section: Resultsmentioning

confidence: 99%

Two Phages of the Genera Felixunavirus Subjected to 12 Hour Challenge on Salmonella Infantis Showed Distinct Genotypic and Phenotypic Changes

Rivera

Hudson

Denes

et al. 2019

Viruses

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“…The BLASTn results showed that the closest neighbors of phages VSe11 and VSe102 are the Salmonella phages Mushroom (GenBank accession no. KP143762) ( 13 ), SPT-1 (JX181822), and Si3 (KY626162). All of them encode core proteins inherent to Felix O1-like phages of the family Myoviridae subfamily Ounavirinae ( 14 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequences of Two Salmonella Viruses, VSe11 and VSe102 (Family Myoviridae , Subfamily Ounavirinae ), with a Very High Degree of Similarity

et al. 2018

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“…They are classified as members of the subfamily Ounavirinae [12]. The FO1-like Salmonella phages Mushroom and FO1a are components of commercially available phage cocktails (IntestiPhage™ and PhageGuard S™, respectively) [13, 14]. GJ1-like phages are also virulent myoviruses.…”

Section: Introductionmentioning

confidence: 99%

A major-capsid-protein-based multiplex PCR assay for rapid identification of selected virulent bacteriophage types

et al. 2019

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Bacteriophages represent a promising alternative for controlling pathogenic bacteria. They are ubiquitous in the environment, and their isolation is usually simple and fast. However, not every phage is suitable for biocontrol applications. It must be virulent (i.e., strictly lytic), non-transducing, and safe. We have developed a method for identifying selected types of virulent phages at an early stage of the isolation process to simplify the search for suitable candidates. Using the major capsid protein (MCP) as a phylogenetic marker, we designed degenerate primers for the identification of Felix O1-, GJ1-, N4-, SP6-, T4-, T7-, and Vi1-like phages in multiplex PCR setups with single phage plaques as templates. Performance of the MCP PCR assay was evaluated with a set of 26 well-characterized phages. Neither false-positive nor false-negative results were obtained. In addition, 154 phages from enrichment cultures from various environmental samples were subjected to MCP PCR analysis. Eight of them, specific for Salmonella enterica , Escherichia coli , or Erwinia amylovora , belonged to one of the selected phage types. Their PCR-based identification was successfully confirmed by pulsed-field gel electrophoresis of the phage genomes, electron microscopy, and sequencing of the amplified mcp gene fragment. The MCP PCR assay was shown to be a simple method for preliminary assignment of new phages to a certain group and thus to identify candidates for biocontrol immediately after their isolation. Given that sufficient sequence data are available, this method can be extended to any phage group of interest. Electronic supplementary material The online version of this article (10.1007/s00705-019-04148-6) contains supplementary material, which is available to authorized users.

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Complete Genome Sequence of Salmonella enterica Serovar Typhimurium Myophage Mushroom

Cited by 9 publications

References 17 publications

Two Phages of the Genera Felixunavirus Subjected to 12 Hour Challenge on Salmonella Infantis Showed Distinct Genotypic and Phenotypic Changes

Two Phages of the Genera Felixunavirus Subjected to 12 Hour Challenge on Salmonella Infantis Showed Distinct Genotypic and Phenotypic Changes

Complete Genome Sequences of Two Salmonella Viruses, VSe11 and VSe102 (Family Myoviridae , Subfamily Ounavirinae ), with a Very High Degree of Similarity

A major-capsid-protein-based multiplex PCR assay for rapid identification of selected virulent bacteriophage types

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