Complete Genome Sequence of Pseudomonas aeruginosa XN-1, Isolated from the Sputum of a Severe Pneumonia Patient

Gao, Chen; Wang, Ying; Zhang, Yi; Wei, Jinning; Cheng, Xin; Zhang, Jin; Zou, Quanming; Gu, Jiang

doi:10.1128/mra.00653-20

Cited by 5 publications

(3 citation statements)

References 21 publications

(26 reference statements)

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“…Clinical strains of A. baumannii SJZ24, SJZ26, and SJZ09 were isolated from Bethune International Peace Hospital in Shijiazhuang, China, in 2016 ( Zeng et al, 2019 ). P. aeruginosa strain XN-1 ( Gao et al, 2020 ), K. pneumoniae strain YBQ ( Liu et al, 2020 ), and S. pneumoniae were isolated from Chongqing Southwest Hospital. S. aureus strain KM-22 was isolated from the Second Affiliated Hospital of Kunming Medical University ( Zeng et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Vaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice

Zeng

et al. 2021

eLife

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Vaccination strategies for rapid protection against multidrug-resistant bacterial infection are very important, especially for hospitalized patients who have high risk of exposure to these bacteria. However, few such vaccination strategies exist due to a shortage of knowledge supporting their rapid effect. Here, we demonstrated that a single intranasal immunization of inactivated whole cell of Acinetobacter baumannii elicits rapid protection against broad A. baumannii-infected pneumonia via training of innate immune response in Rag1-/- mice. Immunization-trained alveolar macrophages (AMs) showed enhanced TNF-α production upon restimulation. Adoptive transfer of immunization-trained AMs into naive mice mediated rapid protection against infection. Elevated TLR4 expression on vaccination-trained AMs contributed to rapid protection. Moreover, immunization-induced rapid protection was also seen in Pseudomonas aeruginosa and Klebsiella pneumoniae pneumonia models, but not in Staphylococcus aureus and Streptococcus pneumoniae model. Our data reveal that a single intranasal immunization induces rapid and efficient protection against certain Gram-negative bacterial pneumonia via training AMs response, which highlights the importance and the possibility of harnessing trained immunity of AMs to design rapid-effecting vaccine.

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Section: Methodsmentioning

confidence: 99%

Vaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice

Zeng

et al. 2021

eLife

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“…The minimum inhibitory concentrations (MICs) of peptides against bacteria including A. baumannii (ATCC 19606), multidrug-resistant A. baumannii (MDRAB), 46 S. aureus (ATCC 25923), methicillin-resistant S. aureus (MRSA, ATCC 43300), E. coli (ATCC 25922), multidrug-resistant E. coli (MDREC), 47 P. aeruginosa (PAO1), and multidrug-resistant P. aeruginosa (MDRPA), 48 were determined using the broth microdilution method recommended by the Clinical and Laboratory Standards Institute (CLSI) with modifications reported by the Hancock laboratory. 49 P001, P002, and P076 peptides were prepared in 0.01% acetic acid containing 0.2% BSA at concentrations of 6.25, 12.5, 25, 50, 100, 200, 400, 800, 1600, and 3200 μg/mL.…”

Section: Methodsmentioning

confidence: 99%

Exploring the repository ofde novodesigned bifunctional antimicrobial peptides through deep learning

Dong,

Liu,

Liu

et al. 2024

Preprint

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Antimicrobial peptides (AMPs) are attractive candidates to combat antibiotic resistance for their capability to target bio-membranes and restrict a wide range of pathogens. It is a daunting challenge to discover novel AMPs due to their sparse distributions in a vast peptide universe, especially for peptides that demonstrate potencies for both bacterial membranes and viral envelopes. Here we establish ade novoAMP design framework by bridging a deep generative module and a graph-encoding activity regressor. The generative module learns hidden ‘grammars’ of AMP features and produces candidates sequentially pass antimicrobial predictor and antiviral classifiers. We discover three bifunctional AMPs and experimentally validated their abilities to inhibit a spectrum of pathogensin vitroand in animal models. Notably, P076 is a highly potent bactericide with the minimal inhibitory concentration of 0.21 μM against multidrug-resistantA. baumannii, while P002 broadly inhibits five enveloped viruses. Our study provides feasible means to uncover sequences that simultaneously encode antimicrobial and antiviral activities, thus bolstering the function spectra of AMPs to combat a wide range of drug-resistant infections.

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“…Six- to eight-week-old specific pathogen-free female BALB/c mice were purchased from Beijing Vital River Laboratory Animal Technology Company Limited (Beijing, China). PA XN-1 (CCTCC M2015730) was isolated from the sputum of a patient with severe pneumonia at Southwest Hospital in Chongqing, China [ 18 ]. Sera were collected from PA-infected convalescent patients and healthy donors.…”

Section: Methodsmentioning

confidence: 99%

A novel structurally identified epitope delivered by macrophage membrane-coated PLGA nanoparticles elicits protection against Pseudomonas aeruginosa

et al. 2022

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The increasing prevalence of antibiotic resistance by Pseudomonas aeruginosa (PA) raises an urgent need for an effective vaccine. The outer membrane proteins of PA, especially those that are upregulated during infection, are ideal vaccine targets. However, the strong hydrophobicity of these proteins hinders their application for this purpose. In this study, we selected eight outer membrane proteins from PA with the most significantly upregulated expression. Their extracellular loops were analyzed and screened by using sera from patients who had recovered from PA infection. As a result, a novel immunogenic epitope (Ep167-193) from PilY1 (PA4554) was found. Moreover, we constructed a macrophage membrane-coated PLGA (poly lactic-co-glycolic acid) nanoparticle vaccine carrying PilY1 Ep167-193 (PNPs@M-Ep167-193) that elicits a Th2 immune response and confers adequate protection in mice. Our data furnished the promising vaccine candidate PNPs@M-Ep167-193 while providing additional evidence for structure-based epitope identification and vaccine design. Graphical Abstract

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Complete Genome Sequence of Pseudomonas aeruginosa XN-1, Isolated from the Sputum of a Severe Pneumonia Patient

Cited by 5 publications

References 21 publications

Vaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice

Vaccination induces rapid protection against bacterial pneumonia via training alveolar macrophage in mice

Exploring the repository ofde novodesigned bifunctional antimicrobial peptides through deep learning

A novel structurally identified epitope delivered by macrophage membrane-coated PLGA nanoparticles elicits protection against Pseudomonas aeruginosa

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