Complete genome sequence of Propionibacterium freudenreichii DSM 20271T

Abstract

Propionibacterium freudenreichii subsp. freudenreichii DSM 20271T is the type strain of species Propionibacterium freudenreichii that has a long history of safe use in the production dairy products and B12 vitamin. P. freudenreichii is the type species of the genus Propionibacterium which contains Gram-positive, non-motile and non-sporeforming bacteria with a high G + C content. We describe the genome of P. freudenreichii subsp. freudenreichii DSM 20271T consisting of a 2,649,166 bp chromosome containing 2320 protein-coding genes and 50 RNA-only encoding genes.

Sequencing and Analysis of the Genome of Propionibacterium freudenreichii T82 Strain: Importance for Industry

“…The otsA, otsB, and treS genes were previously identified in NIZO B365, DSM 20271, and CIRM-BIA1 strains. In the P. freudenreichii T82 strain, nucleotide sequences encoding these three genes are similar to those reported by Cardoso et al [29], Koskinen et al [12], and Falentin et al [11], respectively: 98.45% (NIZO B365) and 100% (DSM and CIRM-BIA1); 99.07% (NIZO B365), 99.88% (DSM), and 99.65% (CIRM-BIA1); and 99.78% (NIZO B365), 99.78% (DSM 20271), and 99.89% (CIRM-BIA1).…”

“…Genome analysis showed that there are complete synthesis pathways of almost all these cofactors in the T82 strain ( Figures S1-S3, S5, and S7), except for pantothenate ( Figure S7) and biotin ( Figure S9). Results described by Koskinen et al [12] and Falentin et al [11] also show that PAB have to be supplemented by the addition to the medium of B5 and B7 vitamins. Moreover, according to the obtained results, it is possible that the P. freudenreichii T82 strain can produce folate (B9 from chorismite, which is also produced by the T82 strain; Figure S10) ( Figure S5), thiamine ( Figure S1), and pyridoxal 5 -phosphate (active form of B6) ( Figure S6).…”

“…shermanii CIRM-BIA1 [11] and P. freudenreichii ssp. freudenreichii DSM 20271 [12] strains have been described in the literature. To fully exploit the biotechnological potential of bacteria of the genus Propionibacterium, it is necessary to know their specific molecular properties, which is greatly facilitated by the knowledge of the genome.…”

Secretome profiling of Propionibacterium freudenreichii reveals highly variable responses even among the closely related strains

“…Previous in vivo studies have confirmed that P. freudenreichii can adapt to the porcine colon by altering protein expression and energy substrate utilization (Saraoui et al, 2013). Evolutionary adaptation and probiotic potentials of P. freudenreichii strains have previously been assessed by defining the genome sequences of three dairy-associated strains DSM 20271 T , CIRM BIA 1T and JS (Falentin et al, 2010;Koskinen et al, 2015;Ojala et al, 2017). Compared with the genome of the Acidipropionibacterium strain ATCC4875 (Parizzi et al, 2012), the P. freudenreichii strains have smaller genomes (roughly 3.6 Mbp versus 2.6 Mbp), and although many common genes could be identified, the genomes of the two species vary significantly (Parizzi et al, 2012;Koskinen et al, 2015).…”

“…Evolutionary adaptation and probiotic potentials of P. freudenreichii strains have previously been assessed by defining the genome sequences of three dairy-associated strains DSM 20271 T , CIRM BIA 1T and JS (Falentin et al, 2010;Koskinen et al, 2015;Ojala et al, 2017). Compared with the genome of the Acidipropionibacterium strain ATCC4875 (Parizzi et al, 2012), the P. freudenreichii strains have smaller genomes (roughly 3.6 Mbp versus 2.6 Mbp), and although many common genes could be identified, the genomes of the two species vary significantly (Parizzi et al, 2012;Koskinen et al, 2015). We recently compared the genomes of 20 P. freudenreichii strains, of which 16 and 4 were from dairy and cereal environments respectively (Deptula et al, 2017b).…”