Complete Genome Sequence of Probiotic Lactobacillus plantarum Strain FMNP01, Isolated from Mango Fruit

Li, Xuefei; Liao, Xueyi; Liu, Yongfeng; Guo, Li‐Qiong; Ye, Zhi‐Wei; Lin, Jizhen

doi:10.1128/genomea.01207-14

Cited by 2 publications

(1 citation statement)

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“…Notably, an isolate of probiotic bacteria of Lactobacillus plantarum has been reported to show an uric acid oxidizing activity (Iswantini et al, 2014). L. plantarum is commonly found in plantderived foods such as fruits, pickles, wine, and bean sauces (Li et al, 2014). It is essential then to explore, select and characterize its uricase in term of both genetic and kinetic properties which is important for developing a potentially probiotic for hyperuricemia.…”

Section: Introductionmentioning

confidence: 99%

Selection of uric acid oxidizing-Lactobacillus plantarum isolates based on their genetic determinant and uricase kinetics

Meriyanti

Artika

Nurhidayat

2020

ijrps

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Hyperuricemia is a condition characterized by abnormally elevated levels of uric acid in the blood. It has been a leading morbidity disease. Microbial uricase can be used to oxidize uric acid into allantoin and hydrogen peroxide in the presence of oxygen and therefore has the potential to play an essential role in reducing uric acid in the people suffering from degenerative disease of hyperuricemia. The present study aims to select uric acid oxidizing-Lactobacillus plantarum isolates based on their genetic determinant and uricase kinetics. A collection of Lactobacillus plantarum isolates were grown on a selective differential medium followed by measuring their uricase activity spectrophotometrically. Specific primers for detection of uricase gene were designed. The uricase coding gene (uox) was then detected in all of the selected isolates by using a qPCR method employing the designed specific primers. The uricase kinetics was determined by the Lineweaver-Burk method. Results showed that all isolates had uricase activity and 4 potential isolates were selected based on their superior uricase activity. The uox gene was detected in all of the selected isolates. The kinetics analysis, however, revealed that only the L. plantarum K-Mar-A2 show strongest substrate affinity and was considered a potential candidate to be developed as a source of therapeutic agent for hyperuricemia.

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Section: Introductionmentioning

confidence: 99%