Complete Genome Sequence of Porcine Deltacoronavirus Strain CH/Sichuan/S27/2012 from Mainland China

Wang, Yiwen; Yue, Hua; Fang, Weihuan; Huang, Yao‐Wei

doi:10.1128/genomea.00945-15

Cited by 53 publications

(60 citation statements)

References 5 publications

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“…The subsequent isolation and pathogenicity of several cell-culture-adapted PDCoV strains (USA/IL/2014, Michigan/8977/ 2014), and Ohio CVM I were also confirmed (Chen et al, 2015a(Chen et al, , 2015bHu et al, 2015;Ma et al, 2015). A histopathological study of naturally PDCoV-infected pigs was consistent with previous pathogenetic studies, and the lesions were similar to but relatively milder than those caused by Porcine epidemic diarrhea virus (PEDV), another enteropathogenic porcine coronavirus (Wang et al, 2015a(Wang et al, , 2015b.…”

Section: Introductionsupporting

confidence: 77%

“…A retrospective epidemiological study showed that PDCoV was detected in pig samples as early as August 2013 in the United States (McCluskey et al, 2015) and in pig samples in South Korea in May 2014 . Some groups have recently reported the emergence of PDCoV in different areas of the Chinese mainland, and interestingly, we detected PDCoV in pig diarrhea samples collected as early as 2004 (Chen et al, 2015a(Chen et al, , 2015bDong et al, 2015;Song et al, 2015;Wang et al, 2015aWang et al, , 2015b. With increased concern about this newly emerging enteric disease, reverse-transcription polymerase chain reaction (RT-PCR), real-time RT-PCR, nested RT-PCR, and singleplex RT insulated isothermal PCR methods have been developed to detect PDCoV in clinical samples Wang et al, 2014a;Song et al, 2015;Zhang et al, 2015Zhang et al, , 2016.…”

Section: Introductionmentioning

confidence: 89%

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Isolation, genomic characterization, and pathogenicity of a Chinese porcine deltacoronavirus strain CHN-HN-2014

Dong

Fang

Yang

et al. 2016

Veterinary Microbiology

111

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Porcine deltacoronavirus (PDCoV) is an emerging swine coronavirus that causes diarrhea in piglets. Since the first outbreak of PDCoV in the United States in 2014, this novel porcine coronavirus has been detected in South Korea, Canada, Mexico, Thailand, and China. In this study, a Chinese PDCoV strain, designated CHN-HN-2014, was isolated from piglets with severe diarrhea on a pig farm in Henan Province, China, and examined with a specific immunofluorescence assay and electron microscopy. Genomic analysis showed that CHN-HN-2014 shares 91.6%-99.4% nucleotide identity with other known PDCoV strains. The pathogenicity of CHN-HN-2014 was further investigated in 5-day-old and 21-day-old piglets. Both kinds of piglets developed clear clinical symptoms, including vomiting, anorexia, lethargy, and severe diarrhea, by 2days postinoculation (DPI), and diarrhea persisted for about 5-6 days. Viral shedding was detected in rectal swabs until 14 DPI in challenged 5-day-old pigs and until 18 DPI in challenged 21-day-old pigs. At necropsy at 4 DPI, macroscopic and microscopic lesions were observed and viral antigen was detected in the small intestines with immunohistochemical staining. These data demonstrate that Chinese PDCoV strain CHN-HN-2014 shares high nucleotide identity with previously reported PDCoV strains and is pathogenic in 5-day-old and 21-day-old piglets.

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Section: Introductionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 89%

Isolation, genomic characterization, and pathogenicity of a Chinese porcine deltacoronavirus strain CHN-HN-2014

Dong

Fang

Yang

et al. 2016

Veterinary Microbiology

111

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“…For example, strain 05 was most closely to KNU14‐04 (S. Korea), OH11846 and Minnesota292 (0.993) (USA). The other strains were most closely to CH/SXD1/2015, Sichuan/S27 and CHN/GX01/2015 (Chen, Gauger et al., ; Chen, Zhu et al., ; Wang, Yue, Fang, & Huang, ; Zhai et al., ) originating in different regions in China (Table S3). The phylogenetic tree of the N gene indicated that strain 05 was located at the US branch, while other positive samples from this work were all clustered in a group within the Chinese branch (Figure ).…”

Section: Resultsmentioning

confidence: 95%

“…The whole S gene (3,483 nt) of published porcine deltacoronavirus isolates was analysed by pairwise alignments. Most of the Chinese strains (HKU15‐155, HB/2014, JS/2014, HN/2014, SXD1, CHJXIN2, GD01/2015, GD02/2015, HN01/2015 and GX01/2015) contained a 3‐nt (AAT) deletion (Chen, Gauger et al., ; Chen, Zhu et al., ; Dong et al., ; Song et al., ; Wang et al., ; Zhai et al., ). In strain CHN/AH/2004 (Dong et al., ), a 3‐nt (TAA) deletion existed in its 3′‐UTR.…”

Section: Discussionmentioning

confidence: 99%

The detection and phylogenetic analysis of porcine deltacoronavirus from Guangdong Province in Southern China

Mai

Feng

Chen

et al. 2017

Transbound Emerg Dis

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Porcine deltacoronavirus (PDCoV) is a newly discovered coronavirus that causes diarrhoea, vomiting and dehydration in sucking and nursing piglets. It was first reported in Hong Kong in 2012 and has since been discovered in the United States, Canada, South Korea, mainland China, Thailand and Laos. PDCoV has been experimentally proved to lead to diarrhoea in swine and it was detected positive in pigs in Guangdong, southern China. In our study, 252 faecal and intestinal samples from sucking piglets and sows with diarrhoea were surveyed for common enteric viruses. We found a prevalence of PDCoV (21.8%), porcine epidemic diarrhoea virus (65.5%), transmissible gastroenteritis virus (0%), rotavirus group A (25.0%) and porcine kobuvirus (68.7%). We isolated 13 PDCoV strains and discovered that PDCoV infections were often co-infections with kobuvirus rather than the commonly linked porcine epidemic diarrhoea virus. Phylogenetic analysis of S gene and N gene revealed that 11 of 13 PDCoV strains belonged to Chinese lineage. As for the left two strains, one single strain (CHN-GD16-05) belonged to American and Korean lineages while another strain (CHN-GD16-03) was similar to a Thai strain, but only in the S gene. This suggested a possible recombination event between the Thai and the newly described Chinese strain.

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“…In 2014, outbreaks of PDCoV were reported in some states of the United States Hu et al, 2015;Marthaler et al, 2014;Wang et al, 2014). Thereafter, PDCoV was also detected in farmed pigs with diarrhea in other countries, including South Korea (Jang et al, 2017;Lee et al, 2016), China (Dong et al, 2015;Song et al, 2015;Wang et al, 2015), Thailand (Janetanakit et al, 2016), Japan (Suzuki et al, 2018), Laos, and Vietnam (Lorsirigool et al, 2016;Saeng-Chuto et al, 2017), causing great economic losses in the swine industry. Recently, Jung and co-workers reported that calves are also susceptible to infection with PDCoV (Jung et al, 2017), sparking an increased interest in studying this emerging virus.…”

Section: Introductionmentioning

confidence: 99%

Porcine deltacoronavirus nsp15 antagonizes interferon-β production independently of its endoribonuclease activity

Liu

Fang

et al. 2019

Molecular Immunology

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Porcine deltacoronavirus (PDCoV) is an emerging swine coronavirus causing diarrhea and intestinal damage in nursing piglets. Previous work showed that PDCoV infection inhibits type I interferon (IFN) production. To further identify and characterize the PDCoV-encoded IFN antagonists will broaden our understanding of its pathogenesis. Nonstructural protein 15 (nsp15) encodes an endoribonuclease that is highly conserved among vertebrate nidoviruses (coronaviruses and arteriviruses) and plays a critical role in viral replication and transcription. Here, we found that PDCoV nsp15 significantly inhibits Sendai virus (SEV)-induced IFN-β production. PDCoV nsp15 disrupts the phosphorylation and nuclear translocation of NF-κB p65 subunit, but not antagonizes the activation of transcription factor IRF3. Interestingly, site-directed mutagenesis found that PDCoV nsp15 mutants (H129A, H234A, K269A) lacking endoribonuclease activity also suppress SEV-induced IFN-β production and NF-κB activation, suggesting that the endoribonuclease activity is not required for its ability to antagonize IFN-β production. Taken together, our results demonstrate that PDCoV nsp15 is an IFN antagonist and it inhibits interferon-β production via an endoribonuclease activity-independent mechanism.

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Complete Genome Sequence of Porcine Deltacoronavirus Strain CH/Sichuan/S27/2012 from Mainland China

Cited by 53 publications

References 5 publications

Isolation, genomic characterization, and pathogenicity of a Chinese porcine deltacoronavirus strain CHN-HN-2014

Isolation, genomic characterization, and pathogenicity of a Chinese porcine deltacoronavirus strain CHN-HN-2014

The detection and phylogenetic analysis of porcine deltacoronavirus from Guangdong Province in Southern China

Porcine deltacoronavirus nsp15 antagonizes interferon-β production independently of its endoribonuclease activity

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