Complete genome sequence of Planococcus faecalis AJ003 T , the type species of the genus Planococcus and a microbial C30 carotenoid producer

Kim, Jin Won; Choi, Bo Hyun; Kim, Jin Ho; Kang, Ho Jung; Ryu, Hoon; Lee, Pyung Cheon

doi:10.1016/j.jbiotec.2017.12.005

Cited by 11 publications

(9 citation statements)

References 26 publications

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“…The whole-genome sequences of several kinds of Planococcus were available in the genome database ( https://www.ncbi.nlm.nih.gov/genome/ ). All of them, including P. faecalis AJ003 T , had identical carotenoid biosynthesis gene clusters to that found in this study [ 19 , 23 ]. Similar gene clusters have also been reported in Halobacillus halophilus , Staphylococcus aureus , Bacillus indicus , and Bacillus firmus , which all belong to the order of Bacillales (Fig.…”

Section: Discussionsupporting

confidence: 58%

“…Its yellow pigment was identified as methyl 5-glucosyl-5,6-dihydro-4,4′-diapolycopenoate (methyl 5-glucosyl-5,6-dihydro-apo-4,4′-lycopenoate), which showed potent antioxidative activity [ 8 , 9 ]. Kim et al [ 23 ] also reported that Planococcus faecalis AJ003 T produced a similar C 30 -carotenoid glycosyl-4,4′-diaponeurosporen-4′-ol-4-oate.…”

Section: Introductionmentioning

confidence: 99%

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Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes

et al. 2021

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Background Members of the genus Planococcus have been revealed to utilize and degrade solvents such as aromatic hydrocarbons and alkanes, and likely to acquire tolerance to solvents. A yellow marine bacterium Planococcus maritimus strain iso-3 was isolated from an intertidal sediment that looked industrially polluted, from the Clyde estuary in the UK. This bacterium was found to produce a yellow acyclic carotenoid with a basic carbon 30 (C30) structure, which was determined to be methyl 5-glucosyl-5,6-dihydro-4,4′-diapolycopenoate. In the present study, we tried to isolate and identify genes involved in carotenoid biosynthesis from this marine bacterium, and to produce novel or rare C30-carotenoids with anti-oxidative activity in Escherichia coli by combinations of the isolated genes. Results A carotenoid biosynthesis gene cluster was found out through sequence analysis of the P. maritimus genomic DNA. This cluster consisted of seven carotenoid biosynthesis candidate genes (orf1–7). Then, we isolated the individual genes and analyzed the functions of these genes by expressing them in E. coli. The results indicated that orf2 and orf1 encoded 4,4′-diapophytoene synthase (CrtM) and 4,4′-diapophytoene desaturase (CrtNa), respectively. Furthermore, orf4 and orf5 were revealed to code for hydroxydiaponeurosporene desaturase (CrtNb) and glucosyltransferase (GT), respectively. By utilizing these carotenoid biosynthesis genes, we produced five intermediate C30-carotenoids. Their structural determination showed that two of them were novel compounds, 5-hydroxy-5,6-dihydro-4,4′-diaponeurosporene and 5-glucosyl-5,6-dihydro-4,4′-diapolycopene, and that one rare carotenoid 5-hydroxy-5,6-dihydro-4,4′-diapolycopene is included there. Moderate singlet oxygen-quenching activities were observed in the five C30-carotenoids including the two novel and one rare compounds. Conclusions The carotenoid biosynthesis genes from P. maritimus strain iso-3, were isolated and functionally identified. Furthermore, we were able to produce two novel and one rare C30-carotenoids in E. coli, followed by positive evaluations of their singlet oxygen-quenching activities.

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Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes

et al. 2021

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“…Studying G. ajoucoccus A2—including whole-genome sequencing—to validate it as a carotenoid producer is worthwhile. These genomic data can form the basis for elucidation of carotenoid biosynthesis mechanisms and for metabolic engineering of Gordonia strains ( 5 – 8 ) suitable for large-scale fermentative production of other biotechnologically important carotenoids from industrial discharge or other sources of n -alkanes.…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of the Carotenoid-Producing Strain Gordonia ajoucoccus A2

Kwon

Choi

Kim

et al. 2020

Microbiol Resour Announc

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“…strain AJ005 in detail, including whole-genome sequencing, in order to validate it as a potential carotenoid producer would be valuable. This genomic information provides a basis for developing recombinant Deinococcus strains and for elucidating carotenoid production mechanisms (4, 5) suitable for the large-scale fermentation of other biotechnologically important carotenoids.…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of the Carotenoid-Producing Deinococcus sp. Strain AJ005

Choi

Lee

2019

Microbiol Resour Announc

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Complete genome sequence of Planococcus faecalis AJ003 T , the type species of the genus Planococcus and a microbial C30 carotenoid producer

Cited by 11 publications

References 26 publications

Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes

Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes

Complete Genome Sequence of the Carotenoid-Producing Strain Gordonia ajoucoccus A2

Complete Genome Sequence of the Carotenoid-Producing Deinococcus sp. Strain AJ005

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