Complete Genome Sequence of Phytopathogenic Pectobacterium carotovorum subsp. carotovorum Bacteriophage PP1

Lee, Ju-Hoon; Shin, Hakdong; Ji, Samnyu; Malhotra, Shweta; Kumar, Mukesh; Ryu, Sangryeol; Heu, Sunggi

doi:10.1128/jvi.01283-12

Cited by 27 publications

(14 citation statements)

References 12 publications

(8 reference statements)

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“…PP1 and PPWS1 infecting P. carotovorum subsp. carotovorum (Lee et al 2012;Hirata et al 2016) and Peat1 and ϕM1 infecting P. atrosepticum (Kalischuk et al 2015;Blower et al 2017) were classified to Podoviridae family and neither of those infects specifically P. parmentieri. This may suggest that the SRE-infecting Podoviridae bacteriophages are difficult to isolate using standard procedures and/or that the Podoviridae phages are rare in the environment (Ackermann 2011).…”

Section: Discussionmentioning

confidence: 99%

Isolation and phenotypic and morphological characterization of the first Podoviridae lytic bacteriophages ϕA38 and ϕA41 infecting Pectobacterium parmentieri (former Pectobacterium wasabiae)

et al. 2017

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Two bacteriophages, ϕA38 and ϕA41, infecting Pectobacterium parmentieri strain SCC 3193 (former Pectobacterium wasabiae strain SCC 3193) were isolated from arable soil samples collected in different regions of Poland. ϕA38 and ϕA41 have a typical morphology of the members of the family Podoviride and order Caudovirales, with a head diameter of ca. 60 nm and tail length of ca. 20 nm. Phages ϕA38 and ϕA41 exhibited a similar RFLP pattern with Csp6I restriction endonuclease. They were stable in a range of pHs, temperatures and osmolarities but were rapidly inactivated by UV light. During the first 20 min., 74 and 69% of ϕA38 and ϕA41 phages, respectively, were adsorbed to SCC 3193 cells. In one-step growth experiments, ϕA38 and ϕA41 showed latent period of ca. 20-30 min and burst size of 102 and 141 phages, respectively. The optimal multiplicity of infection (MOI) was calculated to be 0.01 for both bacteriophages. In the host range experiments, both phages were able to infect six from 21 of the tested P. parmentieri isolates but the phages were unable to infect other members of the Pectobacterium spp. or Dickeya spp. In the proof-of-concept experiments, ϕA38 and ϕA41 were able to inhibit the growth of P. parmentieri strain SCC 3193 and to protect potato tuber tissue maceration caused by the bacterium. The potential use of ϕA38 and ϕA41 bacteriophages for the biocontrol of P. parmentieri in potato is discussed.

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Section: Discussionmentioning

confidence: 99%

Isolation and phenotypic and morphological characterization of the first Podoviridae lytic bacteriophages ϕA38 and ϕA41 infecting Pectobacterium parmentieri (former Pectobacterium wasabiae)

et al. 2017

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“…The only bacteriophage with noticeable homology in the genome sequence is the Escherichia phage ECBP5 (KJ749827), genetically assessed as a mosaic composition between various Autographivirinae phages ; Figure 4A). Phylogenetic trees generated using concatenated alignments of amino acid sequences of DNA polymerase, a major capsid protein and a terminase large subunit show the relationship between PP99 and Salmonella phage SP6 (NCBI accession number AY370673) (Dobbins et al, 2004), Pectobacterium phage PP1 (NC_019542) (Lee et al, 2012), Vibrio phage ϕA318 (KF322026) (Liu et al, 2014), and a number of other Podoviruses infecting a broad range of bacteria (Figures S2, S3). Thus, PP99 can be assigned as a member of the genus SP6virus.…”

Section: Bacteriophage Pp99 Genome Featuresmentioning

confidence: 99%

Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection

et al. 2020

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Soft rot caused by numerous species of Pectobacterium and Dickeya is a serious threat to the world production of potatoes. The application of bacteriophages to combat bacterial infections in medicine, agriculture, and the food industry requires the selection of comprehensively studied lytic phages and the knowledge of their infection mechanism for more rational composition of therapeutic cocktails. We present the study of two bacteriophages, infective for the Pectobacterium brasiliense strain F152. Podoviridae PP99 is a representative of the genus Zindervirus, and Myoviridae PP101 belongs to the still unclassified genomic group. The structure of O-polysaccharide of F152 was established by sugar analysis and 1D and 2D NMR spectroscopy:The recombinant tail spike protein of phage PP99, gp55, was shown to deacetylate the side chain talose residue of bacterial O-polysaccharide, thus providing the selective attachment of the phage to the cell surface. Both phages demonstrate lytic behavior, thus being prospective for therapeutic purposes.

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“…1). The full genome of bacteriophage PP1 was sequenced [22]. The genome of PP1 was of 44,400 bp with a GC content of 49.74%.…”

Section: Isolation Of Bacteriophage Pp1mentioning

confidence: 99%

Biocontrol of Pectobacterium carotovorum subsp. carotovorum Using Bacteriophage PP1

Lim

Jee²,

Lee³

et al. 2013

J. Microbiol. Biotechnol.

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Pectobacterium carotovorum subsp. carotovorum (formerly Erwinia carotovora subsp. carotovora) is a plant pathogen that causes soft rot and stem rot diseases in several crops, including Chinese cabbage, potato, and tomato. To control this bacterium, we isolated a bacteriophage, PP1, with lytic activity against P. carotovorum subsp. carotovorum. Transmission electron microscopy revealed that the PP1 phage belongs to the Podoviridae family of the order Caudovirales, which exhibit icosahedral heads and short non-contractile tails. PP1 phage showed high specificity for P. carotovorum subsp. carotovorum, and several bacteria belonging to different species and phyla were resistant to PP1. This phage showed rapid and strong lytic activity against its host bacteria in liquid medium and was stable over a broad range of pH values. Disease caused by P. carotovorum subsp. carotovorum was significantly reduced by PP1 treatment. Overall, PP1 bacteriophage effectively controls P. carotovorum subsp. carotovorum.

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Complete Genome Sequence of Phytopathogenic Pectobacterium carotovorum subsp. carotovorum Bacteriophage PP1

Cited by 27 publications

References 12 publications

Isolation and phenotypic and morphological characterization of the first Podoviridae lytic bacteriophages ϕA38 and ϕA41 infecting Pectobacterium parmentieri (former Pectobacterium wasabiae)

Isolation and phenotypic and morphological characterization of the first Podoviridae lytic bacteriophages ϕA38 and ϕA41 infecting Pectobacterium parmentieri (former Pectobacterium wasabiae)

Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection

Biocontrol of Pectobacterium carotovorum subsp. carotovorum Using Bacteriophage PP1

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