Complete Genome Sequence of Peptacetobacter (Clostridium) hiranonis Strain DGF055142, Isolated from Dog Feces from Flagstaff, Arizona, USA, 2019

Stone, Nathan E.; Nunnally, Amalee E.; Roe, Chandler C.; Hornstra, Heidie; Wagner, David M.; Sahl, Jason W.

doi:10.1128/mra.00067-21

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…31–33 Further analysis of the currently available P. hiranonis genome sequences (one each of human and dog origin) in GenBank resulted in the same observation of bai and bsh presence in those strains. 29, 34…”

Section: Resultsmentioning

confidence: 99%

“…[31][32][33] Further analysis of the currently available P. hiranonis genome sequences (one each of human and dog origin) in GenBank resulted in the same observation of bai and bsh presence in those strains. 29,34 Having both bsh gene and bai operon in a single bacterium has immense therapeutic significance. Such bacteria could perform the functions of two different groups of metabolically dependant commensal bacteria in a dysbiotic gut of a CDI patient to restore normal bile acid metabolism.…”

Section: P Hiranonis Bai-17 Possesses a Unique Combination Of Both Bsh And Bai Genes With The Absence Of Known Toxin Genesmentioning

confidence: 99%

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A model screening pipeline for bile acid converting anti-Clostridioides difficile bacteria reveals unique biotherapeutic potential of Peptacetobacter hiranonis

Aa¹,

Bg²,

Ghimire

et al. 2021

Preprint

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Clostridioides difficile is an antibiotic-resistant bacterium that causes serious, toxin-mediated enteric disease in humans and animals. Gut dysbiosis and resultant alterations in the intestinal bile acid profile play an important role in the pathogenesis of C. difficile infection (CDI). Restoration of the gut microbiota and re-establishment of bacterial bile acid metabolism using fecal microbiota transplantation (FMT) has been established as a promising strategy against this disease, although this method has several limitations. Thus, a more defined and precise microbiota-based approach using bacteria that biotransform primary bile acids into secondary bile acids could effectively overcome these limitations and control CDI. Therefore, a screening pipeline was developed to isolate bile acid converting bacteria from fecal samples. Dogs were selected as a model CDI-resistant microbiota donor for this pipeline, which yielded a novel Peptacetobacter hiranonis strain that possesses unique anti-C. difficile properties, and both bile acid deconjugation and 7-α dehydroxylating activities to perform bile acid conversion. The screening pipeline included a set of in vitro tests along with a precision in vivo gut colonization and bile acid conversion test using altered Schadler flora (ASF) colonized mice. In addition, this pipeline also provided essential information on the growth requirements for screening and cultivating the candidate bacterium, its survival in a CDI predisposing environment, and potential pathogenicity. The model pipeline documented here yielded multiple bile acid converting bacteria, including a P. hiranonis isolate with unique anti-C. difficile biotherapeutic potential, which can be further tested in subsequent preclinical and human clinical trials.

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Section: Resultsmentioning

confidence: 99%

Section: P Hiranonis Bai-17 Possesses a Unique Combination Of Both Bsh And Bai Genes With The Absence Of Known Toxin Genesmentioning

confidence: 99%

A model screening pipeline for bile acid converting anti-Clostridioides difficile bacteria reveals unique biotherapeutic potential of Peptacetobacter hiranonis

Aa¹,

Bg²,

Ghimire

et al. 2021

Preprint

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show abstract

“…This organism is considered a key feature of the normal, healthy gut microbiome of dogs [ 22 ]. A full genome from P. hiranonis has been characterized from a canine fecal isolate [ 51 ] and the complete bai operon identified, confirming the genetic capability to perform 7α-dehydroxylation [ 52 ]. Further, the absence of P. hiranonis from the gut microbiome is an indicator associated with dysbiotic states in dogs [ 53 ] and cats [ 54 ].…”

Section: Collaborative Physiology Of Bile Acid Metabolismmentioning

confidence: 99%

Collaborative Metabolism: Gut Microbes Play a Key Role in Canine and Feline Bile Acid Metabolism

Rowe,

Winston

2024

Veterinary Sciences

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Bile acids, produced by the liver and secreted into the gastrointestinal tract, are dynamic molecules capable of impacting the overall health of dogs and cats in many contexts. Importantly, the gut microbiota metabolizes host primary bile acids into chemically distinct secondary bile acids. This review explores the emergence of new literature connecting microbial-derived bile acid metabolism to canine and feline health and disease. Moreover, this review highlights multi-omic methodologies for translational research as an area for continued growth in veterinary medicine aimed at accelerating microbiome science and medicine as it pertains to bile acid metabolism in dogs and cats.

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Diet-induced changes in fecal microbiota composition and diversity in dogs (Canis lupus familiaris): A comparative study of BARF-type and commercial diets

Castañeda,

Ariza,

Rincón-Riveros

et al. 2023

Comparative Immunology, Microbiology and Infectious Diseases

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Complete Genome Sequence of Peptacetobacter (Clostridium) hiranonis Strain DGF055142, Isolated from Dog Feces from Flagstaff, Arizona, USA, 2019

Cited by 3 publications

References 18 publications

A model screening pipeline for bile acid converting anti-Clostridioides difficile bacteria reveals unique biotherapeutic potential of Peptacetobacter hiranonis

A model screening pipeline for bile acid converting anti-Clostridioides difficile bacteria reveals unique biotherapeutic potential of Peptacetobacter hiranonis

Collaborative Metabolism: Gut Microbes Play a Key Role in Canine and Feline Bile Acid Metabolism

Diet-induced changes in fecal microbiota composition and diversity in dogs (Canis lupus familiaris): A comparative study of BARF-type and commercial diets

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