Complete Genome Sequence of Pelagibacterium halotolerans B2T

Huo, Ying‐Yi; Cheng, Hong; Han, Xifang; Jiang, Xin; Sun, Cong; Zhang, Xin-Qi; Zhu, Xuemei; Liu, Yongfeng; Li, Pengfei; Ni, Peixiang; Wu, Min

doi:10.1128/jb.06343-11

Cited by 20 publications

(6 citation statements)

References 14 publications

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“…1 of their Other dominant taxa in C. australiensis hosts included OTU-176 (family: Hyphomicrobiaceae) and OTU-1769 (family: Rhodobacteraceae), both restricted to lake habitat. The Hyphomicrobiaceae are ubiquitous in terrestrial and freshwater habitats, but only a few have been recorded from marine environments (Huo et al, 2012). Species belonging to the Hyphomicrobiaceae have been identified as important methylotrophic denitrifiers (Liessens et al, 1993;Osaka et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Habitat- and host-related variation in sponge bacterial symbiont communities in Indonesian waters

Cleary

Becking

Voogd

et al. 2013

FEMS Microbiol Ecol

100

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Marine lakes are unique ecosystems that contain isolated populations of marine organisms. Isolated from the surrounding marine habitat, many lakes house numerous endemic species. In this study, microbial communities of sponges inhabiting these lakes were investigated for the first time using barcoded pyrosequencing of 16S rRNA gene amplicons. Our main goals were to compare the bacterial richness and composition of two sponge species (Suberites diversicolor and Cinachyrella australiensis) inhabiting both marine lakes and adjacent open coastal systems. Host species and habitat explained almost 59% of the variation in bacterial composition. There was a significant difference in composition between both host species. Within S. diversicolor, there was little discernible difference between bacterial communities inside and outside lakes. The bacterial community of this species was, furthermore, dominated (63% of all sequences) by three very closely related alphaproteobacterial taxa identified as belonging to the recently described order Kiloniellales. Cinachyrella australiensis, in contrast, hosted markedly different bacterial communities inside and outside lakes with very few shared abundant taxa. Cinachyrella australiensis in open habitat only shared 9.4% of OTUs with C. australiensis in lake habitat. Bacteria were thus both highly species specific and, in the case of C. australiensis, habitat specific.

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Section: Discussionmentioning

confidence: 99%

Habitat- and host-related variation in sponge bacterial symbiont communities in Indonesian waters

Cleary

Becking

Voogd

et al. 2013

FEMS Microbiol Ecol

100

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“…A particularity of the “ Ca . P. ectocarpi” genome is that, unlike many Alphaproteobacteria (Data sheet 4 ), it also encodes all enzymes required to recycle trehalose (GT20, GH15, trehalose-6-phosphatase) (Brown et al, 2011 ; Schleheck et al, 2011 ; Huo et al, 2012 ; Martineau et al, 2013 ). This indicates that “ Ca .…”

Section: Discussionmentioning

confidence: 99%

Genome and metabolic network of â€œCandidatus Phaeomarinobacter ectocarpiâ€ Ec32, a new candidate genus of Alphaproteobacteria frequently associated with brown algae

et al. 2014

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Rhizobiales and related orders of Alphaproteobacteria comprise several genera of nodule-inducing symbiotic bacteria associated with plant roots. Here we describe the genome and the metabolic network of “Candidatus Phaeomarinobacter ectocarpi” Ec32, a member of a new candidate genus closely related to Rhizobiales and found in association with cultures of the filamentous brown algal model Ectocarpus. The “Ca. P. ectocarpi” genome encodes numerous metabolic pathways that may be relevant for this bacterium to interact with algae. Notably, it possesses a large set of glycoside hydrolases and transporters, which may serve to process and assimilate algal metabolites. It also harbors several proteins likely to be involved in the synthesis of algal hormones such as auxins and cytokinins, as well as the vitamins pyridoxine, biotin, and thiamine. As of today, “Ca. P. ectocarpi” has not been successfully cultured, and identical 16S rDNA sequences have been found exclusively associated with Ectocarpus. However, related sequences (≥97% identity) have also been detected free-living and in a Fucus vesiculosus microbiome barcoding project, indicating that the candidate genus “Phaeomarinobacter” may comprise several species, which may colonize different niches.

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“…The Pelagibacterium halotolerans B2 T genome was sequenced by the whole-genome sequencing with highthroughput technologies (Huo et al 2012). We found a putative esterase gene (PE10), and the deduced sequence of amino acids was analyzed using blastp program (http:// blast.ncbi.nlm.nih.gov/).…”

Section: Sequence Analysismentioning

confidence: 99%

Cloning, expression and characterization of a halotolerant esterase from a marine bacterium Pelagibacterium halotolerans B2T

et al. 2012

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An esterase PE10 (279 aa) from Pelagibacterium halotolerans B2(T) was cloned and overexpressed in Escherichia coli Rosetta in a soluble form. The deduced protein was 29.91 kDa and the phylogenetic analysis of the deduced amino acids sequence showed it represented a new family of lipolytic enzymes. The recombinant protein was purified by Ni-NTA affinity chromatography column and the characterization showed its optimal temperature and pH were 45 °C and pH 7.5, respectively. Substrate specificity study showed PE10 preferred short chain p-nitrophenyl esters and exhibited maximum activity toward p-nitrophenyl acetate. In addition, PE10 was a halotolerant esterase as it was still active under 4 M NaCl. Three-dimensional modeling of PE10 suggested that the high negative electrostatic potential on the surface may relevant to its tolerance to high salt environment. With this halotolerance property, PE10 could be a candidate for industrial use.

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Complete Genome Sequence of Pelagibacterium halotolerans B2T

Cited by 20 publications

References 14 publications

Habitat- and host-related variation in sponge bacterial symbiont communities in Indonesian waters

Habitat- and host-related variation in sponge bacterial symbiont communities in Indonesian waters

Genome and metabolic network of â€œCandidatus Phaeomarinobacter ectocarpiâ€ Ec32, a new candidate genus of Alphaproteobacteria frequently associated with brown algae

Cloning, expression and characterization of a halotolerant esterase from a marine bacterium Pelagibacterium halotolerans B2T

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Complete Genome Sequence of Pelagibacterium halotolerans B2T

Cited by 20 publications

References 14 publications

Habitat- and host-related variation in sponge bacterial symbiont communities in Indonesian waters

Habitat- and host-related variation in sponge bacterial symbiont communities in Indonesian waters

Genome and metabolic network of â€œCandidatus Phaeomarinobacter ectocarpiâ€ Ec32, a new candidate genus of Alphaproteobacteria frequently associated with brown algae

Cloning, expression and characterization of a halotolerant esterase from a marine bacterium Pelagibacterium halotolerans B2T

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Genome and metabolic network of â€œCandidatus Phaeomarinobacter ectocarpiâ€ Ec32, a new candidate genus of Alphaproteobacteria frequently associated with brown algae