Complete Genome Sequence of pAP13, a Large Linear Plasmid of a
            <i>Brevibacterium</i>
            Strain Isolated from a Saline Lake at 4,200 Meters above Sea Level in Argentina

Dib, Julián Rafael; Schuldes, Jörg; Thürmer, Andrea; Farı́as, Marı́a Eugenia; Daniel, Rolf; Meinhardt, Friedhelm

doi:10.1128/genomea.00878-13

Cited by 7 publications

(6 citation statements)

References 6 publications

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“…The strains were taxonomically identified and characterized in terms of their resistance to environmental conditions such as arsenic, salt, UV and antibiotics (3)(4)(5)14,16,41,46,47), indicating that HAAL constitute an important reserve of genes and molecular mechanisms to counteract various stress factors. In the light of ongoing genomic/ metagenomic projects (19,21,22,(24)(25)(26)(27)(28)72), putative extremoenzymes and extremolytes produced by these microbes offer promising biotechnological applications that range from bioremediation and biomining to the production of important drugs.…”

Section: Polyextremophiles: When One Extreme Is Not Enoughmentioning

confidence: 99%

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Forged Under the Sun: Life and Art of Extremophiles from Andean Lakes

Albarracín

Gärtner

Farı́as

2015

Photochem & Photobiology

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High-altitude Andean lakes (HAAL) are a treasure chest for microbiological research in South America. Their indigenous microbial communities are exposed to extremely high UV irradiation and to multiple chemical extremes (Arsenic, high salt content, alkalinity). Microbes are found both, free-living or associated into microbial mats with different degrees of mineralization and lithification, including unique modern stromatolites located at 3570 m above sea level. Characterization of these polyextremophilic microbes began only recently, employing morphological and phylogenetic methods as well as high-throughput sequencing and proteomics approach. Aside from providing a general overview on microbial communities, special attention is given to various survival strategies; HAAL's microbes present a complex system of shared genetic and physiological mechanisms (UV-resistome) based on UV photoreceptors and stress sensors with their corresponding response regulators, UV avoidance and protection strategies, damage tolerance and UV damage repair. Molecular information will be provided for what is, so far the most studied HAAL molecule, a CPD-Class I photolyase from Acinetobacter Ver3 (Laguna Verde, 4400 m). This work further proposes some strategies that make an appeal for the preservation of HAAL, a highly fragile environment that offers promising and ample research possibilities.

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Section: Polyextremophiles: When One Extreme Is Not Enoughmentioning

confidence: 99%

“…A1, L. Azul, planktonic), pJD12 (KR152226, Micrococcus sp. D12, red biofilm, L. Diamante) (19,21,22,(24)(25)(26)(27)(28)72). Meanwhile, metagenomic shotgun sequencing is in progress.…”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Forged Under the Sun: Life and Art of Extremophiles from Andean Lakes

Albarracín

Gärtner

Farı́as

2015

Photochem & Photobiology

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“…Continuing that idea, we have previously demonstrated that water in high-irradiated pristine environments was a source for isolating bacteria able to grow in the presence of antibiotics, and that the bacteria were also present in flamingos' enteric biota, probably taken from the water where they feed [3]. In addition, we have found that several isolated bacteria present giant extra chromosomal linear elements, the so-called linear plasmids [21][22][23]. We found that the presence of linear plasmids might be related to the antibiotics-resistant dispersion.…”

Section: Discussionmentioning

confidence: 83%

Water and Flamingo Feces Bacterial Communities from High- Altitude Andean Lakes under Selective Antibiotic Pressure Studied by PCR-DGGE Analyses

Fernández-Zenoff¹,

Dib²,

Neumann³

et al. 2015

Antimicrobial Resistance - An Open Challenge

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High-altitude "ndean lakes are exposed to extreme environmental factors like high salinity, ultraviolet radiation, heavy metals, among others. "s it was previously shown, these lakes are not only the habitat of a high diversity of bacteria with multiple resistances they also support an enormous population of flamingos, which migrate among these wetlands, and they could play a role as disseminators and/or reservoirs of pathogenic bacteria.The aim of this work was to analyze, by Denaturant Gradient Gel Electrophoresis, the bacterial population under selective antibiotic pressure from bacterioplankton and flamingo feces from three lakes, placed between altitudes , and , m. "lmost all bands were present in antibiotic-enriched cultures. Several bands identified in water were found in feces as well, presenting mainly correspondence with Gamma-proteobacteria. Few bands were exclusively identified in water, and those presenting correspondence with Alfa-proteobacteria and "ctinobacteria were only identified in Laguna "zul. This study established that flamingos' enteric biota is in close interaction with lake water and demonstrated that bacteria with the ability to grow in antibiotics are abundant and diverse in the microbiota of "ndean lakes. "dditionally, flamingos could be considered as vectors of pathogenic organisms, since Stenotrophomonas seem to be the widest spread bacteria in the studied lakes.

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“…Among bacteria, linear elements are found in Gram-negative and -positive species (reviewed in Hinnebusch and Tilly, 1993 ; Meinhardt and Klassen, 2007 ). The majority, however, is found in the latter group, particularly in Actinomycetes including the genera Streptomyces , Rhodococcus , Micrococcus , and Brevibacterium ( Dib et al, 2010a , b , 2013a , b , c ). pSLA2 of Streptomyces rochei represents the first bacterial linear plasmid that was described ( Hayakawa et al, 1979 ).…”

Section: Linear Plasmidsmentioning

confidence: 99%

“…Thus, even though a panoply of linear plasmids from isolates originating from diverse environments have been fully sequenced and genetically characterized ( Le Dantec et al, 2001 ; Stecker et al, 2003 ; Bentley et al, 2004 ; Parschat et al, 2007 ; Dib et al, 2013b , c ; Miller et al, 2013 ), the ecological impact of such accessory genetic elements as a whole remains largely obscure.…”

Section: Linear Plasmids and The Plasmidomementioning

confidence: 99%

Strategies and approaches in plasmidome studiesâ€”uncovering plasmid diversity disregarding of linear elements?

Dib

Wagenknecht

Farı́as³

et al. 2015

Front. Microbiol.

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The term plasmid was originally coined for circular, extrachromosomal genetic elements. Today, plasmids are widely recognized not only as important factors facilitating genome restructuring but also as vehicles for the dissemination of beneficial characters within bacterial communities. Plasmid diversity has been uncovered by means of culture-dependent or -independent approaches, such as endogenous or exogenous plasmid isolation as well as PCR-based detection or transposon-aided capture, respectively. High-throughput-sequencing made possible to cover total plasmid populations in a given environment, i.e., the plasmidome, and allowed to address the quality and significance of self-replicating genetic elements. Since such efforts were and still are rather restricted to circular molecules, here we put equal emphasis on the linear plasmids which—despite their frequent occurrence in a large number of bacteria—are largely neglected in prevalent plasmidome conceptions.

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Complete Genome Sequence of pAP13, a Large Linear Plasmid of a Brevibacterium Strain Isolated from a Saline Lake at 4,200 Meters above Sea Level in Argentina

Cited by 7 publications

References 6 publications

Forged Under the Sun: Life and Art of Extremophiles from Andean Lakes

Forged Under the Sun: Life and Art of Extremophiles from Andean Lakes

Water and Flamingo Feces Bacterial Communities from High- Altitude Andean Lakes under Selective Antibiotic Pressure Studied by PCR-DGGE Analyses

Strategies and approaches in plasmidome studiesâ€”uncovering plasmid diversity disregarding of linear elements?

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