Complete genome sequence of Paenibacillus konkukensis sp. nov. SK3146 as a potential probiotic strain

Abstract

Paenibacillus konkukensis sp. nov., SK3146 is a novel strain isolated from a pig feed. Here, we present complete genome sequence of SK3146. The genome consists of a single circular genome measuring 7,968,964 bp in size with an average guanine + cytosine (G+C) content of 53.4%. Genomic annotation revealed that the strain encodes 151 proteins related to hydrolases (EC3), which was higher than those in Bacillus subtilis and Escherichia coli . Diverse kinds of hydrolases including galactosidase, glucosidase, cellulase, lipase, xylanase, and protease were found in the genome of SK3146, coupled with one bacteriocin encoding gene. The complete genome sequence of P. konkukensis SK3146 indicates the immense probiotic potential of the strain with nutrient digestibility and antimicrobial activity functions.

Phenotypic and Draft Genome Sequence Analyses of a Paenibacillus sp. Isolated from the Gastrointestinal Tract of a North American Gray Wolf (Canis lupus)

“…For monogastric animals, a P. xylanexedens strain reportedly improved broiler performance by impacting intestinal morphology, enhanced the overall immune response and reduced E. coli in the cecum [45]. Also, a P. konkukensis sp., nov. SK3146, has been proposed as a probiotic for swine [46]. For example, other Paenibacillus spp.…”

Potential Probiotic Acceptability of a Novel Strain of Paenibacillus konkukensis SK 3146 and Its Dietary Effects on Growth Performance, Intestinal Microbiota, and Meat Quality in Broilers

“…In addition, probiotic feeding of P. xylanexedens , which secretes enzymes such as D-cellulose, D-fructose, D-galactose, α-D-glucose, lactose, and maltose as actively as Bacillus species, improved the BW gain and FCR in broilers under normal feeding environment [ 17 ] or challenged conditions [ 18 ]. Similarly, P. konkukensis , isolated and analyzed for complete genome sequence in our previous studies, has DNA sequences encoding various types of enzymes such as β-glucosidase, cellulase, xylanase, and protease, which can degrade various carbohydrates and anti-nutritional factors [ 23 , 24 ]. However, in this study, P. konkukensis had no measured effects on the growth parameters.…”

“…In studies including Paenibacillus strains, P. polymyxa with antibacterial activity against pathogenic bacterial strains reduced the E. coli and Enterococcus spp. in cecal microbiota of Japanese quail [ 20 ] and decreased ileal Enterobacteriaceae counts in broilers when the strain was fed in fermented form with palm kernel cake [ 24 ]. Another study reported that P. xylanexedens ysm 1 with antibacterial activity against E. coli reduced the cecal E. coli in broilers challenged with E. coli K88 [ 18 ].…”

“…In our previous studies [ 23 , 24 ], P. konkukensis sp. nov. SK-3146 T (=KACC 18876 T = LMG 29568 T ) was isolated from animal feed and completely sequenced.…”

Perspectives of Probiotics-based Cheese Research and Its Industrial Development

“…이들은 식품 내에서 공간 및 영양소를 차지하기 유해 미생물의 번 식을 억제하고 식품의 유해 미생물 오염을 예방할 수 있다. 또한 프로바이오틱스는 식품의 pH 값을 조절하여 식품의 산도를 유지하거나 조절하며, 발효 과정을 통해 유익한 활성 물질을 생성하여 식품 내 유해 성분을 분해하거나 억제하고, 식품의 맛, 향, 질감을 개선하기도 한다 [11][12][13][14].…”