Complete Genome Sequence of Mycobacterium chelonae Type Strain CCUG 47445, a Rapidly Growing Species of Nontuberculous Mycobacteria

Jaén‐Luchoro, Daniel; Salvà‐Serra, Francisco; Aliaga-Lozano, Francisco; Seguí, Carolina; Busquets, Antonio; Ramírez, Antonio; Ruiz, M.; Gomila, Margarita; Lalucat, Jorge; Bennasar, Antonio

doi:10.1128/genomea.00550-16

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…Parenteral antibiotics against Mycobacterium chelonae include tobramycin, amikacin, imipenem, and tigecycline, but it has demonstrated resistance to antibiotics and disinfectants (Brown-Elliott et al, 2012;Jaén-Luchoro et al, 2016). This property assists Mycobacterium chelonae in colonizing water systems and allows its access to humans (Jaén-Luchoro et al, 2016). Till now, no specific guidelines for the treatment of Mycobacterium chelonae have been defined in the literature (Gonzalez-Santiago and Drage, 2015).…”

Section: Introductionmentioning

confidence: 99%

Drug Target Identification and Virtual Screening in Pursuit of Phytochemical Intervention ofMycobacterium chelonae

Basharat

Zaib

Yasmin

et al. 2018

Preprint

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Mycobacterium chelonae is a rapidly growing mycobacterium present in the environment. It is associated with skin and soft tissue infections including abscess, cellulitis and osteomyelitis. Other infections by this bacterium are post-operative/transplant-associated, catheter, prostheses and even concomitant to haemodialytic procedures. In this study, we employ a subtractive genomics approach to predict the potential therapeutic candidates, intended for experimental research against this bacterium. A computational workflow was devised and executed to procure core proteome targets essential to the pathogen but with no similarity to the human host. Initially, essential Mycobacterium chelonae proteins were predicted through homology searching of core proteome content from 19 different bacteria. Druggable proteins were then identified and Nacetylglucosamine-1-phosphate uridyltransferase (GlmU) was chosen as a case study from identified therapeutic targets, based on its important bifunctional role. Structure modeling was followed by virtual screening of phytochemical library (N > 2200), from 500 medicinal plants, against it. A biflavonoid daphnodorin G from Daphne odora was screened as having best potential for binding GlmU. Phytotherapy helps curb the menace of antibiotic resistance so treatment of Mycobacterium chelonae infection through this method is recommended.

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Section: Introductionmentioning

confidence: 99%

Drug Target Identification and Virtual Screening in Pursuit of Phytochemical Intervention ofMycobacterium chelonae

Basharat

Zaib

Yasmin

et al. 2018

Preprint

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Genome-Wide Essentiality Analysis of Mycobacterium abscessus by Saturated Transposon Mutagenesis and Deep Sequencing

Rifat

Chen

Kreiswirth

et al. 2021

mBio

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Limited knowledge regarding Mycobacterium abscessus pathogenesis and intrinsic resistance to most classes of antibiotics is a major obstacle to developing more effective strategies to prevent and mitigate disease. Using optimized procedures for Himar1 transposon mutagenesis and deep sequencing, we performed a comprehensive analysis to identify M. abscessus genetic elements essential for in vitro growth and compare them to similar data sets for M. tuberculosis and M. avium subsp. hominissuis .

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Complete Genome Sequence of Mycobacterium chelonae Type Strain CCUG 47445, a Rapidly Growing Species of Nontuberculous Mycobacteria

Cited by 2 publications

References 19 publications

Drug Target Identification and Virtual Screening in Pursuit of Phytochemical Intervention ofMycobacterium chelonae

Drug Target Identification and Virtual Screening in Pursuit of Phytochemical Intervention ofMycobacterium chelonae

Genome-Wide Essentiality Analysis of Mycobacterium abscessus by Saturated Transposon Mutagenesis and Deep Sequencing

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