Complete Genome Sequence of Mycobacterium vaccae Type Strain ATCC 25954

Ho, Yung Shwen; Adroub, Sabir A.; Abadi, Maram; Alwan, Bader Al; Alkhateeb, Reham; Gao, Ge; Ragab, Ala’a; Ali, Shahjahan; Soolingen, Dick van; Bitter, Wilbert; Pain, Arnab; Abdallah, Abdallah

doi:10.1128/jb.01462-12

Cited by 8 publications

(6 citation statements)

References 12 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, these avirulent mycobacterial strains including H37Ra could provide partial cross-protection against M. tuberculosis infection based on shared common antigens. Although no similarity sequence of Ag85A from M. tuberculosis was found in the genome of M. vaccae, which has been newly finished the program of genomic sequencing [12], immunization with heat-killing M. vaccae could result in the generation of CD8 ? T cells which kill macrophages infected with M. tuberculosis [29], which might explain the effective effects against infection with virulent M. tuberculosis.…”

Section: Discussionmentioning

confidence: 99%

Differential Immune Responses and Protective Effects in Avirulent Mycobacterial Strains Vaccinated BALB/c Mice

Liu

Yuan

et al. 2015

Curr Microbiol

View full text Add to dashboard Cite

Screening live mycobacterial vaccine candidates is the important strategy to develop new vaccines against adult tuberculosis (TB). In this study, the immunogenicity and protective efficacy of several avirulent mycobacterial strains including Mycobacterium smegmatis, M. vaccae, M. terrae, M. phlei, M. trivial, and M. tuberculosis H37Ra were compared with M. bovis BCG in BALB/c mice. Our results demonstrated that differential immune responses were induced in different mycobacterial species vaccinated mice. As BCG-vaccinated mice did, M. terrae immunization resulted in Th1-type responses in the lung, as well as splenocytes secreting IFN-γ against a highly conserved mycobacterial antigen Ag85A. M. smegmatis also induced the same splenocytes secreting IFN-γ as BCG and M. terrae did. In addition, M. terrae and M. smegmatis-immunized mice predominantly increased expression of IL-10 and TGF-β in the lung. Most importantly, mice vaccinated with H37Ra and M. vaccae could provide the same protection in the lung against virulent M. tuberculosis challenge as BCG. The result may have important implications in developing adult TB vaccine.

show abstract

Section: Discussionmentioning

confidence: 99%

Differential Immune Responses and Protective Effects in Avirulent Mycobacterial Strains Vaccinated BALB/c Mice

Liu

Yuan

et al. 2015

Curr Microbiol

View full text Add to dashboard Cite

show abstract

“…In order to assess their potential antimycobacterial activity, we evaluated the protein effect on bacterial viability. Mycobacterium vaccae was chosen as a rapidly growing nonvirulent and suitable working species model (61). Although the species infects cattle (62) and is generally considered nonpathogenic to humans, a few cases of cutaneous and pulmonary infection in farm workers have been reported (63).…”

Section: Resultsmentioning

confidence: 99%

Two Human Host Defense Ribonucleases against Mycobacteria, the Eosinophil Cationic Protein (RNase 3) and RNase 7

Pulido

Torrent

Andreu

et al. 2013

Antimicrob Agents Chemother

View full text Add to dashboard Cite

There is an urgent need to develop new agents against mycobacterial infections, such as tuberculosis and other respiratory tract or skin affections. In this study, we have tested two human antimicrobial RNases against mycobacteria. RNase 3, also called the eosinophil cationic protein, and RNase 7 are two small cationic proteins secreted by innate cells during host defense. Both proteins are induced upon infection displaying a wide range of antipathogen activities. In particular, they are released by leukocytes and epithelial cells, contributing to tissue protection. Here, the two RNases have been proven effective against Mycobacterium vaccae at a low micromolar level. High bactericidal activity correlated with their bacterial membrane depolarization and permeabilization activities. Further analysis on both protein-derived peptides identified for RNase 3 an N-terminus fragment that is even more active than the parental protein. Also, a potent bacterial agglutinating activity was unique to RNase 3 and its derived peptide. The particular biophysical properties of the RNase 3 active peptide are envisaged as a suitable reference for the development of novel antimycobacterial drugs. The results support the contribution of secreted RNases to the host immune response against mycobacteria.

show abstract

“…More than a third of the RGM in our study were involved in surgical complications, including M. senegalense, members of the M. smegmatis group, and the above-mentioned unclassified scotochromogens, which underlines the fact that NTM involved in surgical wound infections are not restricted to members of the M. abscessus complex. A remarkable finding is the isolation of M. vanbaalenii, an RGM closely related to M. vaccae, 33 in a patient with a rare recurrent meningeal syndrome. Regarding the involvement of M. peregrinum in human disease, in line with other authors, we were able to reliably assess its aetiological role in our pulmonary case but not in the case of urinary infection.…”

Section: Discussionmentioning

confidence: 99%

Combined approach to the identification of clinically infrequent non-tuberculous mycobacteria in Argentina

Monteserin¹,

Paul²,

López³

et al. 2016

int j tuberc lung dis

View full text Add to dashboard Cite

show abstract

Complete Genome Sequence of Mycobacterium vaccae Type Strain ATCC 25954

Abstract: e Mycobacterium vaccae is a rapidly growing, nontuberculous Mycobacterium species that is generally not considered a human pathogen and is of major pharmaceutical interest as an immunotherapeutic agent. We report here the annotated genome sequence of the M. vaccae type strain, ATCC 25954.

Cited by 8 publications

References 12 publications

Differential Immune Responses and Protective Effects in Avirulent Mycobacterial Strains Vaccinated BALB/c Mice

Differential Immune Responses and Protective Effects in Avirulent Mycobacterial Strains Vaccinated BALB/c Mice

Two Human Host Defense Ribonucleases against Mycobacteria, the Eosinophil Cationic Protein (RNase 3) and RNase 7

Combined approach to the identification of clinically infrequent non-tuberculous mycobacteria in Argentina

Contact Info

Product

Resources

About