Complete Genome Sequence of
            <i>Xanthomonas</i>
            Siphophage Samson

Clark, Sabrina D.; Le, Tram; Moreland, Russell; Liu, Mei; Gonzalez, Carlos F.; Gill, Jason J.; Ramsey, Jolene

doi:10.1128/mra.01097-19

Cited by 2 publications

(2 citation statements)

References 20 publications

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“…no. MZ927746) with 40% query coverage sharing 93.74% to 96.68% identity ( Clark et al, 2019 ; Loh et al, 2021 ). Comparative genomics of NR08 phage with ten Xoophages reported by Kovács et al (2019) (Accession no.…”

Section: Discussionmentioning

confidence: 99%

Isolation of bacteriophages infecting Xanthomonas oryzae pv. oryzae and genomic characterization of novel phage vB_XooS_NR08 for biocontrol of bacterial leaf blight of rice

Jain

Kumar²,

Jain³

et al. 2023

Front. Microbiol.

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Bacterial leaf blight (BLB) disease of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive diseases worldwide in rice-growing regions. The Ineffectiveness of chemicals in disease management has increased the interest in phage therapy. In this study, we isolated 19 bacteriophages, infecting Xoo, from a rice field, which belonged to phage families Siphoviridae, Myoviridae, and Podoviridae on the basis of electron microscopy. Among 19 phages, Phage vB_XooS_NR08, a member of the Siphoviridae family, expressed antibacterial activity against all Xoo strains tested and did not lyse X. campestris and other unrelated bacterial hosts. Phage NR08 showed more than 80% viability at a temperature range of 4°C–40°C, pH range of 5–9, and direct exposure to sunlight for 2 h, whereas UV light and chemical agents were highly detrimental. In a one-step growth curve, NR08 has a 40-min latent period, followed by a 30-min burst period with a burst size of 250 particle/bacterium. The genome of NR08 is double-stranded DNA, linear having a size of 98,812 bp with a G + C content of 52.9%. Annotation of the whole-genome sequence indicated that NR08 encodes 142 putative open reading frames (ORFs), including one ORF for tRNA, namely, trna1-GlnTTG. Comparative genome analysis of NR08 showed that it shares maximum similarity with Pseudomonas phage PaMx42 (40% query coverage, 95.39% identity, and acc. Length 43,225) and Xanthomonas phage Samson (40% query coverage, 96.68% identity, and acc. Length 43,314). The average alignment percentage (AP) of NR08 with other Xoophages was only 0.32 to 1.25 since the genome of NR08 (98.8 kb) is almost double of most of the previously reported Xoophages (43–47 kb), thus indicating NR08 a novel Xoophage. In in vitro bacterial challenge assay, NR08 showed bacteriostasis up to 24 h and a 99.95% reduction in bacterial growth in 48 h. In rice pot efficacy trials, single-dose treatment of NR08 showed a significant reduction in disease up to 90.23% and 79.27% on 7 and 21 dpi, respectively. However, treatment using 2% skim milk-supplemented phage preparation was significantly less effective as compared to the neat phage preparation. In summary, this study characterized a novel Xoophage having the potential as a biocontrol agent in the mitigation of BLB in rice.

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Section: Discussionmentioning

confidence: 99%

Isolation of bacteriophages infecting Xanthomonas oryzae pv. oryzae and genomic characterization of novel phage vB_XooS_NR08 for biocontrol of bacterial leaf blight of rice

Jain

Kumar²,

Jain³

et al. 2023

Front. Microbiol.

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“…punicae Bacterial leaf blight Pomegranate [ 41 ] 1, 20, 22, ΦPS, ΦSD, ΦSL, ΦRS, Φ56, Φ112, Pg60 Sewage, compost, Xp infected soil, seed & dry bean straw X. phaseoli Common blight of beans Beans [ 74 ] Pg176, Pg177, Pg181, Xp infected soil X. phaseoli Common blight of beans Beans [ 75 ] Xanthomonas Siphophage Samson Sewage X. sp. strain ATCC PTA-13101 Bacterial leaf blight Rice [ 76 ] Xanthomonas phage pagan Fresh water X. sp. strain ATCC PTA-13101 Bacterial leaf blight Rice [ 42 ] Xanthomonas phage XacN1 Xci infected soil X. citri Asian citrus canker Orange [ 37 ] BP60C 1–3 , Bp 10 , Bp 20 , Bp 22 Xcj infected soil X. campestris pv.…”

Section: Main Textmentioning

confidence: 99%

Xanthomonas bacteriophages: a review of their biology and biocontrol applications in agriculture

et al. 2021

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Phytopathogenic bacteria are economically important because they affect crop yields and threaten the livelihoods of farmers worldwide. The genus Xanthomonas is particularly significant because it is associated with some plant diseases that cause tremendous loss in yields of globally essential crops. Current management practices are ineffective, unsustainable and harmful to natural ecosystems. Bacteriophage (phage) biocontrol for plant disease management has been of particular interest from the early nineteenth century to date. Xanthomonas phage research for plant disease management continues to demonstrate promising results under laboratory and field conditions. AgriPhage has developed phage products for the control of Xanthomonas campestris pv. vesicatoria and Xanthomonas citri subsp. citri. These are causative agents for tomato, pepper spot and speck disease as well as citrus canker disease.Phage-mediated biocontrol is becoming a viable option because phages occur naturally and are safe for disease control and management. Thorough knowledge of biological characteristics of Xanthomonas phages is vital for developing effective biocontrol products. This review covers Xanthomonas phage research highlighting aspects of their ecology, biology and biocontrol applications.

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Complete Genome Sequence of Xanthomonas Siphophage Samson

Cited by 2 publications

References 20 publications

Isolation of bacteriophages infecting Xanthomonas oryzae pv. oryzae and genomic characterization of novel phage vB_XooS_NR08 for biocontrol of bacterial leaf blight of rice

Isolation of bacteriophages infecting Xanthomonas oryzae pv. oryzae and genomic characterization of novel phage vB_XooS_NR08 for biocontrol of bacterial leaf blight of rice

Xanthomonas bacteriophages: a review of their biology and biocontrol applications in agriculture

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