Complete Genome Sequence of Tepidibacter sp. Strain 8C15b, Isolated from Bank Sediments of Haiphong Bay, Vietnam

Abstract

We report the complete genome sequence of Tepidibacter sp. strain 8C15b, isolated from bank sediments of Haiphong Bay, Vietnam. The genome includes a 3,628,320-bp circular chromosome and a plasmid of 38,213 bp.

Tepidibacter aestuarii sp. nov., isolated from the Bach Dang Estuary, Haiphong, Vietnam

“…ANI and AAI genome sequence comparisons between strains 8C15b T and T. mesophilus B1 T indicated values of only 88.6 and 89.3 % respectively, which is below the level of species differentiation [10]. Moreover, a dDDH value of 38 % was obtained between the two strains.…”

“…Though this strain exhibited maximum similarity to T. mesophilus B1 T in terms of 16S rRNA gene sequences (99.2 % identity), it differed at the whole genome sequence level (89.5 % identity). Plus, an extra plasmid was observed in 8C15b T [10]. Moreover, strain 8C15b T possesses several genes involved in metal resistance and was shown to be tolerant to high nickel concentrations using NiCl 2 .…”

“…Enrichment and isolation of strain 8C15b T were performed by inoculating in a rich medium 200 mg of the first 5 cm sediments of the Bach Dang Estuary, Haiphong, collected in September 2019 and chilled in a cooler box with dry ice for transportation to the laboratory [10]. The rich medium contained: 0.02 % (w/v) KH 2 PO 4 , 0.02 % (w/v) K 2 HPO 4 , 0.005 % (w/v) NH 4 Cl, 2.4 % (w/v) NaCl, 0.6 % (w/v) MgCl 2 ·6H 2 O, 0.4 % (w/v) MgSO 4 ·7H 2 O, 0.1 % (w/v) KCl, 0.1 % CaCl 2 ·2H 2 O, 0.04 % (w/v) cysteine–HCl, 0.1 % (w/v) yeast extract (Difco Laboratories), 0.2 % (w/v) biotrypcase (Difco Laboratories), 0.2 % (w/v) MES (2-( N -morpholino) ethane sulphonic acid), 0.1 % (v/v) Widdel’s trace mineral element solution [11], 0.1 % (v/v) selenite–tungstate solution (see medium DSMZ 385) and 0.0001 % (w/v) resazurin (Sigma-Aldrich).…”

“…The first two strains are both thermophilic micro-organisms: T. thalassicus SC562 T was isolated from hydrothermal vents at 13° N on the East-Pacific Rise at a depth of 2650 m [7]; T. formicigenes DV1184 T was isolated from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge [8]; and T. mesophilus B1 T was isolated from soil polluted by crude oil in the Karamay Oil Field, China, and is a mesophilic micro-organism [9]. In this study, we report the physiological and genetic characterization of strain 8C15b T , isolated about 50 km north of the Red River Delta, in the Bach Dang Estuary, Haiphong, Vietnam [10]. We found this strain to be phylogenetically related to T. mesophilus B1 T .…”

“…mesophilus B1 T was isolated from soil polluted by crude oil in the Karamay Oil Field, China, and is a mesophilic micro-organism [9]. In this study, we report the physiological and genetic characterization of strain 8C15b T , isolated about 50 km north of the Red River Delta, in the Bach Dang Estuary, Haiphong, Vietnam [10]. We found this strain to be phylogenetically related to T.…”