Complete genome sequence of Streptococcus troglodytae TKU31 isolated from the oral cavity of a chimpanzee (Pan troglodytes)

Abstract

Streptococcus troglodytae TKU31 was isolated from the oral cavity of a chimpanzee (Pan troglodytes) and was found to be the most closely related species of the mutans group streptococci to Streptococcus mutans. The complete sequence of TKU31 genome consists of a single circular chromosome that is 2,097,874 base pairs long and has a G + C content of 37.18%. It possesses 2082 coding sequences (CDSs), 65 tRNAs and five rRNA operons (15 rRNAs). Two clustered regularly interspaced short palindromic repeats, six insertion sequences and two predicted prophage elements were identified. The genome of TKU31 harbors some putative virulence associated genes, including gtfB, gtfC and gtfD genes encoding glucosyltransferase and gbpA, gbpB, gbpC and gbpD genes encoding glucan-binding cell wall-anchored protein. The deduced amino acid identity of the rhamnose-glucose polysaccharide F gene (rgpF), which is one of the serotype determinants, is 91% identical with that of S. mutans LJ23 (serotype k) strain. However, two other virulence-associated genes cnm and cbm, which encode the collagen-binding proteins, were not found in the TKU31 genome. The complete genome sequence of S. troglodytae TKU31 has been deposited at DDBJ/European Nucleotide Archive/GenBank under the accession no. AP014612.

An anaerobic bacterium host system for heterologous expression of natural product biosynthetic gene clusters

“…Of the five remaining BGCs, all of which encode unknown products, BGC1 stands out for being confined to Streptococcus species found in the mouths of primates, including humans. Database searching showed that gene clusters having the same organization as BGC1 were found in 25 S. mutans and two other Streptococcus strains, S. troglodytae TKU31 39 , and S. macacae NCTC 11558 40 (Supplementary Fig. 4).…”

“…16a). The proposed biosynthetic pathway of reutericyclin 39 involves addition of the C7-acetyl group as the final step, with thioesterase-catalyzed lactam bond formation to afford the pyrrolidine ring (Supplementary Fig. 16b).…”

Propofol infusions using a human target controlled infusion (TCI) pump in chimpanzees (Pan troglodytes)

“…There are collaborative efforts between human medical professionals (doctors and dentists) and veterinarians including the great ape heart project 30 and chimpanzee dental project (collaboration between KUPRI and Tsurumi University, e.g. 31 ). The collaboration and "One Health approach" 32 are particularly fruitful in conservation of endangered great ape species in captivity.…”

Endodontic Treatment of a Maxillary Incisor Tooth in a Chimpanzee (Pan troglodytes)

“…Researchers and veterinarians at the Primate Research Institute of Kyoto University and human dental professionals at the Tsurumi University School of Dental Medicine have collaborated in the "Chimpanzee Dental Project" to improve oral health in chimpanzees and they found new strains of oral bacteria closely related to the mutans streptococci group. 4 During this project, human dental professionals had the opportunity to treat apical periodontitis in a female chimpanzee (Figure 1A). The endodontic treatment used in humans was adapted for this chimpanzee.…”