Complete genome sequence of
            <i>Pseudomonas aeruginosa</i>
            phage Knedl

Maffei, Enea; Manner, Christina; Jenal, Urs; Harms, Alexander

doi:10.1128/mra.01174-23

Microbiol Resour Announc

2024

DOI: 10.1128/mra.01174-23

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Complete genome sequence of Pseudomonas aeruginosa phage Knedl

Enea Maffei,

Christina Manner,

Urs Jenal

et al.

Abstract: Bacteriophage Knedl is the first reported Pseudomonas aeruginosa phage that targets the Psl exopolysaccharide as receptor. Here, we report the genome of Knedl, demonstrating that it belongs to the genus Iggyvirus of the Queuovirinae subfamily. Future studies on the infection mechanism of Knedl could inform phage-based approaches to eradicate biofilms.

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A biofilm-tropicPseudomonas aeruginosabacteriophage uses the exopolysaccharide Psl as receptor

Walton,

Abbondante,

Marshall

et al. 2024

Preprint

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Bacteria in nature can exist in multicellular communities called biofilms. Biofilms also form in the course of many infections. Pseudomonas aeruginosa infections frequently involve biofilms, which contribute materially to the difficulty to treat these infections with antibiotic therapy. Many biofilm-related characteristics are controlled by the second messenger, cyclic-di-GMP, which is upregulated on surface contact. Among these factors is the exopolysaccharide Psl, which is a critically important component of the biofilm matrix. Here we describe the discovery of a bacteriophage, which we have called Clew-1, that directly binds to and uses Psl as a receptor for attaching to P. aeruginosa. While this phage does not efficiently infect logarithmically growing bacteria, it can disrupt P. aeruginosa biofilms and replicate on biofilm bacteria. We further demonstrate that the bacteriophage can reduce the bacterial burden in a mouse model of P. aeruginosa keratitis, which is characterized by the formation of a biofilm on the cornea. Due to its reliance on Psl for infection, Clew-1 does not actually form plaques on wild-type bacteria under standard in vitro conditions. This argues that our standard isolation procedures likely exclude bacteriophage that are adapted to using biofilm markers for infection. Importantly, the manner in which we isolated Clew-1 can be easily extended to other strains of P. aeruginosa, which will fuel the discovery of other biofilm-tropic bacteriophage and expand their therapeutic use.

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A biofilm-tropicPseudomonas aeruginosabacteriophage uses the exopolysaccharide Psl as receptor

Walton,

Abbondante,

Marshall

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Complete genome sequence of Pseudomonas aeruginosa phage Knedl

Cited by 1 publication

References 9 publications

A biofilm-tropicPseudomonas aeruginosabacteriophage uses the exopolysaccharide Psl as receptor

A biofilm-tropicPseudomonas aeruginosabacteriophage uses the exopolysaccharide Psl as receptor

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