Complete Genome Sequence of
            <i>Pseudomonas</i>
            sp. Strain NC02, Isolated from Soil

Cerra, Joseph; Donohue, Hailey; Kral, Alexander; Oser, Molly; Rostkowski, Laura; Zappia, Luke; Williams, Laura E.

doi:10.1128/genomea.00033-18

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…To enrich for predatory bacteria in the soil sample supernatant, we used Pseudomonas sp. NC02 as prey, because it was also isolated from a soil environment, and its genome has been sequenced [35]. We cultured Pseudomonas sp.…”

Section: Isolation and Identification Of Bdellovibrio Sp Nc01 From Bmentioning

confidence: 99%

Variation in genome content and predatory phenotypes between Bdellovibrio sp. NC01 isolated from soil and B. bacteriovorus type strain HD100

et al. 2019

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Defining phenotypic and associated genotypic variation among Bdellovibrio may further our understanding of how this genus attacks and kills different Gram-negative bacteria. We isolated Bdellovibrio sp. NC01 from soil. Analysis of 16S rRNA gene sequences and average amino acid identity showed that NC01 belongs to a different species than the type species bacteriovorus. By clustering amino acid sequences from completely sequenced Bdellovibrio and comparing the resulting orthologue groups to a previously published analysis, we defined a 'core genome' of 778 protein-coding genes and identified four proteincoding genes that appeared to be missing only in NC01. To determine how horizontal gene transfer (HGT) may have impacted NC01 genome evolution, we performed genome-wide comparisons of Bdellovibrio nucleotide sequences, which indicated that eight NC01 genomic regions were likely acquired by HGT. To investigate how genome variation may impact predation, we compared protein-coding gene content between NC01 and the B. bacteriovorus type strain HD100, focusing on genes implicated as important in successful killing of prey. Of these, NC01 is missing ten genes that may play roles in lytic activity during predation. Compared to HD100, NC01 kills fewer tested prey strains and kills Escherichia coli ML35 less efficiently. NC01 causes a smaller log reduction in ML35, after which the prey population recovers and the NC01 population decreases. In addition, NC01 forms turbid plaques on lawns of E. coli ML35, in contrast to clear plaques formed by HD100. Linking phenotypic variation in interactions between Bdellovibrio and Gram-negative bacteria with underlying Bdellovibrio genome variation is valuable for understanding the ecological significance of predatory bacteria and evaluating their effectiveness in clinical applications.

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Section: Isolation and Identification Of Bdellovibrio Sp Nc01 From Bmentioning

confidence: 99%

Variation in genome content and predatory phenotypes between Bdellovibrio sp. NC01 isolated from soil and B. bacteriovorus type strain HD100

et al. 2019

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“…NC02 as prey. Not only was this strain of Pseudomonas originally isolated from soil ( 20 ), but it was also used as prey during the initial stages of Bdellovibrio sp. NC01 isolation, as detailed in Materials and Methods.…”

Section: Resultsmentioning

confidence: 99%

Parallel Evolution in Predatory Bdellovibrio sp. NC01 during Long-Term Coculture with a Single Prey Strain

Mulvey

Brosnan

Galvin

et al. 2023

Appl Environ Microbiol

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“…Pseudomonas sp. NC02 is a recently isolated soil bacterium about which not much is known [ 46 ]. P. edaphica is another newly identified fluorescent Pseudomonas that was isolated from rhizosphere soil, and is closely related to P. brenneri [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Pseudomonas from Agaricus bisporus caps reveal novel blotch pathogens in Western Europe

et al. 2020

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Background: Bacterial blotch is a group of economically important diseases affecting the cultivation of common button mushroom, Agaricus bisporus. Despite being studied for more than a century, the identity and nomenclature of blotch-causing Pseudomonas species is still unclear. This study aims to molecularly characterize the phylogenetic and phenotypic diversity of blotch pathogens in Western Europe. Methods: In this study, blotched mushrooms were sampled from farms across the Netherlands, United Kingdom and Belgium. Bacteria were isolated from symptomatic cap tissue and tested in pathogenicity assays on fresh caps and in pots. Whole genome sequences of pathogenic and non-pathogenic isolates were used to establish phylogeny via multi-locus sequence alignment (MLSA), average nucleotide identity (ANI) and in-silico DNA:DNA hybridization (DDH) analyses. Results: The known pathogens "Pseudomonas gingeri", P. tolaasii, "P. reactans" and P. costantinii were recovered from blotched mushroom caps. Seven novel pathogens were also identified, namely, P. yamanorum, P. edaphica, P. salomonii and strains that clustered with Pseudomonas sp. NC02 in one genomic species, and three nonpseudomonads, i.e. Serratia liquefaciens, S. proteamaculans and a Pantoea sp. Insights on the pathogenicity and symptom severity of these blotch pathogens were also generated. Conclusion: A detailed overview of genetic and regional diversity and the virulence of blotch pathogens in Western Europe, was obtained via the phylogenetic and phenotypic analyses. This information has implications in the study of symptomatic disease expression, development of diagnostic tools and design of localized strategies for disease management.

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Complete Genome Sequence of Pseudomonas sp. Strain NC02, Isolated from Soil

Cited by 4 publications

References 11 publications

Variation in genome content and predatory phenotypes between Bdellovibrio sp. NC01 isolated from soil and B. bacteriovorus type strain HD100

Variation in genome content and predatory phenotypes between Bdellovibrio sp. NC01 isolated from soil and B. bacteriovorus type strain HD100

Parallel Evolution in Predatory Bdellovibrio sp. NC01 during Long-Term Coculture with a Single Prey Strain

Molecular characterization of Pseudomonas from Agaricus bisporus caps reveal novel blotch pathogens in Western Europe

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