Complete Genome Sequence of
            <i>Porphyrobacter</i>
            sp. Strain CACIAM 03H1, a Proteobacterium Obtained from a Nonaxenic Culture of Microcystis aeruginosa

Castro, Wendel de Oliveira; Lima, Alex Ranieri Jerônimo; Moraes, Pablo Henrique Gonçalves; Siqueira, Andrei Santos; Aguiar, Délia Cristina Figueira; Baraúna, Anna Rafaella Ferreira; Martins, Luisa Carí­cio; Fuzii, Hellen Thaís; Couto, Danielle Costa Carrara; Francês, Regiane Silva Kawasaki; Lima, Clayton Pereira Silva de; Júnior, João Lídio da Silva Gonçalves Vianez; Nunes, Márcio Roberto Teixeira; Dall'Agnol, Leonardo Teixeira; Gonçalves, Evonnildo Costa

doi:10.1128/genomea.01069-17

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…ULC335 together with homologs for PpsR, AppA, and FnrL, known to integrate phototrophy with metabolism[65, 66]. Because Porphyrobacter strains are frequently associated with filamentous or colonial Cyanobacteria [67–69] their physiology could also indirectly follow the light and circadian clock-driven life cycle of their host like it has been suggested for other Cyanobacteria-associated heterotrophs [70]. This makes members of the Porphyrobacter genus excellent candidates for the study of bacterial rhythmic behaviors and interactions.…”

Section: Discussionmentioning

confidence: 99%

“…Because of the phylogenetic prevalence of AAnP in the clade, these organisms are expected to have evolved ways to tune their physiology to nutrient and light fluctuations, as evidenced by the high numbers of blue-light sensing proteins encoded in the genome of Porphyrobacter sp. ULC335 together with homologs for PpsR, AppA, and FnrL, known to integrate phototrophy with metabolism[65,66].Because Porphyrobacter strains are frequently associated with filamentous or colonial Cyanobacteria[67][68][69] their physiology could also indirectly follow the light and circadian clock-driven life cycle of their host like it has been suggested for other Cyanobacteria-associated heterotrophs[70]. This makes members of the Porphyrobacter genus excellent candidates for the study of bacterial rhythmic behaviors and interactions.The conspicuous light-driven oscillations in optical density we detected in batch cultures of Porphyrobacter sp.…”

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confidence: 96%

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Diurnal cycles drive rhythmic physiology and promote survival in facultative phototrophic bacteria

Tinguely,

Paulméry,

Terrettaz

et al. 2023

Preprint

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SummaryBacteria have evolved many strategies to spare energy when nutrients become scarce. One widespread such strategy is facultative phototrophy, which helps heterotrophs supplement their energy supply using light. Our knowledge on the impact that such behaviors have on bacterial fitness and physiology is, however, still limited. Here, we study how a representative of the genusPorphyrobacter,in which aerobic anoxygenic phototrophy is ancestral, responds to different light regimes under nutrient limitation. We show that bacterial survival in stationary phase relies on functional reaction centers and varies depending on the light regime. Under dark-light alternance, our bacterial model presents a diphasic life history dependent on phototrophy: during dark phases, the cells inhibit DNA replication and part of the population lyses and releases nutrients, while subsequent light phases allow for the recovery and renewed growth of the surviving cells. We correlate these cyclic variations with a pervasive pattern of rhythmic transcription which reflects global changes in diurnal metabolic activity. Finally, we demonstrate that, compared to either a phototrophy null mutant or a bacteriochlorophyllaoverproducer, the wild type strain is better adapted to natural environments, where regular dark-light cycles are interspersed with additional accidental dark episodes. Overall, our results highlight the importance of light-induced biological rhythms in a new model of aerobic anoxygenic phototroph representative of an ecologically important group of environmental bacteria.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 96%

Diurnal cycles drive rhythmic physiology and promote survival in facultative phototrophic bacteria

Tinguely,

Paulméry,

Terrettaz

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Diurnal cycles drive rhythmic physiology and promote survival in facultative phototrophic bacteria

Tinguely,

Paulméry,

Terrettaz

et al. 2023

ISME Communications

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Bacteria have evolved many strategies to spare energy when nutrients become scarce. One widespread such strategy is facultative phototrophy, which helps heterotrophs supplement their energy supply using light. Our knowledge of the impact that such behaviors have on bacterial fitness and physiology is, however, still limited. Here, we study how a representative of the genus Porphyrobacter, in which aerobic anoxygenic phototrophy is ancestral, responds to different light regimes under nutrient limitation. We show that bacterial survival in stationary phase relies on functional reaction centers and varies depending on the light regime. Under dark-light alternance, our bacterial model presents a diphasic life history dependent on phototrophy: during dark phases, the cells inhibit DNA replication and part of the population lyses and releases nutrients, while subsequent light phases allow for the recovery and renewed growth of the surviving cells. We correlate these cyclic variations with a pervasive pattern of rhythmic transcription which reflects global changes in diurnal metabolic activity. Finally, we demonstrate that, compared to either a phototrophy mutant or a bacteriochlorophyll a overproducer, the wild type strain is better adapted to natural environments, where regular dark-light cycles are interspersed with additional accidental dark episodes. Overall, our results highlight the importance of light-induced biological rhythms in a new model of aerobic anoxygenic phototroph representative of an ecologically important group of environmental bacteria.

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Genome sequence, metabolic properties and cyanobacterial attachment of Porphyrobacter sp. HT-58-2 isolated from a filamentous cyanobacterium–microbial consortium

et al. 2018

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Tolyporphins are structurally diverse tetrapyrrole macrocycles produced by the cyanobacterial culture HT-58-2. Although tolyporphins were discovered over 25 years ago, little was known about the microbiology of the culture. The studies reported herein expand the description of the community of predominantly alphaproteobacteria associated with the filamentous HT-58-2 cyanobacterium and isolate a dominant bacterium, Porphyrobacter sp. HT-58-2, for which the complete genome is established and growth properties are examined. Fluorescence in situ hybridization (FISH) analysis of the cyanobacterium-microbial community with a probe targeting the 16S rRNA of Porphyrobacter sp. HT-58-2 showed fluorescence emanating from the cyanobacterial sheath. Although genes for the biosynthesis of bacteriochlorophyll a (BChl a) are present in the Porphyrobacter sp. HT-58-2 genome, the pigment was not detected under the conditions examined, implying the absence of phototrophic growth. Comparative analysis of four Porphyrobacter spp. genomes from worldwide collection sites showed significant collinear gene blocks, with two inversions and three deletion regions. Taken together, the results enrich our understanding of the HT-58-2 cyanobacterium-microbial culture.

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Complete Genome Sequence of Porphyrobacter sp. Strain CACIAM 03H1, a Proteobacterium Obtained from a Nonaxenic Culture of Microcystis aeruginosa

Abstract: Here, we present a complete genome sequence and annotation of Porphyrobacter sp. strain CACIAM 03H1, which was obtained from a nonaxenic culture of Microcystis aeruginosa, a cyanobacterium isolated from an Amazonian freshwater environment.

Cited by 3 publications

References 7 publications

Diurnal cycles drive rhythmic physiology and promote survival in facultative phototrophic bacteria

Diurnal cycles drive rhythmic physiology and promote survival in facultative phototrophic bacteria

Diurnal cycles drive rhythmic physiology and promote survival in facultative phototrophic bacteria

Genome sequence, metabolic properties and cyanobacterial attachment of Porphyrobacter sp. HT-58-2 isolated from a filamentous cyanobacterium–microbial consortium

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