Complete genome sequence of Photobacterium ganghwense C2.2: A new polyhydroxyalkanoate production candidate

Abstract

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Scalable and Sustainable Processing of Intracellular Polyhydroxyalkanoates with Biobased Solvents

“…Lyophilized cells (9.8 wt %) in Cyrene showed a broad endothermic peak with maximum at 113 °C, which is close to the extraction temperature in the glass vial experiments. Broadening of the peak is most likely associated with proteins in the cells. , Heating of a polymer–solvent mixture produced two endothermic peaks in the thermogram, where the first peak at 75 °C is interpreted as swelling of the polymer, T swell , followed by the main dissolution temperature peak, T dis , at 84 °C. The lower dissolution temperature of the polymer reference preparation compared to that of the intact “pristine” polymer in the dry whole cells is indicative of a lower fraction of crystalline phases in the dissolved and precipitated polymer.…”

“…Photobacterium ganghwense strain C2.2 was repeatedly streaked on solid medium MP at 20 °C, in order to obtain isolated colonies. A single colony was inoculated in 250 mL of liquid MP and cultivated at 20 °C with stirring at 150 rpm, for 20 h, until the culture reached an OD 600 of 5.5.…”

Comparative Genomic Analyses of the Genus Photobacterium Illuminate Biosynthetic Gene Clusters Associated with Antagonism

“…The following supporting information can be downloaded at: . References [ 4 , 5 , 7 , 17 , 29 , 30 , 34 , 35 , 36 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 ] are cited in the supplementary materials.…”