Complete Genome Sequence of
            <i>Neisseria gonorrhoeae</i>
            NCCP11945

Chung, Gyung Tae; Yoo, Jeong Sik; Oh, Hee-Bok; Lee, Yeong Seon; Ho, Sun; Kim, Sang Jun; Yoo, Cheon-Kwon

doi:10.1128/jb.00566-08

Cited by 64 publications

(77 citation statements)

References 9 publications

(1 reference statement)

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“…4B); this region is flanked by regions of high sequence identity (88.5% to 94.2%). Similar results were obtained in a BLASTn search of the nucleotide database that returned partial alignments among seven meningococcal genomes (strain 8013 [37], ␣14 [41], Z2491 [35], FAM18 [3], 053442 [36], ␣710 [22], and MC58 [46]) and two gonococcal genomes (strains NCCP11945 [8] Analysis of fH binding to strains not expressing fHbp. fH bound to the surface of the meningococcus retains its activity as a cofactor for fI-mediated cleavage of C3b and enhances survival of N. meningitidis in the presence of the human complement system (38).…”

Section: Resultssupporting

confidence: 53%

Characterization of Neisseria meningitidis Isolates That Do Not Express the Virulence Factor and Vaccine Antigen Factor H Binding Protein

Lucidarme

Tan

Exley

et al. 2011

Clin Vaccine Immunol

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Neisseria meningitidis remains a leading cause of bacterial sepsis and meningitis. Complement is a key component of natural immunity against this important human pathogen, which has evolved multiple mechanisms to evade complement-mediated lysis. One approach adopted by the meningococcus is to recruit a human negative regulator of the complement system, factor H (fH), to its surface via a lipoprotein, factor H binding protein (fHbp). Additionally, fHbp is a key antigen in vaccines currently being evaluated in clinical trials. Here we characterize strains of N. meningitidis from several distinct clonal complexes which do not express fHbp; all strains were recovered from patients with disseminated meningococcal disease. We demonstrate that these strains have either a frameshift mutation in the fHbp open reading frame or have entirely lost fHbp and some flanking sequences. No fH binding was detected to other ligands among the fHbp-negative strains. The implications of these findings for meningococcal pathogenesis and prevention are discussed.

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Section: Resultssupporting

confidence: 53%

Characterization of Neisseria meningitidis Isolates That Do Not Express the Virulence Factor and Vaccine Antigen Factor H Binding Protein

Lucidarme

Tan

Exley

et al. 2011

Clin Vaccine Immunol

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“…txf was detected as a sequence inserted into ST1407 contig 061 and is referred to as GR8 in Tables 1 and 2. This inserted sequence deleted 1,106 bp of sequence from the reference NCCP11945 genome (9). Similar to the opa query, this inserted sequence did not map to any region in the NCCP11945 (9) or FA1090 (12) genome.…”

Section: Resultsmentioning

confidence: 93%

“…Using the previously reported NCCP11945 gonococcal genome (9) as a scaffold, we further assembled these 573 contigs into 91 continuous contigs with an average size of 22,500 bp. These 91 larger contigs covered 2,029,064 bp (91.1%) of the NCCP11945 reference genome (9). The vast majority of the gaps between these contigs fell into homopolymeric regions of the N. gonorrhoeae genome.…”

Section: Bacterial Collection (California)mentioning

confidence: 99%

Genome Sequencing of a Neisseria gonorrhoeae Isolate of a Successful International Clone with Decreased Susceptibility and Resistance to Extended-Spectrum Cephalosporins

Hess

Golparian

et al. 2012

Antimicrob Agents Chemother

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eThe recent emergence of Neisseria gonorrhoeae strains with decreased susceptibility to extended-spectrum cephalosporins is a major concern globally. We sequenced the genome of an N. gonorrhoeae multiantigen sequence typing (NG-MAST) ST1407 isolate (SM-3) with decreased susceptibility and resistance to oral extended-spectrum cephalosporins. The isolate was cultured in 2008 in San Francisco, CA, and possessed mosaic penA allele XXXIV, which is associated with an international clone that possesses decreased susceptibility as well as resistance to oral extended-spectrum cephalosporins globally. The genome sequence of strain NCCP11945 was used as a scaffold, and our assembly resulted in 91 contigs covering 2,029,064 bp (91%; >150؋ coverage) of the genome. Numerous instances of suspected horizontal genetic transfer events with other Neisseria species were identified, and two genes, opa and txf, acquired from nongonococcal Neisseria species, were identified. Strains possessing mosaic penA alleles (n ‫؍‬ 108) and nonmosaic penA alleles (n ‫؍‬ 169) from the United States and Europe (15 countries), cultured in 2002 to 2009, were screened for the presence of these genes. The opa gene was detected in most (82%) penA mosaic-containing isolates (mainly from 2007 to 2009) but not in any penA nonmosaic isolates. The txf gene was found in all strains containing opa but also in several (18%) penA nonmosaic strains. Using opa and txf as genetic markers, we identified a strain that possesses mosaic penA allele XXXIV, but the majority of its genome is not genetically related to strain SM-3. This implies that penA mosaic allele XXXIV was transferred horizontally. Such isolates also possessed decreased susceptibility and resistance to oral extended-spectrum cephalosporins. These findings support that genetic screening for particular penA mosaic alleles can be a valuable method for tracking strains with decreased susceptibility as well as resistance to oral extended-spectrum cephalosporins worldwide and that screening using only NG-MAST may not be sufficient.

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“…Despite a high respiratory capacity that is comparable to that of aerobic bacteria, gonococcal physiology is typical of a microaerophile. It has only a single cytochrome oxidase, cytochrome cbb 3 , which in other bacteria has a very high affinity for oxygen (1)(2)(3)(4)(5). We previously suggested that its high respiratory capacity enables gonococci to minimize oxidative damage from reactive oxygen species generated both from its own respiratory metabolism and by other bacteria that share its environment (6).…”

mentioning

confidence: 99%

A Critical Role for the cccA Gene Product, Cytochrome c ₂ , in Diverting Electrons from Aerobic Respiration to Denitrification in Neisseria gonorrhoeae

2013

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Neisseria gonorrhoeae is a microaerophile that, when oxygen availability is limited, supplements aerobic respiration with a truncated denitrification pathway, nitrite reduction to nitrous oxide. We demonstrate that the cccA gene of Neisseria gonorrhoeae strain F62 (accession number NG0292) is expressed, but the product, cytochrome c 2 , accumulates to only low levels. Nevertheless, a cccA mutant reduced nitrite at about half the rate of the parent strain. We previously reported that cytochromes c 4 and c 5 transfer electrons to cytochrome oxidase cbb 3 by two independent pathways and that the CcoP subunit of cytochrome oxidase cbb 3 transfers electrons to nitrite. We show that mutants defective in either cytochrome c 4 or c 5 also reduce nitrite more slowly than the parent. By combining mutations in cccA (⌬c 2 ), cycA (⌬c 4 ), cycB (⌬c 5 ), and ccoP (ccoP-C368A), we demonstrate that cytochrome c 2 is required for electron transfer from cytochrome c 4 via the third heme group of CcoP to the nitrite reductase, AniA, and that cytochrome c 5 transfers electrons to nitrite reductase by an independent pathway. We propose that cytochrome c 2 forms a complex with cytochrome oxidase. If so, the redox state of cytochrome c 2 might regulate electron transfer to nitrite or oxygen. However, our data are more consistent with a mechanism in which cytochrome c 2 and the CcoQ subunit of cytochrome oxidase form alternative complexes that preferentially catalyze nitrite and oxygen reduction, respectively. Comparison with the much simpler electron transfer pathway for nitrite reduction in the meningococcus provides fascinating insights into niche adaptation within the pathogenic neisseriae.

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Complete Genome Sequence of Neisseria gonorrhoeae NCCP11945

Cited by 64 publications

References 9 publications

Characterization of Neisseria meningitidis Isolates That Do Not Express the Virulence Factor and Vaccine Antigen Factor H Binding Protein

Characterization of Neisseria meningitidis Isolates That Do Not Express the Virulence Factor and Vaccine Antigen Factor H Binding Protein

Genome Sequencing of a Neisseria gonorrhoeae Isolate of a Successful International Clone with Decreased Susceptibility and Resistance to Extended-Spectrum Cephalosporins

A Critical Role for the cccA Gene Product, Cytochrome c ₂ , in Diverting Electrons from Aerobic Respiration to Denitrification in Neisseria gonorrhoeae

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Complete Genome Sequence of Neisseria gonorrhoeae NCCP11945

Cited by 64 publications

References 9 publications

Characterization of Neisseria meningitidis Isolates That Do Not Express the Virulence Factor and Vaccine Antigen Factor H Binding Protein

Characterization of Neisseria meningitidis Isolates That Do Not Express the Virulence Factor and Vaccine Antigen Factor H Binding Protein

Genome Sequencing of a Neisseria gonorrhoeae Isolate of a Successful International Clone with Decreased Susceptibility and Resistance to Extended-Spectrum Cephalosporins

A Critical Role for the cccA Gene Product, Cytochrome c 2 , in Diverting Electrons from Aerobic Respiration to Denitrification in Neisseria gonorrhoeae

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A Critical Role for the cccA Gene Product, Cytochrome c ₂ , in Diverting Electrons from Aerobic Respiration to Denitrification in Neisseria gonorrhoeae