Complete Genome Sequence of
            <i>Marinobacter</i>
            sp. CP1, Isolated from a Self-Regenerating Biocathode Biofilm

Zheng, Wei; Eddie, Brian J.; Malanoski, Anthony P.; Hervey, W. Judson; Lin, Baochuan; Glaven, Sarah M.

doi:10.1128/genomea.01103-15

Cited by 11 publications

(10 citation statements)

References 16 publications

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“…Isolation of Marinobacter sp. strain CP1 from an autotrophic biocathode community has been previously described (Wang et al, 2015b), and a closed genome for this strain has been published (Wang et al, 2015a). Phylogenetic trees were generated based on 16S and 23S rRNA gene sequences of Marinobacter spp.…”

Section: Resultsmentioning

confidence: 99%

Development of a Genetic System for Marinobacter atlanticus CP1 (sp. nov.), a Wax Ester Producing Strain Isolated From an Autotrophic Biocathode

et al. 2018

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Here, we report on the development of a genetic system for Marinobacter sp. strain CP1, previously isolated from the Biocathode MCL community and shown to oxidize iron and grow as a cathodic biofilm. Sequence analysis of the small and large subunits of the 16S rRNA gene of CP1, as well as comparison of select conserved proteins, indicate that it is most closely related to Marinobacter adhaerens HP15 and Marinobacter sp. ES.042. In silico DNA–DNA hybridization using the genome-to-genome distance calculator (GGDC) predicts CP1 to be a new species of Marinobacter described here as Marinobacter atlanticus. CP1 is competent for transformation with plasmid DNA using conjugation with Escherichia coli donor strain WM3064 and constitutive expression of green fluorescent protein (GFP) is stable in the absence of antibiotic selection. Targeted double deletion mutagenesis of homologs for the M. aquaeoli fatty acyl-CoA reductase (acrB) and fatty aldehyde reductase (farA) genes resulted in a loss of production of wax esters; however, single deletion mutants for either gene resulted in an increase in total wax esters recovered. Genetic tools presented here for CP1 will enable further exploration of wax ester synthesis for biotechnological applications, as well as furthering our efforts to understand the role of CP1 within the Biocathode MCL community.

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Section: Resultsmentioning

confidence: 99%

Development of a Genetic System for Marinobacter atlanticus CP1 (sp. nov.), a Wax Ester Producing Strain Isolated From an Autotrophic Biocathode

et al. 2018

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“…were assembled to completion from metagenomic DNA and Marinobacter sp. CP1 (Wang et al ., ) and Labrenzia sp. CP4 (Wang et al ., ) were assembled to completion using single‐molecule real‐time (SMRT) sequencing reads.…”

Section: Resultsmentioning

confidence: 97%

“…Ray settings for k ‐mer length and node coverage of 8 were selected based on previous Ray assemblies of Illumina de novo sequencing of Marinobacter sp. strain CP1 (Wang et al ., ) and Labrenzia sp. strain CP4 (Wang et al ., ) as described in the Supplemental Materials and Methods.…”

Section: Methodsmentioning

confidence: 97%

Relative abundance of ‘Candidatus Tenderia electrophaga’ is linked to cathodic current in an aerobic biocathode community

Malanoski

Lin

Eddie

et al. 2017

Microbial Biotechnology

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SummaryBiocathode microbial communities are proposed to catalyse a range of useful reactions. Unlike bioanodes, model biocathode organisms have not yet been successfully cultivated in isolation highlighting the need for culture‐independent approaches to characterization. Biocathode MCL (Marinobacter, Chromatiaceae, Labrenzia) is a microbial community proposed to couple CO 2 fixation to extracellular electron transfer and O2 reduction. Previous metagenomic analysis of a single MCL bioelectrochemical system (BES) resulted in resolution of 16 bin genomes. To further resolve bin genomes and compare community composition across replicate MCL BES, we performed shotgun metagenomic and 16S rRNA gene (16S) sequencing at steady‐state current. Clustering pooled reads from replicate BES increased the number of resolved bin genomes to 20, over half of which were > 90% complete. Direct comparison of unassembled metagenomic reads and 16S operational taxonomic units (OTUs) predicted higher community diversity than the assembled/clustered metagenome and the predicted relative abundances did not match. However, when 16S OTUs were mapped to bin genomes and genome abundance was scaled by 16S gene copy number, estimated relative abundance was more similar to metagenomic analysis. The relative abundance of the bin genome representing ‘Ca. Tenderia electrophaga’ was correlated with increasing current, further supporting the hypothesis that this organism is the electroautotroph.

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“…are described as microbial opportunitrophs for their ability to utilize a wide range of carbon substrates in the environment and outcompete other bacteria [ 1 ]. As part of their metabolic repertoire, many species have been found to use metals (e.g., iron, arsenic, and manganese) as electron donors [ [2] , [3] , [4] ] or acceptors [ [5] , [6] , [7] , [8] ] during both microaerobic and anaerobic growth. Such observations have led to speculation that Marinobacter spp.…”

Section: Introductionmentioning

confidence: 99%

“…Although the role of M. atlanticus in the Biocathode MCL community is unknown, in isolation it can produce or consume small amounts of current when grown as an electrode biofilm. Like other Marinobacter species, M. atlanticus can oxidize or reduce iron when an organic carbon source is also provided, but does not appear to conserve energy for growth and cannot grow autotrophically [ 6 , 16 ]. M. atlanticus is a “weak electricigen” [ 17 ]; when grown on an electrode at potentials that promote electron uptake, current is at least one order of magnitude lower than the Biocathode MCL community [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Marinobacter atlanticus electrode biofilms differentially regulate gene expression depending on electrode potential and lifestyle

Eddie

Malanoski

Onderko

et al. 2021

Biofilm

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Marinobacter spp. are opportunitrophs with a broad metabolic range including interactions with metals and electrodes. Marinobacter atlanticus strain CP1 was previously isolated from a cathode biofilm microbial community enriched from a sediment microbial fuel cell. Like other Marinobacter spp., M. atlanticus generates small amounts of electrical current when grown as a biofilm on an electrode, which is enhanced by the addition of redox mediators. However, the molecular mechanism resulting in extracellular electron transfer is unknown. Here, RNA-sequencing was used to determine changes in gene expression in electrode-attached and planktonic cells of M. atlanticus when grown at electrode potentials that enable current production (310 and 510 mV vs. SHE) compared to a potential that enables electron uptake (160 mV). Cells grown at current-producing potentials had increased expression of genes for molybdate transport, regardless of planktonic or attached lifestyle. Electrode-attached cells at current-producing potentials showed increased expression of the major export protein for the type VI secretion system. Growth at 160 mV resulted in an increase in expression of genes related to stress response and DNA repair including both RecBCD and the LexA/RecA regulatory network, as well as genes for copper homeostasis. Changes in expression of proteins with PEP C-terminal extracellular export motifs suggests that M. atlanticus is remodeling the biofilm matrix in response to electrode potential. These results improve our understanding of the physiological adaptations required for M. atlanticus growth on electrodes, and suggest a role for metal acquisition, either as a requirement for metal cofactors of redox proteins or as a possible electron shuttling mechanism.

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Complete Genome Sequence of Marinobacter sp. CP1, Isolated from a Self-Regenerating Biocathode Biofilm

Cited by 11 publications

References 16 publications

Development of a Genetic System for Marinobacter atlanticus CP1 (sp. nov.), a Wax Ester Producing Strain Isolated From an Autotrophic Biocathode

Development of a Genetic System for Marinobacter atlanticus CP1 (sp. nov.), a Wax Ester Producing Strain Isolated From an Autotrophic Biocathode

Relative abundance of ‘Candidatus Tenderia electrophaga’ is linked to cathodic current in an aerobic biocathode community

Marinobacter atlanticus electrode biofilms differentially regulate gene expression depending on electrode potential and lifestyle

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