Complete Genome Sequence of
            <i>Lymantria dispar multiple nucleopolyhedrovirus</i>
            Isolated in Southwestern Poland

Krejmer-Rabalska, Martyna; Rąbalski, Łukasz; Skrzecz, Iwona; Szewczyk, Bogusław

doi:10.1128/genomea.01422-16

Cited by 4 publications

(1 citation statement)

References 3 publications

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“…The vef -1 gene is also absent in LdMPNV-3041 (Japan) [ 22 ], which is close to our isolate LdMNPV-J2 (Japan) in the phylogenetic analysis of Harrison et al [ 25 ]. In addition, the isolates LdMNPV-RR01 (Poland) [ 42 ], LdMNPV-Nsk-07 (Russia) (MK411292), and LdMNPV-27/0 (Russia) [ 43 ] also lack a vef -1 gene. In the NCBI database, the complete genomes of the other isolates, LdMNPV and LdMNPV-J2, were examined, and it was found that the mucin-like protein is one, but the LdMNPV-H2 and LdMNPV-T3 isolates have two of this gene.…”

Section: Discussionmentioning

confidence: 99%

Genome Sequencing and Organization of Three Geographically Different Isolates of Nucleopolyhedrovirus from the Gypsy Moth Reveal Significant Genomic Differences

Gencer,

Inan,

Bayramoglu

et al. 2023

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Background: The gypsy moth (Lymantria dispar L., Lepidoptera: Erebidae) is a worldwide pest of trees and forests. Lymantria dispar nucleopolyhedrovirus (LdMNPV) belongs to the Baculoviridae family and is an insect virus specific to gypsy moth larvae. In this study, we describe the complete genome sequences of three geographically diverse isolates, H2 (China), J2 (Japan), and T3 (Turkey), of Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV). Methods: The genomes of isolates H2, J2, and T3 were subjected to shotgun pyrosequencing using Roche 454 FLX and assembled using Roche GS De Novo Assembler. Comparative analysis of all isolates was performed using bioinformatics methods. Results: The genomes of LdMNPV-H2, J2, and T3 were 164,746, 162,249, and 162,614 bp in size, had GC content of 57.25%, 57.30%, and 57.46%, and contained 162, 165, and 164 putative open reading frames (ORFs ≥ 150 nt), respectively. Comparison between the reference genome LdMNPV-5/6 (AF081810) and the genomes of LdMNPV-H2, J2, and T3 revealed differences in gene content. Compared with LdMNPV-5/6, ORF5, 6, 8, 10, 31, and 67 were absent in LdMNPVH2, ORF5, 13, and 66 were absent in LdMNPV-J2, and ORF10, 13, 31, and 67 were absent in LdMNPV-T3. In addition, the gene encoding the mucin-like protein (ORF4) was split into two parts in isolates H2 and T3 and designated ORF4a and ORF4b. Phylogenetic analysis grouped isolates H2 and J2 in a different cluster than isolate T3, which is more closely related to the Turkish and Polish isolates. In addition, H2 was found to be closely related to a South Korean LdMNPV isolate. Conclusion: This study provided a more detailed overview of the relationships between different geographic LdMNPV isolates. The results showed remarkable differences between groups at the genome level.

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Section: Discussionmentioning

confidence: 99%

Genome Sequencing and Organization of Three Geographically Different Isolates of Nucleopolyhedrovirus from the Gypsy Moth Reveal Significant Genomic Differences

Gencer,

Inan,

Bayramoglu

et al. 2023

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Comparative genomics of the Neodiprion sertifer nucleopolyhedrovirus from Turkey with the fewest ORFs among baculoviruses

Doğan,

Budak,

Salman

et al. 2024

Virus Genes

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The complete genome of the European pine saw y Neodiprion sertifer nucleopolyhedrovirus was sequenced and characterized from next-generation sequencing data of the N. sertifer larva from Turkiye. This genome was comparatively analysed by previously reported genomes baculoviruses. The baculovirus phylogeny was reconstructed and the species boundary of the NeseNPV-TR was delineated using K2P distance. The length of the genome was 82,052 bp, with a G + C content of 33.28%. It contained 82 putative ORFs, including 33 baculovirus core genes, three lepidopteran baculovirus core genes, three non-conserved genes. It had ve hrs with 20.6% overall mean distance on average. The pairwise K2P distances of lef-9 and polh genes were lower than the speci ed threshold value, while those of lef-8, combinations of three genes and 33 genes were slightly higher between NeseNPV-TR and NeseNPV. The most variable genes were lef-2, helicase, p40, desmoplakin, p6.9, vp91 and vp39, while the most conserved were lef-8, lef-9, odv-e18, pif2 and lef-5 among baculoviruses. The genome of NeseNPV-TR is smaller and contains the fewest ORFs among baculoviruses. Some of unassigned ORFs had conserved domains and hence, we suggest further investigation to determine their structural and functional roles. Phylogenetic analyses con rmed its position within Gammabaculovirus. The NeseNPV-TR can be considered as the same species with NeseNPV. The different divergence rates in the baculovirus core genes may be related with different selection pressures acting on the genes. The lower genetic diversity of Group I alphabaculoviruses is most probably due to recent emergence.

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Exploitation of receptor tyrosine kinases by viral-encoded growth factors

Lateef

Wise

2018

Growth Factors

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Complete Genome Sequence of Lymantria dispar multiple nucleopolyhedrovirus Isolated in Southwestern Poland

Cited by 4 publications

References 3 publications

Genome Sequencing and Organization of Three Geographically Different Isolates of Nucleopolyhedrovirus from the Gypsy Moth Reveal Significant Genomic Differences

Genome Sequencing and Organization of Three Geographically Different Isolates of Nucleopolyhedrovirus from the Gypsy Moth Reveal Significant Genomic Differences

Comparative genomics of the Neodiprion sertifer nucleopolyhedrovirus from Turkey with the fewest ORFs among baculoviruses

Exploitation of receptor tyrosine kinases by viral-encoded growth factors

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