Complete Genome Sequence of
            <i>Edwardsiella piscicida</i>
            Isolate S11-285 Recovered from Channel Catfish (
            <i>Ictalurus punctatus</i>
            ) in Mississippi, USA

Reichley, Stephen R.; Waldbieser, Geoffrey C.; Tekedar, Hasan C.; Lawrence, Mark L.; Griffin, Matt J.

doi:10.1128/genomea.01259-16

Cited by 19 publications

(17 citation statements)

References 26 publications

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“…The final trees were constructed from 1,000 bootstrap replicates. Additionally, intragenomic heterogeneity of the 16S rRNA was evaluated by BLASTN searches of 16S rRNA sequences against the complete genomes of isolates E. anguillarum LADL05-105, E. hoshinae ATCC 35051, E. piscicida S11-285, and E. tarda FL95-01, which were closed ancillary to the current project (43)(44)(45)86), as well as E. ictaluri 93-146 (87). Genetic fingerprinting.…”

Section: Methodsmentioning

confidence: 99%

Comparative Phenotypic and Genotypic Analysis of Edwardsiella Isolates from Different Hosts and Geographic Origins, with Emphasis on Isolates Formerly Classified as E. tarda, and Evaluation of Diagnostic Methods

et al. 2017

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spp. are responsible for significant losses in important wild and cultured fish species worldwide. Recent phylogenomic investigations have determined that bacteria historically classified as actually represent three genetically distinct yet phenotypically ambiguous taxa with various degrees of pathogenicity in different hosts. Previous recognition of these taxa was hampered by the lack of a distinguishing phenotypic character. Commercial test panel configurations are relatively constant over time, and as new species are defined, appropriate discriminatory tests may not be present in current test panel arrangements. While phenobiochemical tests fail to discriminate between these taxa, data presented here revealed discriminatory peaks for each species using matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) methodology, suggesting that MALDI-TOF can offer rapid, reliable identification in line with current systematic classifications. Furthermore, a multiplex PCR assay was validated for rapid molecular differentiation of the spp. affecting fish. Moreover, the limitations of relying on partial 16S rRNA for discrimination of spp. and advantages of employing alternative single-copy genes and for molecular identification and classification of were demonstrated. Last, sequencing confirmed that isolates previously defined as typical motile fish-pathogenic are synonymous with, while atypical nonmotile fish-pathogenic isolates are equivalent to Fish-nonpathogenic isolates are consistent with as it is currently defined. These analyses help deconvolute the scientific literature regarding these organisms and provide baseline information to better facilitate proper taxonomic assignment and minimize erroneous identifications of isolates in clinical and research settings.

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Section: Methodsmentioning

confidence: 99%

Comparative Phenotypic and Genotypic Analysis of Edwardsiella Isolates from Different Hosts and Geographic Origins, with Emphasis on Isolates Formerly Classified as E. tarda, and Evaluation of Diagnostic Methods

et al. 2017

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“…Isolates have been previously characterized phenotypically and genetically by Griffin et al (, ) and Reichley et al (, , ).…”

Section: Methodsmentioning

confidence: 99%

Comparative Susceptibility of Channel Catfish, Ictalurus punctatus; Blue Catfish, Ictalurus furcatus; and Channel (♀) × Blue (♂) Hybrid Catfish to Edwardsiella piscicida, Edwardsiella tarda, and Edwardsiella anguillarum

Reichley

Ware

Khoo

et al. 2017

J World Aquaculture Soc

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Members of the genus Edwardsiella are important pathogens of cultured and wild fish globally. Recent investigations into the phenotypic and genotypic variation of Edwardsiella tarda have led to the segregation of E. tarda into three distinct taxa: E. tarda, Edwardsiella piscicida, and Edwardsiella anguillarum. In catfish aquaculture in the southeastern USA, E. piscicida has been more commonly associated with disease than E. tarda or E. anguillarum, and recent research has demonstrated E. piscicida to be more pathogenic in channel catfish than E. tarda or E. anguillarum. Anecdotal reports from industry suggest an increased prevalence of E. piscicida associated with the culture of channel (♀) × blue (♂) hybrid catfish. This work investigated the comparative susceptibility of channel catfish, blue catfish, and their hybrid cross to molecularly confirmed isolates of E. tarda, E. piscicida, and E. anguillarum. There was significantly higher mortality in hybrid catfish compared to channel catfish following intracoelomic injection of E. piscicida. To our knowledge, E. piscicida is the first bacterial pathogen to demonstrate increased pathogenicity in hybrid catfish compared to channel catfish. KEYWORDS blue catfish, channel catfish, Edwardsiella, hybrid catfish

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“…Genetic fingerprints of case isolates (R18‐14‐1; R18‐14‐2; R18‐14‐13; R18‐14‐16; R18‐35‐2) with other Edwardsiella anguillarum isolates recovered from the same farm (Eco2413; Eco2417) and an outbreak in cultured tilapia in Colombia (R17‐63). Reference: E. anguillarum LADL 05–105 (Reichley, Waldbieser, Lawrence, et al, ), E. piscicida S11‐285 (Reichley, Waldbieser, Tekedar, Lawrence, & Griffin, ) and E. tarda FL95‐01 (Reichley, Waldbieser, Tekedar, Lawrence, & Griffin, ) isolates , as well as an E. coli reference isolate, were included in the analysis. Amplification reactions were performed using: (a) Edwardsiella anguillarum ‐specific primers, (b) ERIC I & II primers and (c) BOX primers.…”

Section: Antimicrobial Minimum Inhibitory Concentrations (Mics) Againmentioning

confidence: 99%

“…Reference: E. anguillarum LADL 05-105 , E. piscicida S11-285 (Reichley, Waldbieser, Tekedar, Lawrence, & Griffin, 2016) and E. tarda FL95-01 (Reichley, Waldbieser, Tekedar, Lawrence, & Griffin, 2015) isolates, as well as an E. coli reference isolate, were included in the analysis. Amplification reactions were performed using: (a) Edwardsiella anguillarum-specific primers, (b) ERIC I & II primers and (c) BOX primers.…”

mentioning

confidence: 99%

Pathologic changes in cultured Nile tilapia (Oreochromis niloticus) associated with an outbreak of Edwardsiella anguillarum

Armwood

Camus

López-Porras

et al. 2019

Journal of Fish Diseases

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Complete Genome Sequence of Edwardsiella piscicida Isolate S11-285 Recovered from Channel Catfish ( Ictalurus punctatus ) in Mississippi, USA

Cited by 19 publications

References 26 publications

Comparative Phenotypic and Genotypic Analysis of Edwardsiella Isolates from Different Hosts and Geographic Origins, with Emphasis on Isolates Formerly Classified as E. tarda, and Evaluation of Diagnostic Methods

Comparative Phenotypic and Genotypic Analysis of Edwardsiella Isolates from Different Hosts and Geographic Origins, with Emphasis on Isolates Formerly Classified as E. tarda, and Evaluation of Diagnostic Methods

Comparative Susceptibility of Channel Catfish, Ictalurus punctatus; Blue Catfish, Ictalurus furcatus; and Channel (♀) × Blue (♂) Hybrid Catfish to Edwardsiella piscicida, Edwardsiella tarda, and Edwardsiella anguillarum

Pathologic changes in cultured Nile tilapia (Oreochromis niloticus) associated with an outbreak of Edwardsiella anguillarum

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