Complete Genome Sequence of
            <i>Aeromonas</i>
            Phage ZPAH7 with Halo Zones, Isolated in China

Islam, Mohammad Sharifull; Raz, Assaf; Liu, Yang; Elbassiony, Khalid Ragaei Abdraboh; Dong, Xingxing; Zhou, Peng; Zhou, Yang; Li, Jinquan

doi:10.1128/mra.01678-18

Cited by 17 publications

(12 citation statements)

References 10 publications

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“…For intance, Ackermann [23] indicated that 33 of 43 Aeromonas phages investigated belonged to the Myoviridae family. Also, several Aeromonas phages belonging to the family Podoviridae have been isolated and characterized [34][35][36]. Phage Y71 and Y81 isolated in this study, possessing an icosahedral head with a short noncontractile tail typical of the Podoviridae, were tentatively classified as members of this family.…”

Section: Discussionmentioning

confidence: 91%

Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila

Jin

Gao

Dong

et al. 2020

Preprint

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Background: Aeromonas hydrophila is an important water-borne pathogen that leads to a great economic loss in aquaculture. Along with the abuse of antibiotics, drug-resistant strains rise rapidly. In addition, the biofilms formed by this bacterium limited the antibacterial effect of antibiotics. Bacteriophages have been attracting increasing attention as a potential alternative to antibiotics against bacterial infections. Results: Five phages against pathogenic A. hydrophila, named N21, W3, G65, Y71 and Y81, were isolated. Morphological analysis by transmission electron microscopy revealed that phages N21, W3 and G65 belong to the family Myoviridae, while Y71 and Y81 belong to the Podoviridae. These phages were found to have broad host spectra, short latent periods and normal burst sizes. They were sensitive to high temperature but had a wide adaptability to the pH. In addition, the phages G65 and Y81 showed considerable bacterial killing effect and potential in preventing formation of A. hydrophila biofilm; and the phages G65, W3 and N21 were able to scavenge mature biofilm effectively. Phage treatments applied to the pathogenic A. hydrophila in mice model resulted in a significantly decreased bacterial loads in tissues. Conclusions: Five A. hydrophila phages were isolated with broad host ranges, low latent periods, and wide pH and thermal tolerance. And the phages exhibited varying abilities in controlling A. hydrophila infection. This work presents promising data supporting the future use of phage therapy.

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Section: Discussionmentioning

confidence: 91%

Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila

Jin

Gao

Dong

et al. 2020

Preprint

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“…As examined by TEM, three phages, LPSTLL, LPST94 and LPST153, belonged to the order of Caudovirales and family of Siphoviridae , Ackermannviridae and Podoviridae, respectively. Phages belong to these families all have the potential, as suggested by other literatures, to apply as biocontrol candidates against Salmonella and their biofilms [81,82,83,84,85,86].…”

Section: Discussionmentioning

confidence: 99%

Application of a Phage Cocktail for Control of Salmonella in Foods and Reducing Biofilms

Islam

Zhou

Liang

et al. 2019

Viruses

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Salmonella contamination in foods and their formation of biofilms in food processing facility are the primary bacterial cause of a significant number of foodborne outbreaks and infections. Broad lytic phages are promising alternatives to conventional technologies for pathogen biocontrol in food matrices and reducing biofilms. In this study, 42 Salmonella phages were isolated from environmentally-sourced water samples. We characterized the host range and lytic capacity of phages LPSTLL, LPST94 and LPST153 against Salmonella spp., and all showed a wide host range and broad lytic activity. Electron microscopy analysis indicated that LPSTLL, LPST94, and LPST153 belonged to the family of Siphoviridae, Ackermannviridae and Podoviridae, respectively. We established a phage cocktail containing three phages (LPSTLL, LPST94 and LPST153) that had broad spectrum to lyse diverse Salmonella serovars. A significant decrease was observed in Salmonella with a viable count of 3 log10 CFU in milk and chicken breast at either 25 °C or 4 °C. It was found that treatment with phage cocktail was able to significantly reduced biofilm on a 96-well microplate (44–63%) and on a stainless steel surface (5.23 to 6.42 log10). These findings demonstrated that the phage cocktail described in this study can be potentially used as a biological control agent against Salmonella in food products and also has the effect to reduce Salmonella formed biofilms.

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“…Since the presence of halos around the clear plaque zone is believed to be a decent indication that the diffusion of the phage enzymatic molecules (such as EPS depolymerases) can be effective in dispersing biofilms [ 50 ]. These phages have biotechnological applications in the treatment because it is possible that biofilms were controlled by phage infection and phage has the ability to produce (or to be able to induce) enzymes that can degrade extracellular matrix [ 52 , 53 ]. Hughes et al have reported that the presence activity of polysaccharide depolymerases from phage SF153b might be the reason to be effective against Enterobacter agglomerans biofilms [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Salmonella Phage LPST153 That Effectively Targets Most Prevalent Salmonella Serovars

Islam

Mizan

et al. 2020

Microorganisms

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Foodborne diseases represent a major risk to public health worldwide. In this study, LPST153, a novel Salmonella lytic phage with halo (indicative of potential depolymerase activity) was isolated by employing Salmonella enterica serovar Typhimurium ATCC 13311 as the host and had excellent lytic potential against Salmonella. LPST153 is effectively able to lyse most prevalent tested serotypes of Salmonella, including S. Typhimurium, S. Enteritidis, S. Pullorum and S. Gallinarum. Morphological analysis revealed that phage LPST153 belongs to Podoviridae family and Caudovirales order and could completely prevent host bacterial growth within 9 h at multiplicity of infection (MOI) of 0.1, 1, 10 and 100. LPST153 had a latent period of 10 min and a burst size of 113 ± 8 PFU/cell. Characterization of the phage LPST153 revealed that it would be active and stable in some harsh environments or in different conditions of food processing and storage. After genome sequencing and phylogenetic analysis, it is confirmed that LPST153 is a new member of the Teseptimavirus genus of Autographivirinae subfamily. Further application experiments showed that this phage has potential in controlling Salmonella in milk and sausage. LPST153 was also able to inhibit the formation of biofilms and it had the ability to reduce and kill bacteria from inside, including existing biofilms. Therefore, the phage LPST153 could be used as a potential antibacterial agent for Salmonella control in the food industry.

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Complete Genome Sequence of Aeromonas Phage ZPAH7 with Halo Zones, Isolated in China

Abstract: Phage ZPAH7, isolated from a sediment sample from a fish farm, is a novel lytic phage belonging to the Podoviridae family. It produces large plaques (3.5 ± 0.2 mm) with halo zones (10.5 ± 0.5 mm), suggesting it has the ability to depolymerize exopolysaccharides and biofilms.

Cited by 17 publications

References 10 publications

Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila

Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila

Application of a Phage Cocktail for Control of Salmonella in Foods and Reducing Biofilms

Characterization of Salmonella Phage LPST153 That Effectively Targets Most Prevalent Salmonella Serovars

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