Complete genome sequence of Granulosicoccus antarcticus type strain IMCC3135T, a marine gammaproteobacterium with a putative dimethylsulfoniopropionate demethylase gene

Kang, Ilnam; Lim, Yeonjung; Cho, Jang-Cheon

doi:10.1016/j.margen.2017.11.005

Cited by 24 publications

(22 citation statements)

References 35 publications

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“…supports a diverse range of epiphytic bacteria from phyla Proteobacteria, Bacteroidetes, Planctomycetes, Actinobacteria, Firmicutes and Verrucomicrobia, all of which are known to associate with macroalgal surfaces [31,50,51]. Previous studies also report that some bacterial genera identified here, including Winogradskyella [52], Granulosicoccus [53] and Aquimarina [54], possess features such as alginate degradation which would contribute to their growth and survival on the surface of alginate-rich brown algae such as Laminaria digitata. Furthermore, although equal volumes of nucleic acids were pooled from three separate individuals (n = 3 individuals per algal region per sampling time) and triplicate PCRs, these were not precisely equimolar.…”

Section: Plos Onesupporting

confidence: 64%

Diversity of bacteria populations associated with different thallus regions of the brown alga Laminaria digitata

Ihua

FitzGerald

Guihéneuf³

et al. 2020

PLoS ONE

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Stipitate kelp species such as Laminaria digitata dominate most cold-water subtidal rocky shores and form underwater forests which are among the most productive coastal systems worldwide. Laminaria also sustains rich bacterial communities which offer a variety of biotechnological applications. However, to date, in-depth studies on the diversity and uniqueness of bacterial communities associated with this macroalgal species, their ecological role and their interactions with the alga are under-represented. To address this, the epibacterial populations associated with different thallus regions (holdfast, stipe, meristem, blade) of this brown seaweed were investigated using high-throughput Illumina sequencing of the 16S rRNA genes. The results show that epibacterial communities of the brown seaweed are significantly different and specific to the thallus region, with the shared bacterial population comprising of only 1.1% of the total amplicon sequence variants. The diverse holdfast and blade tissues formed distinct clusters while the meristem and stipe regions are more closely related. The data obtained further supports the hypothesis that macroalgal bacterial communities are shaped by morphological niches and display specificity.

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Section: Plos Onesupporting

confidence: 64%

Diversity of bacteria populations associated with different thallus regions of the brown alga Laminaria digitata

Ihua

FitzGerald

Guihéneuf³

et al. 2020

PLoS ONE

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“…Whether DMSP is made by Fucus or its epiphytes, it clearly has an effect on the holobiont's microbial community. For example, Granulosicoccus and Octadecabacter both possess genes for DMSP degradation (Kang et al, 2018;Zeng and Qiao, 2019), and they account for 10% of all ASVs in our study. These genes may contribute to the presence of these genera on many invertebrates and macroalgae (e.g., Miranda et al, 2013;Roth-Schulze et al, 2018;Lemay et al, 2018;van de Water et al, 2018;Weigel and Pfister, 2019).…”

Section: Discussionmentioning

confidence: 75%

Bacterial Communities Show Algal Host (Fucus spp.)/Zone Differentiation Across the Stress Gradient of the Intertidal Zone

et al. 2020

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“…The genus Granulosicoccus was prevalent in the oral cavity samples of the wild horseshoe crabs and is present in the microbiomes of several other marine organisms, such as seagrass ( Kurilenko et al, 2010 ), kelp ( Bengtsson et al, 2012 ), and corals ( van Bleijswijk et al, 2015 ). A genomic analysis of the type strain Granulosicoccus antarcticus IMCC3135T (=KCCM 42676 T = NBRC 102684 T ) revealed the presence of several genes associated with sulfur cycling; for example, the genome contained a gene encoding for the enzyme dimethylsulfoniopropionate (DMSP) demethylase and several genes associated with sulfur oxidation ( Kang et al, 2018 ). These bacteria are likely responsible for a complex sulfur biogeochemical cycle in and on wild horseshoe crabs.…”

Section: Discussionmentioning

confidence: 99%

Microbiome Shifts Associated With the Introduction of Wild Atlantic Horseshoe Crabs (Limulus polyphemus) Into a Touch-Tank Exhibit

et al. 2020

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The Atlantic horseshoe crab ( Limulus polyphemus ) is a common marine aquarium species and model organism for research. There is potential monetary and conservation value in developing a stable captive population of horseshoe crabs, however, one major impediment to achieving captivity is a lack of knowledge regarding captive diseases. We utilized 16S rRNA gene amplicon sequencing to track changes in the microbiomes of four body locations in three wild-caught (tracked over 14 months in captivity) and three tank-acclimated (>2 years in captivity) adult L. polyphemus in a touch tank at Shark Reef Aquarium at Mandalay Bay in Las Vegas, NV. The wild population hosted diverse and distinct microbiomes on the carapace (260 ± 96 amplicon sequence variants or ASVs), cloaca (345 ± 77 ASVs), gills (309 ± 36 ASVs), and oral cavity (359 ± 37 ASVs), which were dominated by classes Gammaproteobacteria , Bacteroidia , and Alphaproteobacteria . A rapid decline in richness across all body locations was observed within 1 month of captivity, with tank-acclimated (>2 years) animals having <5% of the initial microbiome richness and a nearly completely restructured microbial community. Tank-acclimated horseshoe crabs possessed distinct microbiomes that were highly uneven and low in species richness on the carapace (31 ± 7 ASVs), cloaca (53 ± 19 ASVs), gills (17 ± 2 ASVs), and oral cavity (31 ± 13 ASVs). The carapace, oral cavity, and gills of the tank-acclimated animals hosted abundant populations of Aeromonas (>60%) and Pseudomonas (>20%), both of which are known opportunistic pathogens of aquatic animals and can express chitinases, providing a plausible mechanism for the development of the carapace lesion pathology observed in this and other studies. The cloaca of the tank-acclimated animals was slightly more diverse than the other body locations with Aeromonas , Enterococcus , Shewanella , and Vagococcus dominating the community. These results provide an important baseline on the microbiomes of both wild and tank-acclimated horseshoe crabs and underscore the need to continue to investigate how native microbial populations may protect animals from pathogens.

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Complete genome sequence of Granulosicoccus antarcticus type strain IMCC3135T, a marine gammaproteobacterium with a putative dimethylsulfoniopropionate demethylase gene

Cited by 24 publications

References 35 publications

Diversity of bacteria populations associated with different thallus regions of the brown alga Laminaria digitata

Diversity of bacteria populations associated with different thallus regions of the brown alga Laminaria digitata

Bacterial Communities Show Algal Host (Fucus spp.)/Zone Differentiation Across the Stress Gradient of the Intertidal Zone

Microbiome Shifts Associated With the Introduction of Wild Atlantic Horseshoe Crabs (Limulus polyphemus) Into a Touch-Tank Exhibit

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